Photofermentative hydrogen production using dark fermentation effluent of sugar beet thick juice in outdoor conditions

Date

2012-01-01

Author

Ozkan, Endam
Uyar, Basar
Ozgur, Ebru
Yucel, Meral
Eroglu, Inci
Gündüz, Ufuk

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

181
views

0
downloads

In the present study, photofermentative hydrogen production on thermophilic dark fermentation effluent (DFE) of sugar beet thick juice was investigated in a solar fed-batch panel photobioreactor (PBR) using Rhodobacter capsulatus YO3 (hup(-)) during summer 2009 in Ankara, Turkey. The DFE was obtained by continuous dark fermentation of sugar beet thick juice by extreme thermophile Caldicellulosiruptor saccharolyticus and it contains acetate (125 mM) and NH4+ (7.7 mM) as the main carbon and nitrogen sources, respectively. The photofermentation process was done in a 4 L plexiglas panel PBR which was daily fed at a rate of 10% of the PBR volume. The DFE was diluted 3 times to adjust the acetate concentration to approximately 40 mM and supplemented with potassium phosphate buffer, Fe and Mo. In order to control the temperature, cooling was provided by recirculating chilled water through a tubing inside the reactor. Hydrogen productivity of 1.12 mmol/L-c/h and molar yield of 77% of theoretical maximum over consumed substrate were attained over 15 days of operation. The results indicated that Rb. capsulatus YO3 could effectively utilize the DFE of sugar beet thick juice for growth and hydrogen production, therefore facilitating the integration of the dark and photo-fermentation processes for sustainable biohydrogen production. Copyright (C) 2011, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.

Subject Keywords

Sugar beet thick juice, Panel photobioreactor, Photofermentation, Biohydrogen, Rhodobacter capsulatus

URI

https://hdl.handle.net/11511/31908

Journal

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY

DOI

https://doi.org/10.1016/j.ijhydene.2011.06.035

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Biohydrogen production by Rhodobacter capsulatus in solar tubular photobioreactor on thick juice dark fermenter effluent Boran, Efe; Ozgur, Ebru; Yucel, Meral; Gündüz, Ufuk; Eroglu, Inci (2012-08-01) The purpose of this study was to operate a pilot scale solar tubular photobioreactor (90 L) for photofermentative hydrogen production from thick juice dark fermenter effluent (DEE) by photosynthetic purple-non-sulfur bacterium, Rhodobacter capsulatus. The tubular photobioreactor was equipped with internal cooling tubes for effective temperature control, which is essential for stable, long-term operation. The photobioreactor was operated in fed-batch mode throughout September 2009 in Ankara, Turkey. R. capsu...
Photoproduction of hydrogen by Rhodobacter capsulatus from thermophilic fermentation effluent Uyar, Basar; Schumacher, Matthias; Gebicki, Jakub; Modigell, Michael (2009-08-01) Rhodobacter capsulatus was used for the phototrophic hydrogen production on effluent solution derived from the thermophilic fermentation of Miscanthus hydrolysate by Thermotoga neapolitana. Pretreatments such as centrifugation, dilution, buffer addition, pH adjustment and sterilization were suggested for the effluent before being fed to the photofermentation. Batch-wise experiments showed that R. capsulatus grows and produces hydrogen on the pretreated effluent solution. Moreover, it was found that the hydr...
Photobiological hydrogen production by using olive mill wastewater as a sole substrate source Eroglu, E; Gündüz, Ufuk; Yucel, M; Turker, L; Eroglu, I (2004-02-01) In the present work olive mill wastewater (OMW) collected from West Anatolia-Turkey during 2001, containing 36.02 g carbon, 5.26 g hydrogen, and 0.96 g nitrogen in 100 g suspended solid was used as a sole substrate for the production of hydrogen gas by Rhodobacter sphaeroides O.U.001 in 400 ml glass, column-photobioreactors. Hydrogen production studies on diluted-OMW were investigated in the range of 20% (v/v) and 1% (v/v) OMW containing media. Below 5% OMW containing media, bacterial growth rate fitted wel...
Photoproduction of hydrogen from sugar refinery wastewater by Rhodobacter sphaeroides OU 001 Yetis, M; Gündüz, Ufuk; Eroglu, I; Yucel, M; Turker, L (2000-11-01) Pretreated sugar refinery wastewater (SRWW) was used for the production of hydrogen by Rhodobacter sphaeroides O.U.001 in a 0.4 1 column photobioreactor. Hydrogen was produced at a rate of 0.001 1 hydrogen/h/l culture in 20% dilution of SRWW. To adjust the carbon concentration to 70 mM and nitrogen concentration to 2 mM, sucrose or l-malic acid was added as carbon source and sodium glutamate was added as nitrogen source to the 20% dilution of SRWW. By these adjustments, hydrogen production rate was increase...
Biohydrogen production by Rhodobacter capsulatus Hup(-) mutant in pilot solar tubular photobioreactor Boran, Efe; Ozgur, Ebru; Yucel, Meral; Gündüz, Ufuk; EROĞLU, İNCİ (2012-11-01) In this study, a pilot solar tubular photobioreactor was successfully implemented for fed batch operation in outdoor conditions for photofermentative hydrogen production with Rhodobacter capsulatus (Hup(-)) mutant. The bacteria had a rapid growth with a specific growth rate of 0.052 h(-1) in the batch exponential phase and cell dry weight remained in the range of 1-1.5 g/L throughout the fed batch operation. The feeding strategy was to keep acetic acid concentration in the photobioreactor at the range of 20...

Citation Formats

E. Ozkan, B. Uyar, E. Ozgur, M. Yucel, I. Eroglu, and U. Gündüz, “Photofermentative hydrogen production using dark fermentation effluent of sugar beet thick juice in outdoor conditions,” INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, pp. 2044–2049, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31908.