Photoproduction of hydrogen from sugar refinery wastewater by Rhodobacter sphaeroides OU 001

Yetis, M
Gündüz, Ufuk
Eroglu, I
Yucel, M
Turker, L
Pretreated sugar refinery wastewater (SRWW) was used for the production of hydrogen by Rhodobacter sphaeroides O.U.001 in a 0.4 1 column photobioreactor. Hydrogen was produced at a rate of 0.001 1 hydrogen/h/l culture in 20% dilution of SRWW. To adjust the carbon concentration to 70 mM and nitrogen concentration to 2 mM, sucrose or l-malic acid was added as carbon source and sodium glutamate was added as nitrogen source to the 20% dilution of SRWW. By these adjustments, hydrogen production rate was increased to 0.005 1 hydrogen/h/l culture. Continuous hydrogen production was achieved in the same medium for 100 days at three different dilution rates. The maximum hydrogen produced was 2.67 1 tin 100 days) at a dilution rate of 0.0013 h(-1) (C) 2000 international Association for Hydrogen Energy. Published by Elsevier Science Ltd. All rights reserved.


Photoproduction of hydrogen by Rhodobacter capsulatus from thermophilic fermentation effluent
Uyar, Basar; Schumacher, Matthias; Gebicki, Jakub; Modigell, Michael (2009-08-01)
Rhodobacter capsulatus was used for the phototrophic hydrogen production on effluent solution derived from the thermophilic fermentation of Miscanthus hydrolysate by Thermotoga neapolitana. Pretreatments such as centrifugation, dilution, buffer addition, pH adjustment and sterilization were suggested for the effluent before being fed to the photofermentation. Batch-wise experiments showed that R. capsulatus grows and produces hydrogen on the pretreated effluent solution. Moreover, it was found that the hydr...
Continuous hydrogen production by Rhodobacter sphaeroides O.U.001
Eroğlu, İnci; Aslan, K; Gunduz, M; Yucel, M; Turker, L (1997-06-26)
This paper describes hydrogen gas production by Rhodobacter sphaeroides O.U.001 using a column photobioreactor in batch and continuous operation. The effect of substrates on the hydrogen production rate was investigated in batch-type photobioreactor experiments. Substrate concentrations (L-malic acid and sodium glutamate) were measured by using high-pressure liquid chromatography. The gas produced was analyzed by gas chromatography.
Photobiological hydrogen production by Rhodobacter sphaeroides O.U.001 by utilization of waste water from milk industry
Turkarslan, S; Yigit, DO; Aslan, K; Eroğlu, İnci; Gündüz, Ufuk (1997-06-26)
Hydrogen production with photosynthetic microorganisms contributes to the protection of the environment, not only in producing a clean fuel but also in waste treatment processes. In this study, Rhodobacter sphaeroides, a photosynthetic bacteria, is used in photobiological hydrogen production by using waste water from the milk industry.
Substrate consumption rates for hydrogen production by Rhodobacter sphaeroides in a column photobioreactor
Eroglu, I; Aslan, K; Gündüz, Ufuk; Yucel, M; Turker, L (1999-04-30)
The effect of L-malic acid and sodium glutamate, which serve as the carbon and nitrogen source, respectively, on hydrogen production by Rhodobacter sphaeroides O.U.001 has been investigated in a batch water jacketed glass column photobioreactor (PBR), which has an inner volume of 400 ml. The PER was operated at different carbon to nitrogen ratios at 32 degrees C with a tungsten lamp at a light intensity of 200 W m(-2). Carbon to nitrogen ratio was found to be an important parameter for bio-hydrogen producti...
Photoelectrochemical H-2 production by using bacteriorhodopsin immobilized in PAG
Yucel, M; El-Bashiti, T; Eroğlu, İnci; Sediroglu, V; Turker, L; Gündüz, Ufuk (2000-06-15)
Bacteriorhodopsin (BR), a protein found in the purple membrane of Halobacterium halobium is used as a biocatalyst in photoelectrochemical hydrogen production in a NaCl solution. In present study the biocatalytic effect of BR of a new strain MILTU and strain S-9 were investigated in immobilized membranes The photoelectrochemical hydrogen production experiments were performed in a batch type glass reactor with free H. halobium packed cells or H halobium cells immobilized in polyacrylamide gel (PAG). It was fo...
Citation Formats
M. Yetis, U. Gündüz, I. Eroglu, M. Yucel, and L. Turker, “Photoproduction of hydrogen from sugar refinery wastewater by Rhodobacter sphaeroides OU 001,” INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, pp. 1035–1041, 2000, Accessed: 00, 2020. [Online]. Available: