Substrate consumption rates for hydrogen production by Rhodobacter sphaeroides in a column photobioreactor

Date

1999-04-30

Author

Eroglu, I
Aslan, K
Gündüz, Ufuk
Yucel, M
Turker, L

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The effect of L-malic acid and sodium glutamate, which serve as the carbon and nitrogen source, respectively, on hydrogen production by Rhodobacter sphaeroides O.U.001 has been investigated in a batch water jacketed glass column photobioreactor (PBR), which has an inner volume of 400 ml. The PER was operated at different carbon to nitrogen ratios at 32 degrees C with a tungsten lamp at a light intensity of 200 W m(-2). Carbon to nitrogen ratio was found to be an important parameter for bio-hydrogen production. Moreover, hydrogen gas production was dependent on certain threshold concentrations of sodium glutamate. L-malic acid consumption was found to be first order with respect to L-malic acid concentration, whereas sodium glutamate consumption was found to be second order with respect to glutamate concentration. It was concluded that there is a close relationship between the hydrogen production rate and substrate consumption rates. A kinetic model is developed, which relates hydrogen gas production per amount of biomass, L-malic acid, and sodium glutamate concentrations.

Subject Keywords

Hydrogen production, Photobioreactor, Rhodobacter sphaeroides, Instantaneous fractional yield, Hydrogen production factor, Dual substrate

URI

https://hdl.handle.net/11511/31634

Journal

JOURNAL OF BIOTECHNOLOGY

DOI

https://doi.org/10.1016/s0168-1656(99)00064-4

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

I. Eroglu, K. Aslan, U. Gündüz, M. Yucel, and L. Turker, “Substrate consumption rates for hydrogen production by Rhodobacter sphaeroides in a column photobioreactor,” JOURNAL OF BIOTECHNOLOGY, pp. 103–113, 1999, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31634.