Immobilization of invertase and glucose oxidase in conducting H-type polysiloxane/polypyrrole block copolymers

2003-01-01
Gursel, A
Alkan, S
Toppare, Levent Kamil
Yagci, Y
In this study, immobilizations of enzymes, invertase and glucose oxidase, were achieved in conducting copolymers of N-pyrrolyl terminated polydimethylsiloxane/polypyrrole (PDMS/PPy) matrices via electrochemical polymerization. The kinetic parameters, v(max) (maximum reaction rate) and K-m (substrate affinity), of both free and immobilized enzymes were determined. The effect of supporting electrolytes, p-toluene sulfonic acid and sodium dodecyl sulfate, on enzyme activity and film morphologies was examined. The optimum temperatures and operational stabilities of immobilized enzymes were determined. PDMS/PPy copolymer matrix was found to exhibit significantly enhanced properties compare to pristine polypyrrole in terms of relative enzyme activities, kinetic parameters and operational stabilities. (C) 2003 Published by Elsevier B.V.
REACTIVE & FUNCTIONAL POLYMERS

Suggestions

Immobilization of invertase and glucose oxidase in conducting copolymers of thiophene functionalized poly(vinyl alcohol) with pyrrole
Sahmetlioglu, E; Yuruk, H; Toppare, Levent Kamil; Cianga, I; Yagci, Y (Elsevier BV, 2006-03-01)
In this study, immobilizations of invertase and glucose oxidase were achieved in conducting thiophene functionalized copolymers of vinyl alcohol with thiophene side groups and pyrrole (PVATh/PPy) via electrochemical polymerization. The kinetic parameters, V-max (maximum reaction rate) and K-m (substrate affinity), of both free and immobilized enzymes were determined. The effect of supporting electrolytes, p-toluene sulfonic acid and sodium dodecyl sulfate, on the enzyme activity and film morphologies was ex...
Immobilization of invertase and glucose oxidase in poly 2-methylbutyl-2-(3-thienyl) acetate/polypyrrole matrices
Isik, S; Alkan, S; Toppare, Levent Kamil; Cianga, I; Yagci, Y (Elsevier BV, 2003-12-01)
Immobilization of invertase and glucose oxidase in conducting polypyrrole and copolymers of poly 2-methylbutyl-2-(3-thienyl) acetate with pyrrole were achieved via electrochemical method. Sodium dodecyl sulphate was found to be the most suitable supporting electrolyte. Maximum reaction rate, Michaelis-Menten constant and optimum temperatures were determined for native and immobilized enzymes. Storage and operational stabilities of enzyme electrodes were also investigated.
Immobilization of Tyrosinase in Poly(2-thiophen-3-yl-alkyl ester) Derivatives
ÇAMURLU, PINAR; Kayahan, Senem; Toppare, Levent Kamil (Informa UK Limited, 2008-01-01)
In this study, construction of novel biosensors for the determination of phenolic compound was performed via immobilization of tyrosinase during the electrochemical synthesis of conducting block copolymers of 2-thiophen-3-yl-alkyl ester derivatives with 3,4-ethylenedioxythiophene and synthesis of poly(3,4-ethylenedioxythiophene) (PEDOT). The resultant biosensors were characterized in terms of their maximum reaction rates, Michaelis-Menten constants (Km), temperature and pH stabilities. All the copolymer mat...
Immobilization of invertase on a conducting polymer of 1-(4-nitrophenyl)-2,5-di(2-thienyl)-1H-pyrrole
Tuncagil, Sevinc; Kiralp, Senem; Varls, Serhat; Toppare, Levent Kamil (Elsevier BV, 2008-03-01)
In this study, immobilization of invertase was achieved on a conducting polymer of 1-(4-nitrophenyl)-2,5-di(2-thienyl)-1H-pyrrole) (SNS (NO2)) via electrochemical polymerization. Kinetic parameters, maximum reaction rate (V-max) and substrate affinity (K-m), optimum temperature and pH, operational and storage stabilities of immobilized enzyme were determined.
Immobilization of invertase in conducting polypyrrole/PMMA-co-PMTM graft copolymers
Yildiz, HB; Kiralp, S; Toppare, Levent Kamil; Yagci, Y (Wiley, 2005-04-15)
In this study, invertase was immobilized in copolymer electrodes constructed. Three different types of polymethyl methacrylate-co-polymethyl thienyl methacrylate matrices were used to obtain copolymers that were characterized by FT-IR spectroscopy. Immobilization of enzymes was carried out by the entrapment of the enzyme in conducting polymer matrices during electrochemical polymerization of pyrrole through thiophene moieties of polymers. Immobilization of the enzyme was achieved by application of 1.0 V con...
Citation Formats
A. Gursel, S. Alkan, L. K. Toppare, and Y. Yagci, “Immobilization of invertase and glucose oxidase in conducting H-type polysiloxane/polypyrrole block copolymers,” REACTIVE & FUNCTIONAL POLYMERS, pp. 57–65, 2003, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48649.