Kinetics of biological hydrogen production by the photosynthetic bacterium Rhodobacter sphaeroides O.U. 001

Date

2003-04-01

Author

Koku, Harun
Gündüz, Ufuk
Türker, Lemi

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The kinetics and the effects of various parameters on hydrogen production by Rhodobacter sphaeroides O.U. 001 were investigated in a batch column photobioreactor. In particular, the effect of the inoculum age and the implementation of a light-dark cycle illumination scheme for emulating natural sunlight have been investigated in detail. The possibility of using yeast extract to replace the rather expensive vitamin mixture in the medium was also studied. The results show that hydrogen production is decreased when the initially inoculated bacteria have a high culture age. Exposure of the bacterial culture to light-dark cycles increased the amount of hydrogen compared to continuous illumination, all other parameters remaining the same. Similarly, the use of yeast extract to replace the vitamins increased the growth and hydrogen production rates, however, with a slight reduction in the total amount of gas produced and the hydrogen fraction in the evolved gas. (C) 2002 International Association for Hydrogen Energy.

Subject Keywords

Hydrogen production, Photosynthetic bacteria, Rhodobacter sphaeroides, Growth kinetics, Malate consumption

URI

https://hdl.handle.net/11511/32031

Journal

International Journal of Hydrogen Energy

DOI

https://doi.org/10.1016/s0360-3199(02)00080-0

Collections

Graduate School of Natural and Applied Sciences, Article

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Continuous hydrogen production by Rhodobacter sphaeroides O.U.001 Eroğlu, İnci; Aslan, K; Gunduz, M; Yucel, M; Turker, L (1997-06-26) This paper describes hydrogen gas production by Rhodobacter sphaeroides O.U.001 using a column photobioreactor in batch and continuous operation. The effect of substrates on the hydrogen production rate was investigated in batch-type photobioreactor experiments. Substrate concentrations (L-malic acid and sodium glutamate) were measured by using high-pressure liquid chromatography. The gas produced was analyzed by gas chromatography.
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Citation Formats

H. Koku, U. Gündüz, and L. Türker, “Kinetics of biological hydrogen production by the photosynthetic bacterium Rhodobacter sphaeroides O.U. 001,” International Journal of Hydrogen Energy, pp. 381–388, 2003, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32031.