Polyhydroxybutyrate-Coated Magnetic Nanoparticles for Doxorubicin Delivery: Cytotoxic Effect Against Doxorubicin-Resistant Breast Cancer Cell Line

Date

2014-11-01

Author

Yalcin, Serap
Unsoy, Gozde
Mutlu, Pelin
Khodadust, Rouhollah
Gündüz, Ufuk

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In this study, polyhydroxybutyrate (PHB)-coated magnetic nanoparticles (MNPs) were prepared by coprecipitation of iron salts (Fe2+ and Fe3+) by ammonium hydroxide. Characterizations of PHB-coated MNPs were performed by Fourier transform infrared spectroscopy, x-ray diffraction, dynamic light scattering, thermal gravimetric analysis, vibrating sample magnetometry, and transmission electron microscopy analyses. Doxorubicin was loaded onto PHB-MNPs, and the release efficiencies at different pHs were studied under in vitro conditions. The most efficient drug loading concentration was found about 87% at room temperature in phosphate-buffered saline (pH 7.2). The drug-loaded MNPs were stable up to 2 months in neutral pH for mimicking physiological conditions. The drug release studies were performed with acetate buffer (pH 4.5) that mimics endosomal pH. Doxorubicin (60%) released from PHB-MNPs within 65 hours. Doxorubicin-loaded PHB-MNPs were about 2.5-fold more cytotoxic as compared with free drug on resistant Michigan Cancer Foundation-7 (human breast adenocarcinoma, MCF-7) cell line (1 mu M doxorubicin) in vitro. Therefore, doxorubicin-loaded PHB-MNPs lead to overcome the drug resistance.

Subject Keywords

PHB, Doxorubicin, Magnetic Nanoparticles, Targeted Drug Delivery, Drug Resistance

URI

https://hdl.handle.net/11511/31528

Journal

AMERICAN JOURNAL OF THERAPEUTICS

DOI

https://doi.org/10.1097/mjt.0000000000000066

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

S. Yalcin, G. Unsoy, P. Mutlu, R. Khodadust, and U. Gündüz, “Polyhydroxybutyrate-Coated Magnetic Nanoparticles for Doxorubicin Delivery: Cytotoxic Effect Against Doxorubicin-Resistant Breast Cancer Cell Line,” AMERICAN JOURNAL OF THERAPEUTICS, pp. 453–461, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31528.