Dynamic cell culturing and its application to micropatterned, elastin-like protein-modified poly(N-isopropylacrylamide) scaffolds

Date

2009-10-01

Author

Ozturk, Nihan
Girotti, Alessandra
KÖSE, GAMZE
Rodriguez-Cabello, Jose C.
Hasırcı, Vasıf Nejat

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In this study a tissue engineering scaffold was constructed from poly(N-isopropylacrylamide) (pNIPAM) to study the influence of strain on cell proliferation and differentiation. The effect of surface chemistry and topography on bone marrow mesenchymal stem cells was also investigated. Micropatterned pNIPAM films (channels with 10 mu m groove width, 2 mu m ridge width, 20 mu m depth) were prepared by photo-polymerization. The films were chemically modified by adsorption of a genetically engineered and temperature sensitive elastin-like protein (ELP). Dynamic conditions were generated by repeated temperature changes between 29 degrees C and 37 degrees C. ELP presence on the films enhanced initial cell attachment two fold (Day 1 cell number on films with ELP and without ELP were 27.6 x 10(4) and 13.2 x 10(4). respectively) but had no effect on proliferation in the long run. ELP was crucial for maintaining the cells attached on the surface in dynamic culturing (Day 7 cell numbers on the films with and without ELP were 81.4 x 10(4) and 12.1 x 10(4), respectively) and this enhanced the ability of pNIPAM films to transfer mechanical stress on the cells. Dynamic conditions improved cell proliferation (Day 21 cell numbers with dynamic and with static groups were 180.4 x 10(4) and 157.7 x 10(4), respectively) but decreased differentiation (Day 14 specific ALP values on the films of static and dynamic groups were 6.6 and 3.5 nmol/min/cell, respectively). Thus, a physically and chemically modified pNIPAM scaffold had a positive influence on the population of the scaffolds under dynamic culture conditions.

Subject Keywords

Thermoresponsive, pNIPAM, Mechanical stress, Elastin-like protein, Bone tissue engineering

URI

https://hdl.handle.net/11511/31533

Journal

BIOMATERIALS

DOI

https://doi.org/10.1016/j.biomaterials.2009.06.044

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

N. Ozturk, A. Girotti, G. KÖSE, J. C. Rodriguez-Cabello, and V. N. Hasırcı, “Dynamic cell culturing and its application to micropatterned, elastin-like protein-modified poly(N-isopropylacrylamide) scaffolds,” BIOMATERIALS, pp. 5417–5426, 2009, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31533.