Preparation and characterization of surface modified gamma-Fe2O3 (maghemite)-silica nanocomposites used for the purification of benzaldehyde lyase

Date

2011-08-01

Author

TURAL, Bilsen
Sopaci, S. Betul
Özkan, Necati
Demir, Ayhan Sıtkı
Volkan, Mürvet

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

182
views

0
downloads

gamma-Fe2O3 (maghemite)-silica nanocomposite particles were synthesized using a sol-gel method. The condensation products of 3-glycidoxy propyltrimethoxy silane (GPTMS) and nitrilotriacetic acid (NTA) were introduced onto the surfaces of the gamma-Fe2O3 silica nanocomposite particles and subsequently, these modified surfaces were complexed with cobalt (Co+2) metal ions. A possibility of using these surface modified gamma-Fe2O3-silica particles for the purification of 6 x histidine tagged recombinant benzaldehyde lyase (BAL, EC 4.1.2.38) based on magnetic separation was investigated. X-ray diffraction (XRD), thermal analysis, and vibrating sample magnetometry (VSM) methods were used to characterize the surface modified superparamagnetic gamma-Fe2O3 (maghemite)-silica nanoparticles. XRD (Scherer's equation) results indicate that the primary particle size of maghemite was around 11 nm. Magnetic characterization results confirmed that the gamma-Fe2O3 (maghemite)-silica nanoparticles were superparamagnetic. According to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) results, these superparamagnetic nanoparticles specifically capture 6 x His-tagged BAL from crude extract of Escherichia coli (E. coli) BL21(DE3)pLysS/BAL(HIS). This study shows that the surface modified gamma-Fe2O3 (maghemite)-silica nanoparticles are eligible for immobilized metal-ion affinity adsorption for histidine tagged recombinant proteins with its high capacity (3.16 +/- 0.4 mg/g) and selectivity.

Subject Keywords

Magnetic materials, Nanostructures, Surfaces, X-ray diffraction, Magnetic properties

URI

https://hdl.handle.net/11511/31755

Journal

JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS

DOI

https://doi.org/10.1016/j.jpcs.2011.05.010

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Modelling of Advective Transport and Biogeochemistry at a Shallow Hydrothermal Vent System Bartlett, Stuart; Giovannelli, Donato; Yücel, Mustafa (2017-08-18) Inthisworkwepresentthefirstinaseriesofmodelresultsaimedatelucidatingthecomplexcouplingsbetweenfluidflow,heattransport,advectivetransportofsolutes,andmicrobialmatgrowthatashallowhydrothermalvent.WeareprimarilyinterestedintheventsystemsofMilos,Greece,wherebiofilmcommunitiesarespatiallysegregatedintodistinctdomains,eachwithaverydifferentspeciescomposition[1].Thelocationsofthesedomainsvarystronglywiththesandbedtopography.Inparticular,therearesharpspeciescompositiondifferencesbetweenthewindwardsideand the leewar...
Measurement of pseudorapidity distributions of charged particles in proton-proton collisions at root s=8 TeV by the CMS and TOTEM experiments Chatrchyan, S.; et. al. (Springer Science and Business Media LLC, 2014-10-01) Pseudorapidity (eta) distributions of charged particles produced in proton-proton collisions at a centre-of-mass energy of 8 TeV are measured in the ranges vertical bar eta vertical bar < 2.2 and 5.3 < vertical bar eta vertical bar < 6.4 covered by the CMS and TOTEM detectors, respectively. The data correspond to an integrated luminosity of L = 45 mu b(-1). Measurements are presented for three event categories. The most inclusive category is sensitive to 91-96 % of the total inelastic proton-proton cross se...
Synthesis and characterizations of N,N-bis(diphenylphosphino)ethylaniline derivatives and X-ray crystal structure of palladium (II), platinum (II) complexes DURAP, FEYYAZ; BİRİCİK, NERMİN; Guemguem, Bahattin; Özkar, Saim; Ang, Wee Han; Fei, Zhaofu; Scopelliti, Rosario (2008-01-20) N,N-Bis(diphenylphosphino)ethylaniline compounds, [Ph2P](2)N-C6H4-C2H5, with ethyl groups at the ortho- and para-positions have been synthesized. Oxidation of the aminophosphines with hydrogen peroxide, elemental sulfur and selenium gave the corresponding oxides, sulfides and selenides [Ph2P(E)](2)N-C6H4-C2H5 (E = O, S, Se). Complexes [MCl2{(Ph2P)(2)N-C6H4-(C2H5)}] (M = Pd, Pt) and [Cu{(Ph2P)(2)N-C6H4-C2H5)(2)]PF6 were obtained by the reaction of NN-bis(diphenylphosphino)ethylaniline with [MCl2(COD)] (M = P...
Evaluation of the Protective Effects of Quercetin, Rutin, Naringenin, Resveratrol and Trolox Against Idarubicin-Induced DNA Damage Çelik, Haydar; Arinç, Emel (University of Alberta Libraries, 2010-7-22) <jats:p>PURPOSE. Idarubicin is a synthetic anthracycline anticancer drug widely used in the treatment of some hematological malignancies. The studies in our laboratory have clearly demonstrated that idarubicin can undergo reductive bioactivation by NADPH-cytochrome P450 reductase to free radicals with resulting formation of DNA strand breaks, which can potentially contribute to its genotoxic effects [Çelik, H., Arinç, E., Bioreduction of idarubicin and formation of ROS responsible for DNA cleavage by NADPH-...
A theoretical study on camptothecin-AM1 treatment Türker, Burhan Lemi (2001-08-13) Camptothecin (irinotecan), a new cytotoxic agent having antitumor activity and especially used in the treatment of advanced colorectal cancers is considered for AM1-RBF type semiempirical quantum chemical treatment together with some analogs of camptothecin. The structural properties and energetics of these molecules have been compared. The structures arising from monoprotonation of camptothecin at various sites are analyzed from thermodynamic point of view.

Citation Formats

B. TURAL, S. B. Sopaci, N. Özkan, A. S. Demir, and M. Volkan, “Preparation and characterization of surface modified gamma-Fe2O3 (maghemite)-silica nanocomposites used for the purification of benzaldehyde lyase,” JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS, pp. 968–973, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31755.