Suppression of STAT5A increases chemotherapeutic sensitivity in imatinib-resistant and imatinib-sensitive K562 cells

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Date

2010-10-01

Author

KOSOVA, BUKET
Tezcanli, Burcin
Ekiz, Huseyin Atakan
Cakir, Zeynep
SELVİ GÜNEL, NUR
Dalmizrak, Aysegul
Kartal, Melis
Gündüz, Ufuk
BARAN, YUSUF

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STAT proteins are cytoplasmic transcription factors that are involved in the regulation of numerous cellular activities such as cell growth, differentiation, and survival. In this study, we aimed to identify the expression pattern of STAT genes in imatinib-sensitive and -resistant K562 cells, and further, to reveal the effects of STAT5A siRNA knockdown on cell growth and apoptosis induction. The XTT cell proliferation assay showed that both sensitive and resistant K562 cells were sensitized to imatinib upon transfection with STAT5A siRNA. Caspase-3 enzyme activity was increased significantly in both cells. These results may open up new opportunities to overcome chemotherapeutic resistance in leukemia.

Subject Keywords

Chemotherapeutic resistance, Chronic myeloid leukemia, Reversal of resistance, Imatinib, STAT5A, SiRNA knockdown

URI

https://hdl.handle.net/11511/30851

Journal

LEUKEMIA & LYMPHOMA

DOI

https://doi.org/10.3109/10428194.2010.507830

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

B. KOSOVA et al., “Suppression of STAT5A increases chemotherapeutic sensitivity in imatinib-resistant and imatinib-sensitive K562 cells,” LEUKEMIA & LYMPHOMA, pp. 1895–1901, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30851.