Heparin/Chitosan/Alginate Complex Scaffolds as Wound Dressings: Characterization and Antibacterial Study Against Staphylococcus epidermidis

Sezer, Umran Aydemir
Kara, Filiz
Hasırcı, Nesrin
Infected wounds are global healthcare problems since they can lead to dysfunctioning in organs and even result in death in severe cases. In this study, antibacterial wound dressings were prepared to prevent possible infection at the damaged host region. For this purpose, three natural polymers namely heparin, chitosan and alginate were used as anionic, cationic and sulfated polysaccharides, respectively, due to their versatile properties as being nontoxic, biocompatible, biodegradable and antibacterial. Heparin/chitosan/alginate based complex polyelectrolyte scaffolds containing different amounts of heparin were prepared by freeze drying technique. The effects of calcium chloride, which is used as crosslinker for alginate, on the stability of scaffolds were tested. Presence of functional groups and morphological structures of the scaffolds were examined in detail by using FTIR, XPS and SEM. Antibacterial activities against Staphylococcus epidermidis (S. epidermidis) were investigated under in vitro conditions. Presence and the amount of heparin in the scaffolds significantly enhanced the antibacterial activities. The results demonstrated that heparin/chitosan/alginate polyelectrolyte scaffolds have very high potential to be used as therapeutic wound dressings.


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There is a high need for rapid healing in the treatment of severe burns, trauma, diabetic, decubitus ulcers and other conditions where a great damage of the tissue is exist. In those cases, wound should be covered with a dressing which replaces the functions of the natural skin by protecting the loss of body fluid and proteins, preventing bacterial invasion, improving and stimulating the healing process by providing a support for the proliferating cells
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Catheter-associated infections, most of which are caused by microbial biofilms, are still a serious issue in healthcare and are associated with significant morbidity, mortality, and excessive medical costs. Currently, the use of nanostructured materials, especially materials with nano featured topographies, which have more surface area, altered surface energy, enhanced select protein adsorption, and selectively increased desirable cell functions while simultaneously decreasing competitive cell functions, se...
Nakipoğlu, Mustafa; Tezcaner, Ayşen; Özen, Can; Department of Biotechnology (2023-1-18)
Despite the advancement of modern technology, wound care is still based on traditional methods such as suture, staple and gauze around the world. Although, these methods provide affordable treatment for various types of wounds, they fall short in easy and effective treatment to facilitate healing process. Enabling a quicker and more convenient wound healing necessitates a comprehensive approach to wound dressing development to include simple yet multifunctional and versatile designs. In this work, we focuse...
Natural origin bilayer pullulan-PHBV scaffold for wound healing applications
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Skin tissue loss that occurs by injury and diseases can turn into chronic wounds as a result of complications alongside infection. Chronic wounds fail to heal by themselves and need advanced treatments like engineered wound dressings and regenerative scaffolds. In this study, a novel, natural origin, bilayer electrospun scaffold was produced from pullulan (PUL) and poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) biopolymers. PHBV production by Cupriavidus necator bacterial strain was optimized and produced ...
Citation Formats
E. A. AKSOY, U. A. Sezer, F. Kara, and N. Hasırcı, “Heparin/Chitosan/Alginate Complex Scaffolds as Wound Dressings: Characterization and Antibacterial Study Against Staphylococcus epidermidis,” JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING, pp. 104–113, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31177.