Cloning and heterologous expression of chlorophyll a synthase in Rhodobacter sphaeroides

Date

2017-03-01

Author

Ipekoglu, Emre M.
Gocmen, Koray
Oz, Mehmet T.
Gurgan, Muazzez
Yucel, Meral

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Rhodobacter sphaeroides is a purple non-sulfur bacterium which photoheterotrophically produces hydrogen from organic acids under anaerobic conditions. A gene coding for putative chlorophyll a synthase (chlG) from cyanobacterium Prochlorococcus marinus was amplified by nested polymerase chain reaction and cloned into an inducible-expression plasmid which was subsequently transferred to R. sphaeroides for heterologous expression. Induced expression of chlG in R. sphaeroides led to changes in light absorption spectrum within 400-700nm. The hydrogen production capacity of the mutant strain was evaluated on hydrogen production medium with 15mM malate and 2mM glutamate. Hydrogen yield and productivity were increased by 13.6 and 22.6%, respectively, compared to the wild type strain. The results demonstrated the feasibility of genetic engineering to combine chlorophyll and bacteriochlorophyll biosynthetic pathways which utilize common intermediates. Heterologous expression of key enzymes from biosynthetic pathways of various pigments is proposed here as a general strategy to improve absorption spectra and yield of photosynthesis and hydrogen gas production in bacteria.

Subject Keywords

Chlorophyll A Synthase, Hydrogen Production, Photosynthesis, Prochlorococcus Marinus, Rhodobacter Sphaeroides

URI

https://hdl.handle.net/11511/68199

Journal

JOURNAL OF BASIC MICROBIOLOGY

DOI

https://doi.org/10.1002/jobm.201600580

Collections

Department of Biology, Article

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Citation Formats

E. M. Ipekoglu, K. Gocmen, M. T. Oz, M. Gurgan, and M. Yucel, “Cloning and heterologous expression of chlorophyll a synthase in Rhodobacter sphaeroides,” JOURNAL OF BASIC MICROBIOLOGY, pp. 238–244, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/68199.