Biohydrogen production by Rhodobacter capsulatus in solar tubular photobioreactor on thick juice dark fermenter effluent

Boran, Efe
Ozgur, Ebru
Yucel, Meral
Gündüz, Ufuk
Eroglu, Inci
The purpose of this study was to operate a pilot scale solar tubular photobioreactor (90 L) for photofermentative hydrogen production from thick juice dark fermenter effluent (DEE) by photosynthetic purple-non-sulfur bacterium, Rhodobacter capsulatus. The tubular photobioreactor was equipped with internal cooling tubes for effective temperature control, which is essential for stable, long-term operation. The photobioreactor was operated in fed-batch mode throughout September 2009 in Ankara, Turkey. R. capsulatus had a rapid growth with a specific growth rate of 0.025 h(-1) in the exponential phase. The growth was modeled and the parameters were compared with the results obtained in artificial medium. An exponential relationship was found between daily specific growth rates during fedbatch operation and daily total light energy received. The average molar productivity calculated according to daylight time was 0.15 mol H-2/(m(3) h). The yield obtained was 0.4 mol H-2/mol acetic acid consumed. The hydrogen yield factor was related to the daily total light energy received. Acetic acid utilization for different metabolic pathways was investigated. Maximum COD removal efficiency throughout the operation was calculated as 68%. This study showed that sequential operation of dark and photofermentation is a promising route to produce biological hydrogen from thick juice.


Biohydrogen production by Rhodobacter capsulatus Hup(-) mutant in pilot solar tubular photobioreactor
Boran, Efe; Ozgur, Ebru; Yucel, Meral; Gündüz, Ufuk; EROĞLU, İNCİ (2012-11-01)
In this study, a pilot solar tubular photobioreactor was successfully implemented for fed batch operation in outdoor conditions for photofermentative hydrogen production with Rhodobacter capsulatus (Hup(-)) mutant. The bacteria had a rapid growth with a specific growth rate of 0.052 h(-1) in the batch exponential phase and cell dry weight remained in the range of 1-1.5 g/L throughout the fed batch operation. The feeding strategy was to keep acetic acid concentration in the photobioreactor at the range of 20...
Photofermentative hydrogen production using dark fermentation effluent of sugar beet thick juice in outdoor conditions
Ozkan, Endam; Uyar, Basar; Ozgur, Ebru; Yucel, Meral; Eroglu, Inci; Gündüz, Ufuk (2012-01-01)
In the present study, photofermentative hydrogen production on thermophilic dark fermentation effluent (DFE) of sugar beet thick juice was investigated in a solar fed-batch panel photobioreactor (PBR) using Rhodobacter capsulatus YO3 (hup(-)) during summer 2009 in Ankara, Turkey. The DFE was obtained by continuous dark fermentation of sugar beet thick juice by extreme thermophile Caldicellulosiruptor saccharolyticus and it contains acetate (125 mM) and NH4+ (7.7 mM) as the main carbon and nitrogen sources, ...
Photofermentative hydrogen production from molasses in tubular photobioreactor with pH control
Oflaz, Fatma Betül; Koku, Harun; Department of Chemical Engineering (2019)
Biological hydrogen production has the potential to supply hydrogen from various wastes as feedstock and operation under ambient conditions. In order to obtain cost effective production, photobioreactors (PBRs) that can operate for long durations while utilizing waste are necessary. Two primary issues limiting the duration are decrease in pH and the non-optimal C/N ratio. The main aim of this study was to construct and operate a pH control system for a pilot scale photobioreactor (20 L) to achieve prolonged...
Biohydrogen production by Rhodobacter capsulatus on acetate at fluctuating temperatures
Ozgur, Ebru; Uyar, Basar; Ozturk, Yavuz; Yucel, Meral; Gündüz, Ufuk; Eroglu, Inci (2010-03-01)
Hydrogen is a clean energy alternative to fossil fuels. Photosynthetic bacteria produce hydrogen from organic compounds under anaerobic, nitrogen-limiting conditions through a light-dependent electron transfer process. In this study, the hydrogen production efficiency of phototrophic bacteria, Rhodobacter capsulatus and its Hup mutant strain (an uptake hydrogenase deleted strain) were tested on different initial acetate concentrations at fluctuating temperatures with indoor and outdoor photobioreactors. Ace...
Photoproduction of hydrogen by Rhodobacter capsulatus from thermophilic fermentation effluent
Uyar, Basar; Schumacher, Matthias; Gebicki, Jakub; Modigell, Michael (2009-08-01)
Rhodobacter capsulatus was used for the phototrophic hydrogen production on effluent solution derived from the thermophilic fermentation of Miscanthus hydrolysate by Thermotoga neapolitana. Pretreatments such as centrifugation, dilution, buffer addition, pH adjustment and sterilization were suggested for the effluent before being fed to the photofermentation. Batch-wise experiments showed that R. capsulatus grows and produces hydrogen on the pretreated effluent solution. Moreover, it was found that the hydr...
Citation Formats
E. Boran, E. Ozgur, M. Yucel, U. Gündüz, and I. Eroglu, “Biohydrogen production by Rhodobacter capsulatus in solar tubular photobioreactor on thick juice dark fermenter effluent,” JOURNAL OF CLEANER PRODUCTION, pp. 150–157, 2012, Accessed: 00, 2020. [Online]. Available: