PCL-TCP wet spun scaffolds carrying antibiotic-loaded microspheres for bone tissue engineering

Date

2018-01-01

Author

Malikmammadov, Elbay
Endoğan Tanır, Tuğba
Kızıltay, Aysel
Hasırcı, Vasıf Nejat
Hasırcı, Nesrin

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Scaffolds produced for tissue engineering applications are proven to be promising alternatives to be used in healing and regeneration of injured tissues and organs. In this study, porous and fibrous poly(epsilon-caprolactone) (PCL) scaffolds were prepared by wet spinning technique and modified by addition of tricalcium phosphate (TCP) and by immobilizing gelatin onto fibers. Meanwhile, gelatin microspheres carrying Ceftriaxone sodium (CS), a model antibiotic, were added onto the scaffolds and antimicrobial activity of CS was investigated against Escherichia coli (E. coli), a model gram-negative bacterium. TCP and gelatin were added to enhance mechanical properties while directing the scaffold towards osteogenic infrastructure and to increase hydrophilicity by activating cell attachment via protein molecules, respectively. Modifications with TCP and gelatin enhanced thecompression modulus byabout 70%, and attachment of Saos-2 cells by60%, respectively. Release of the antibiotic demonstrated effective antimicrobial activity against E. coli. The bioactive scaffolds wereshown to be good candidates for bone tissue engineering applications.

Subject Keywords

Bone tissue engineering , Anti-bacterial, Wet spinning, PCL, Scaffold, Poly(epsilon-caprolactone), Biofunctionalization, Gelatin immobilization

URI

https://hdl.handle.net/11511/31109

Journal

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION

DOI

https://doi.org/10.1080/09205063.2017.1354671

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

E. Malikmammadov, T. Endoğan Tanır, A. Kızıltay, V. N. Hasırcı, and N. Hasırcı, “PCL-TCP wet spun scaffolds carrying antibiotic-loaded microspheres for bone tissue engineering,” JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, pp. 805–824, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31109.