Substrate consumption rates for hydrogen production by Rhodobacter sphaeroides in a column photobioreactor

Eroglu, I
Aslan, K
Gündüz, Ufuk
Yucel, M
Turker, L
The effect of L-malic acid and sodium glutamate, which serve as the carbon and nitrogen source, respectively, on hydrogen production by Rhodobacter sphaeroides O.U.001 has been investigated in a batch water jacketed glass column photobioreactor (PBR), which has an inner volume of 400 ml. The PER was operated at different carbon to nitrogen ratios at 32 degrees C with a tungsten lamp at a light intensity of 200 W m(-2). Carbon to nitrogen ratio was found to be an important parameter for bio-hydrogen production. Moreover, hydrogen gas production was dependent on certain threshold concentrations of sodium glutamate. L-malic acid consumption was found to be first order with respect to L-malic acid concentration, whereas sodium glutamate consumption was found to be second order with respect to glutamate concentration. It was concluded that there is a close relationship between the hydrogen production rate and substrate consumption rates. A kinetic model is developed, which relates hydrogen gas production per amount of biomass, L-malic acid, and sodium glutamate concentrations.


Photoproduction of hydrogen from sugar refinery wastewater by Rhodobacter sphaeroides OU 001
Yetis, M; Gündüz, Ufuk; Eroglu, I; Yucel, M; Turker, L (2000-11-01)
Pretreated sugar refinery wastewater (SRWW) was used for the production of hydrogen by Rhodobacter sphaeroides O.U.001 in a 0.4 1 column photobioreactor. Hydrogen was produced at a rate of 0.001 1 hydrogen/h/l culture in 20% dilution of SRWW. To adjust the carbon concentration to 70 mM and nitrogen concentration to 2 mM, sucrose or l-malic acid was added as carbon source and sodium glutamate was added as nitrogen source to the 20% dilution of SRWW. By these adjustments, hydrogen production rate was increase...
Continuous hydrogen production by Rhodobacter sphaeroides O.U.001
Eroğlu, İnci; Aslan, K; Gunduz, M; Yucel, M; Turker, L (1997-06-26)
This paper describes hydrogen gas production by Rhodobacter sphaeroides O.U.001 using a column photobioreactor in batch and continuous operation. The effect of substrates on the hydrogen production rate was investigated in batch-type photobioreactor experiments. Substrate concentrations (L-malic acid and sodium glutamate) were measured by using high-pressure liquid chromatography. The gas produced was analyzed by gas chromatography.
Kinetic analysis of photosynthetic growth, hydrogen production and dual substrate utilization by Rhodobacter capsulatus
Sevinc, Pelin; Gündüz, Ufuk; EROĞLU, İNCİ; Yucel, Meral (2012-11-01)
Rhodobacter capsulatus is purple non-sulfur (PNS) bacterium which can produce hydrogen and CO2 by utilizing volatile organic acids in presence of light under anaerobic conditions. Photofermentation by PNS bacteria is strongly affected by temperature and light intensity. In the present study we present the kinetic analysis of growth, hydrogen production, and dual consumption of acetic acid and lactic acid at different temperatures (20, 30 and 38 degrees C) and light intensities (1500, 2000, 3000, 4000 and 50...
Direct synthesis of hydrogen storage alloys from their oxides
Tan, Serdar; Öztürk, Tayfur; Aydınol, Mehmet Kadri; Department of Metallurgical and Materials Engineering (2011)
The aim of this study is the synthesis of hydrogen storage compounds by electrodeoxidation technique which offers an inexpensive and rapid route to synthesize compounds from oxide mixtures. Within the scope of this study, two hydrogen storage compounds, FeTi and Mg2Ni, are aimed to be produced by this technique. In the first part, effect of sintering conditions on synthesis of FeTi was studied. For this purpose, oxide pellets made out of Fe2O3-TiO2 powders were sintered at temperatures between 900 °C – 1300...
Kinetics of biological hydrogen production by the photosynthetic bacterium Rhodobacter sphaeroides O.U. 001
Koku, Harun; Gündüz, Ufuk; Türker, Lemi (2003-04-01)
The kinetics and the effects of various parameters on hydrogen production by Rhodobacter sphaeroides O.U. 001 were investigated in a batch column photobioreactor. In particular, the effect of the inoculum age and the implementation of a light-dark cycle illumination scheme for emulating natural sunlight have been investigated in detail. The possibility of using yeast extract to replace the rather expensive vitamin mixture in the medium was also studied. The results show that hydrogen production is decrease...
Citation Formats
I. Eroglu, K. Aslan, U. Gündüz, M. Yucel, and L. Turker, “Substrate consumption rates for hydrogen production by Rhodobacter sphaeroides in a column photobioreactor,” JOURNAL OF BIOTECHNOLOGY, pp. 103–113, 1999, Accessed: 00, 2020. [Online]. Available: