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MATRIX ENTRAPMENT OF GLUCOSE-OXIDASE BY GAMMA-IRRADIATION
Date
1992-01-01
Author
GURSEL, I
Hasırcı, Vasıf Nejat
Metadata
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In this study, matrix entrapment of the enzyme glucose oxidase was achieved through gamma-irradiation of monomers N-vinyl pyrrolidone, 2-hydroxyethyl methacrylate and their mixture. To test the effect of radiation on entrapment efficiency, retention of activities and properties of the system, duration and temperature were varied. The reusability of the resultant products was tested. It was generally found that inclusion of the hydrophilic monomer N-vinyl pyrrolidone into the matrix increased the water content, entrapment efficiency and enzyme activity. Gamma-irradiation did not have a detrimental effect on the activity of pure enzyme when exposure was at -196-degrees-C, but this radioresistance was less at higher temperatures. Infrared spectroscopy indicated that there was no chemical binding between the matrix and the enzyme. Repeated use studies revealed a gradual loss of activity in all of the samples tested for 10 runs.
Subject Keywords
Immobilization
,
Enzymes
,
Irradiation
URI
https://hdl.handle.net/11511/31019
Journal
BIOMATERIALS
DOI
https://doi.org/10.1016/0142-9612(92)90063-t
Collections
Graduate School of Natural and Applied Sciences, Article
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I. GURSEL and V. N. Hasırcı, “MATRIX ENTRAPMENT OF GLUCOSE-OXIDASE BY GAMMA-IRRADIATION,”
BIOMATERIALS
, pp. 150–155, 1992, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31019.