Identification of by-products in hydrogen producing bacteria; Rhodobacter sphaeroides OU 001 grown in the waste water of a sugar refinery

Yigit, DO
Gündüz, Ufuk
Turker, L
Yucel, M
Eroglu, I
Rhodobacter sphaeroides O.U.001 is able to produce hydrogen anaerobically upon illumination. The cells were screened for the presence of valuable by-products such as poly-e-hydroxy (PHB) butyric acid aiming to improve the feasibility of the system. Also waste water from a sugar refinery was used for bacterial growth to further increase the feasibility. Under aerobic conditions the standard growth media containing L-malic acid and sodium glutamate in 7.5/10 and 15/2 molar ratios and a medium containing 30% waste water from sugar refinery were used, In this case the maximum concentration of PHB produced were approximately 0.2 g l(-1) in both of the standard media whereas it was 0.3 g l(-1) in medium containing 30% waste water. By using the medium containing 30% waste water, PHB and hydrogen productions were determined under anaerobic conditions. The maximum concentration of PHB produced was around 0.5 g l(-1) and the amount of gas collected was 35 mi in 108 h. From these results it can be concluded that PHB can be collected during hydrogen production. The use of waste water from sugar refinery increased the yield.


Hydrogen production by Rhodobacter sphaeroides OU001 in a flat plate solar bioreactor
Eroglu, Inci; Tabanoglu, Altan; Gündüz, Ufuk; Eroglu, Ela; Yucel, Meral (2008-01-01)
Rhodobacter sphaeroides O.U.001 can produce hydrogen under anaerobic conditions and illumination. The objective of this study was to investigate the performance of an 81 flat plate solar bioreactor operating in outdoor conditions. Different organic acids were used as carbon sources (malate, lactate and acetate) and olive mill waste water was used as a sole substrate source. The consumption and the production of the organic acids were determined by HPLC. The accumulation of by-products, such as poly-beta-hyd...
Effect of light intensity, wavelength and illumination protocol on hydrogen production in photobioreactors
Uyar, Basar; EROĞLU, İNCİ; Yucel, Meral; Gündüz, Ufuk; Turker, Lemi (2007-12-01)
Rhodobacter sphaeroides O.U. 001 is a purple non-sulfur bacterium which evolves hydrogen from the breakdown of organic acids under illumination and anaerobic conditions. In this study, the effect of light intensity, light wavelength and illumination protocol on the growth and hydrogen production of R. sphaeroides O.U. 001 was investigated in gas-tight glass photobioreactors with defined medium. The results showed Also it was found that the rate of hydrogen production increased with increasing light intensit...
Evaluation of hydrogen production by Rhodobacter sphaeroides OU001 and its hupSL deficient mutant using acetate and malate as carbon sources
KARS, GÖKHAN; Gündüz, Ufuk; Yucel, Meral; Rakhely, Gabor; Kovacs, Kornel L.; Eroglu, Inci (2009-03-01)
Rhodobacter sphaeroides O.U.001 is one of the candidates for photobiological hydrogen production among purple non-sulfur bacteria. Hydrogen is produced by Mo-nitrogenase from organic acids such as malate or lactate. A hupSL in frame deletion mutant strain was constructed without using any antibiotic resistance gene. The hydrogen production potential of the R. sphaeroides O.U.001 and its newly constructed hupSL deleted mutant strain in acetate media was evaluated and compared with malate containing media. Th...
Cloning and heterologous expression of chlorophyll a synthase in Rhodobacter sphaeroides
Ipekoglu, Emre M.; Gocmen, Koray; Oz, Mehmet T.; Gurgan, Muazzez; Yucel, Meral (2017-03-01)
Rhodobacter sphaeroides is a purple non-sulfur bacterium which photoheterotrophically produces hydrogen from organic acids under anaerobic conditions. A gene coding for putative chlorophyll a synthase (chlG) from cyanobacterium Prochlorococcus marinus was amplified by nested polymerase chain reaction and cloned into an inducible-expression plasmid which was subsequently transferred to R. sphaeroides for heterologous expression. Induced expression of chlG in R. sphaeroides led to changes in light absorption ...
Observation of Oscillatory Surface Reactions of Riboflavin, Trolox, and Singlet Oxygen Using Single Carbon Nanotube Fluorescence Spectroscopy
Sen, Fatih; Boghossian, Ardemis A.; Sen, Selda; Ulissi, Zachary W.; Zhang, Jingqing; Strano, Michael S. (2012-12-01)
Single-molecule fluorescent microscopy allows semiconducting single-walled carbon nanotubes (SWCNTs) to detect the adsorption and desorption of single adsorbate molecules as a stochastic modulation of emission intensity. In this study, we identify and assign the signature of the complex decomposition and reaction pathways of riboflavin in the presence of the free radical scavenger Trolox using DNA-wrapped SWCNT sensors dispersed onto an aminopropyltriethoxysilane (APTES) coated surface. SWCNT emission is qu...
Citation Formats
D. Yigit, U. Gündüz, L. Turker, M. Yucel, and I. Eroglu, “Identification of by-products in hydrogen producing bacteria; Rhodobacter sphaeroides OU 001 grown in the waste water of a sugar refinery,” JOURNAL OF BIOTECHNOLOGY, pp. 125–131, 1999, Accessed: 00, 2020. [Online]. Available: