Immobilization of invertase and glucose oxidase in conducting H-type polysiloxane/polypyrrole block copolymers

Date

2003-01-01

Author

Gursel, A
Alkan, S
Toppare, Levent Kamil
Yagci, Y

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

313
views

0
downloads

In this study, immobilizations of enzymes, invertase and glucose oxidase, were achieved in conducting copolymers of N-pyrrolyl terminated polydimethylsiloxane/polypyrrole (PDMS/PPy) matrices via electrochemical polymerization. The kinetic parameters, v(max) (maximum reaction rate) and K-m (substrate affinity), of both free and immobilized enzymes were determined. The effect of supporting electrolytes, p-toluene sulfonic acid and sodium dodecyl sulfate, on enzyme activity and film morphologies was examined. The optimum temperatures and operational stabilities of immobilized enzymes were determined. PDMS/PPy copolymer matrix was found to exhibit significantly enhanced properties compare to pristine polypyrrole in terms of relative enzyme activities, kinetic parameters and operational stabilities. (C) 2003 Published by Elsevier B.V.

Subject Keywords

Materials Chemistry, Biochemistry, General Chemistry, General Chemical Engineering, Polymers and Plastics, Environmental Chemistry

URI

https://hdl.handle.net/11511/48649

Journal

REACTIVE & FUNCTIONAL POLYMERS

DOI

https://doi.org/10.1016/j.reactfunctpolym.2003.07.004

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Immobilization of invertase and glucose oxidase in conducting copolymers of thiophene functionalized poly(vinyl alcohol) with pyrrole Sahmetlioglu, E; Yuruk, H; Toppare, Levent Kamil; Cianga, I; Yagci, Y (Elsevier BV, 2006-03-01) In this study, immobilizations of invertase and glucose oxidase were achieved in conducting thiophene functionalized copolymers of vinyl alcohol with thiophene side groups and pyrrole (PVATh/PPy) via electrochemical polymerization. The kinetic parameters, V-max (maximum reaction rate) and K-m (substrate affinity), of both free and immobilized enzymes were determined. The effect of supporting electrolytes, p-toluene sulfonic acid and sodium dodecyl sulfate, on the enzyme activity and film morphologies was ex...
Immobilization of invertase on a conducting polymer of 1-(4-nitrophenyl)-2,5-di(2-thienyl)-1H-pyrrole Tuncagil, Sevinc; Kiralp, Senem; Varls, Serhat; Toppare, Levent Kamil (Elsevier BV, 2008-03-01) In this study, immobilization of invertase was achieved on a conducting polymer of 1-(4-nitrophenyl)-2,5-di(2-thienyl)-1H-pyrrole) (SNS (NO2)) via electrochemical polymerization. Kinetic parameters, maximum reaction rate (V-max) and substrate affinity (K-m), optimum temperature and pH, operational and storage stabilities of immobilized enzyme were determined.
Immobilization of invertase in conducting polypyrrole/PMMA-co-PMTM graft copolymers Yildiz, HB; Kiralp, S; Toppare, Levent Kamil; Yagci, Y (Wiley, 2005-04-15) In this study, invertase was immobilized in copolymer electrodes constructed. Three different types of polymethyl methacrylate-co-polymethyl thienyl methacrylate matrices were used to obtain copolymers that were characterized by FT-IR spectroscopy. Immobilization of enzymes was carried out by the entrapment of the enzyme in conducting polymer matrices during electrochemical polymerization of pyrrole through thiophene moieties of polymers. Immobilization of the enzyme was achieved by application of 1.0 V con...
Voltammetric methods of reboxetine analysis and the mechanism of its electrode reactions Altunöz Erdoğan, Deniz; Erk, Nevin; Kilic, Esma (Walter de Gruyter GmbH, 2013-05-01) Reboxetine (RBX) electrochemical redox behavior at hanging mercury drop (HMDE) and glassy carbon electrodes (GCE) was studied in various pH Britton-Robinson universal buffers using cyclic voltammetry and square-wave voltammetry. RBX was reduced at the HMDE and oxidized at the GCE with reversible adsorption controlled and irreversible diffusion controlled processes respectively. The anodic peak is due to the amine and the cathodic peak may correspond to oxygen protonation. An oxidation reaction mechanism is ...
Synthesis, Characterization and Optoelectrochemical Properties of Poly(2,5-di(thiophen-2-yl)-1-(4-(thiophen-3-yl)phenyl)-1H-pyrrole-co-EDOT) Turac, Ersen; Sahmetlioglu, Ertugrul; Toppare, Levent Kamil; Yuruk, Huseyin (Informa UK Limited, 2010-01-01) A new polythiophene derivative was synthesized by electrochemical oxidative polymerization of 2,5-di( thiophen-2-yl)-1-(4-(thiophen-3-yl) phenyl)-1H-pyrrole (TTPP). The structure of the monomer was evaluated by H-1-NMR and FT-IR. The polymer (P(TTPP)) and its co-polymer with 3,4-ethylenedioxythiophene (P(TTPP-co-EDOT)) were synthesized via potentiostatic electrochemical polymerization. The resulting polymers were characterized by cyclic voltammetry (CV), FT-IR, SEM and UV-Vis spectroscopy, and conductivity ...

Citation Formats

A. Gursel, S. Alkan, L. K. Toppare, and Y. Yagci, “Immobilization of invertase and glucose oxidase in conducting H-type polysiloxane/polypyrrole block copolymers,” REACTIVE & FUNCTIONAL POLYMERS, pp. 57–65, 2003, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48649.