LANTHANUM DOPED DICALCIUM PHOSPHATE MIXED WITH GRAPHENE OXIDE BONE CEMENTS AS A POTENTIAL MATERIAL IN BIOMEDICAL APPLICATIONS

Download

ali motameni 1420637.pdf

Date

2021-9-06

Author

Motameni, Ali

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

403
views

265
downloads

In this thesis, mesoporous particles of β-tricalcium phosphate (βTCP, βCa3(PO4)2) and lanthanum (La) doped βTCP were synthesized by wet precipitation method attached with a microwave refluxing system. Pure dicalcium phosphate (DCP) and La modified dicalcium phosphate (La-DCP) bone cements were prepared based on acid/base reaction between βTCP (or La- βTCP) and monocalcium phosphate monohydrate (MCPM) in the presence of water. DCP bone cements were also mixed with 1.5-3.5wt.% of graphene oxide (GO). The obtained materials were characterized by XRD, FTIR, SEM, ICP-OES, BET, and helium pycnometer. The loading /releasing analysis of proteins on/from βTCP and La- βTCP disks surfaces were evaluated using Fetal bovine serum (FBS) proteins. In vitro cell culture studies were performed with the human osteosarcoma cell line (Saos-2). Upon incorporating La3+ ions, expansion in lattice parameters of βTCP crystal was observed along with inhibition of growth rate. βTCP and La- βTCP materials were mesoporous in nature. Pore diameter and pore volume were expanded with the incorporation of La3+ ions. The outcomes confirmed that all La-βTCP materials were cytocompatible, and strict dose dependent effect of La3+ ions was observed on cell viability and alkaline phosphatase (ALP) activity. In DCP bone cements, the pure phase of brushite transformed into monetite with minimum addition of La3+ ions (0.090 mole), and the plate-like crystals of brushite turned into spheroid particles. The results of in vitro experiments on proliferation, adhesion, and osteogenic differentiation of Saos-2 cells indicated that the addition of 0.225 mole of La3+ ions promoted these properties compared to pure DCP. With the addition of GO to DCP bone cements, the mechanical properties of 2La-DCP cements improved. As the GO/2La-DCP bone cements were biocompatible, proliferation and differentiation properties of cells were significantly improved with the addition of GO.

Subject Keywords

β-tricalcium phosphate, Lanthanum, Dicalcium Phosphate, Graphene Oxide

URI

https://hdl.handle.net/11511/94636

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Polybenzimidazole based nanocomposite membranes with enhanced proton conductivity for high temperature PEM fuel cells Ozdemir, Yagmur; Uregen, Nurhan; DEVRİM, YILSER (2017-01-26) In this study, phosphoric acid doped PBI nanocomposite membranes were prepared by dispersion of various amounts of inorganic nanoparticles in PBI polymer followed by phosphoric acid (H3PO4) doping for high temperature proton exchange membrane fuel cells (HT-PEMFC). All of the PBI composite membranes were cast from the same FBI polymer with the same molecular weight. Titanium dioxide (TiO2), silicon dioxide (SiO2) and inorganic proton conductor zirconium phosphate (ZrP) were used as inorganic fillers. The PB...
Thermoluminescence properties of ZnO nanoparticles in the temperature range 10-300 K IŞIK, MEHMET; YILDIRIM, TACETTİN; Hasanlı, Nızamı (2016-04-01) Low-temperature thermoluminescence (TL) properties of ZnO nanoparticles grown by sol-gel method were investigated in the 10-300 K temperature range. TL glow curve obtained at 0.2 K/s constant heating rate exhibited one broad peak around 83 K. The observed peak was analyzed using curve fitting method to determine the activation energies of trapping center(s) responsible for glow curve. Analyses resulted in the presence of three peaks at 55, 85 and 118 K temperatures with activation energies of 12, 30 and 45 ...
Synergistic fire retardancy of colemanite, a natural hydrated calcium borate, in high-impact polystyrene containing brominated epoxy and antimony oxide Kaynak, Cevdet (2011-05-01) This study explores for the first time the synergistic fire retardant action of natural hydrated calcium borate, namely the mineral colemanite, which partially replaces antimony oxide in brominated flame retardant high-impact polystyrene compounds. Various antimony oxide to hydrated calcium borate ratios were employed keeping the brominated flame retardant additive at a constant loading level. With partial colemanite substitution for antimony oxide, lower heat release rate, total heat evolved and fire growt...
Tunable optical properties and DFT calculations of RE3+ codoped LaBO3 phosphors Yılmaz, Ayşen; Esentürk, Okan (2019-12-01) In this study, single phase DyxLa1-xBO3, DyxCeyLa1-x-yBO3, DyxEuyLa1-x-yBO3, and TbxCeyLa1-x-yBO3 phosphors were successfully synthesized through the microwave assisted solid state method with urea. Synthesized compounds were characterized by X-Ray Diffraction, Fourier Transform Infrared spectroscopy, Scanning Electron Microscope, and Fluorescence spectroscopy. Thermal stabilities of the phosphors were studied with temperature dependent fluorescence measurements. In addition, density functional theory calcu...
Polyhydroxybutyrate-Coated Magnetic Nanoparticles for Doxorubicin Delivery: Cytotoxic Effect Against Doxorubicin-Resistant Breast Cancer Cell Line Yalcin, Serap; Unsoy, Gozde; Mutlu, Pelin; Khodadust, Rouhollah; Gündüz, Ufuk (2014-11-01) In this study, polyhydroxybutyrate (PHB)-coated magnetic nanoparticles (MNPs) were prepared by coprecipitation of iron salts (Fe2+ and Fe3+) by ammonium hydroxide. Characterizations of PHB-coated MNPs were performed by Fourier transform infrared spectroscopy, x-ray diffraction, dynamic light scattering, thermal gravimetric analysis, vibrating sample magnetometry, and transmission electron microscopy analyses. Doxorubicin was loaded onto PHB-MNPs, and the release efficiencies at different pHs were studied un...

Citation Formats

A. Motameni, “LANTHANUM DOPED DICALCIUM PHOSPHATE MIXED WITH GRAPHENE OXIDE BONE CEMENTS AS A POTENTIAL MATERIAL IN BIOMEDICAL APPLICATIONS,” Ph.D. - Doctoral Program, Middle East Technical University, 2021.