Effect of light intensity, wavelength and illumination protocol on hydrogen production in photobioreactors

Date

2007-12-01

Author

Uyar, Basar
EROĞLU, İNCİ
Yucel, Meral
Gündüz, Ufuk
Turker, Lemi

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

291
views

0
downloads

Rhodobacter sphaeroides O.U. 001 is a purple non-sulfur bacterium which evolves hydrogen from the breakdown of organic acids under illumination and anaerobic conditions. In this study, the effect of light intensity, light wavelength and illumination protocol on the growth and hydrogen production of R. sphaeroides O.U. 001 was investigated in gas-tight glass photobioreactors with defined medium. The results showed Also it was found that the rate of hydrogen production increased with increasing light intensity and reached saturation at around 270W/m(2) that lack of infrared light (750-950 nm wavelength) decreased photoproduction of hydrogen by 39%. Another factor evaluated was the effect of different illumination protocols on the growth and hydrogen production. It was observed that illumination after inoculation stimulates hydrogen production, increases substrate conversion efficiency and hydrogen production rate; no hydrogen was produced during the dark periods. (c) 2007 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.

Subject Keywords

Biological hydrogen production, Rhodobacter sphaeroides, Photobioreactors, Photofermentation

URI

https://hdl.handle.net/11511/32258

Journal

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY

DOI

https://doi.org/10.1016/j.ijhydene.2007.07.002

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Kinetic analysis of photosynthetic growth, hydrogen production and dual substrate utilization by Rhodobacter capsulatus Sevinc, Pelin; Gündüz, Ufuk; EROĞLU, İNCİ; Yucel, Meral (2012-11-01) Rhodobacter capsulatus is purple non-sulfur (PNS) bacterium which can produce hydrogen and CO2 by utilizing volatile organic acids in presence of light under anaerobic conditions. Photofermentation by PNS bacteria is strongly affected by temperature and light intensity. In the present study we present the kinetic analysis of growth, hydrogen production, and dual consumption of acetic acid and lactic acid at different temperatures (20, 30 and 38 degrees C) and light intensities (1500, 2000, 3000, 4000 and 50...
Cloning and heterologous expression of chlorophyll a synthase in Rhodobacter sphaeroides Ipekoglu, Emre M.; Gocmen, Koray; Oz, Mehmet T.; Gurgan, Muazzez; Yucel, Meral (2017-03-01) Rhodobacter sphaeroides is a purple non-sulfur bacterium which photoheterotrophically produces hydrogen from organic acids under anaerobic conditions. A gene coding for putative chlorophyll a synthase (chlG) from cyanobacterium Prochlorococcus marinus was amplified by nested polymerase chain reaction and cloned into an inducible-expression plasmid which was subsequently transferred to R. sphaeroides for heterologous expression. Induced expression of chlG in R. sphaeroides led to changes in light absorption ...
Hydrogen gas production by combined systems of Rhodobacter sphaeroides OU001 and Halobacterium salinarum in a photobioreactor Zabut, Baker; EI-Kahlout, Kamal; Yucel, Meral; Gündüz, Ufuk; Turker, Lemi; Eroglu, Inci (2006-09-01) Rhodobacter sphaeroides O.U.001 is a photosynthetic non-sulfur bacterium which produces hydrogen from organic compounds under anaerobic conditions. Halobacterium salinarum is an archaeon and lives under extremely halophilic conditions (4 M NaCl). H. salinarum contains a retinal protein bacteriorhodopsin in its purple membrane which acts as a light-driven proton pump. In this study the Rhodobacter sphaeroides O.U.001 culture was combined with different amounts of packed cells of H. salinarum S9 or isolated p...
Photoproduction of hydrogen from sugar refinery wastewater by Rhodobacter sphaeroides OU 001 Yetis, M; Gündüz, Ufuk; Eroglu, I; Yucel, M; Turker, L (2000-11-01) Pretreated sugar refinery wastewater (SRWW) was used for the production of hydrogen by Rhodobacter sphaeroides O.U.001 in a 0.4 1 column photobioreactor. Hydrogen was produced at a rate of 0.001 1 hydrogen/h/l culture in 20% dilution of SRWW. To adjust the carbon concentration to 70 mM and nitrogen concentration to 2 mM, sucrose or l-malic acid was added as carbon source and sodium glutamate was added as nitrogen source to the 20% dilution of SRWW. By these adjustments, hydrogen production rate was increase...
Biological hydrogen production from olive mill wastewater and its applications to bioremediation Eroğlu, Ela; Eroğlu, İnci; Department of Chemical Engineering (2006) Hydrogen production by photosynthetic bacteria occurs under illumination in the presence of anaerobic atmosphere from the breakdown of organic substrates, which is known as photofermentation. In this study, single-stage and two-stage process development were investigated for photofermentative hydrogen production from olive mill wastewater by Rhodobacter sphaeroides O.U.001 within indoor and outdoor photobioreactors. It was proven that diluted olive mill wastewater (OMW) could be utilized for photobiological...

Citation Formats

B. Uyar, İ. EROĞLU, M. Yucel, U. Gündüz, and L. Turker, “Effect of light intensity, wavelength and illumination protocol on hydrogen production in photobioreactors,” INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, pp. 4670–4677, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32258.