Evaluation of the Protective Effects of Quercetin, Rutin, Naringenin, Resveratrol and Trolox Against Idarubicin-Induced DNA Damage

Çelik, Haydar
Arinç, Emel
<jats:p>PURPOSE. Idarubicin is a synthetic anthracycline anticancer drug widely used in the treatment of some hematological malignancies. The studies in our laboratory have clearly demonstrated that idarubicin can undergo reductive bioactivation by NADPH-cytochrome P450 reductase to free radicals with resulting formation of DNA strand breaks, which can potentially contribute to its genotoxic effects [Çelik, H., Arinç, E., Bioreduction of idarubicin and formation of ROS responsible for DNA cleavage by NADPH-cytochrome P450 reductase and its potential role in the antitumor effect. J Pharm Pharm Sci, 11(4):68-82, 2008]. In the current study, our aim was to investigate the possible protective effects of several phenolic antioxidants, quercetin, rutin, naringenin, resveratrol and trolox, against the DNA-damaging effect of idarubicin originating from its P450 reductase-catalyzed bioactivation. METHODS. DNA damage was measured by detecting single-strand breaks in plasmid pBR322 DNA using a cell-free agarose gel method. RESULTS. Our results indicated that, among the compounds tested, quercetin was the most potent antioxidant in preventing DNA damage. Quercetin significantly decreased the extent of DNA strand breaks in a dose-dependent manner; 100 μM of quercetin almost completely inhibited the DNA strand breakage. Unlike quercetin, its glycosidated conjugate rutin, failed to provide any significant protection against idarubicin-induced DNA strand breaks except at the highest concentration tested (2 mM). The protective effects of other antioxidants were significantly less than that of quercetin even at high concentrations. Quercetin was found to be also an effective protector against DNA damage induced by mitomycin C. CONCLUSION. We conclude that quercetin, one of the most abundant flavonoids in the human diet, is highly effective in reducing the DNA damage caused by the antitumor agents, idarubicin and mitomycin C, following bioactivation by P450 reductase.</jats:p>
Journal of Pharmacy & Pharmaceutical Sciences


Modeling dissolved oxygen dynamics and hypoxia
Peña, M. A.; Katsev, S.; Oguz, T.; Gilbert, D. (Copernicus GmbH, 2010-3-9)
<jats:p>Abstract. Hypoxia conditions are increasing throughout the world, influencing biogeochemical cycles of elements and marine life. Hypoxia results from complex interactions between physical and biogeochemical processes, which can not be understood by observations alone. Models are invaluable tools at studying system dynamics, generalizing discrete observations and predicting future states. They are also useful as management tools for evaluating site-specific responses to management scenarios. Here we ...
Modelling of Advective Transport and Biogeochemistry at a Shallow Hydrothermal Vent System
Bartlett, Stuart; Giovannelli, Donato; Yücel, Mustafa (2017-08-18)
Inthisworkwepresentthefirstinaseriesofmodelresultsaimedatelucidatingthecomplexcouplingsbetweenfluidflow,heattransport,advectivetransportofsolutes,andmicrobialmatgrowthatashallowhydrothermalvent.WeareprimarilyinterestedintheventsystemsofMilos,Greece,wherebiofilmcommunitiesarespatiallysegregatedintodistinctdomains,eachwithaverydifferentspeciescomposition[1].Thelocationsofthesedomainsvarystronglywiththesandbedtopography.Inparticular,therearesharpspeciescompositiondifferencesbetweenthewindwardsideand the leewar...
Modelling seasonal circulation and thermohaline structure of the Caspian Sea
Gunduz, M.; Özsoy, E. (Copernicus GmbH, 2014-6-10)
<jats:p>Abstract. The wind- and buoyancy-driven seasonal circulation of the Caspian Sea is investigated for a better understanding of its basin-wide and mesoscale dynamics, mixing and transport. The model successfully reproduces the following basic elements of the circulation: the southward-flowing current systems along the eastern and western coasts, the upwelling along the eastern coast, the cyclonic circulation in the Middle Caspian Sea (MCS), especially in winter, and the cyclonic and anticyclonic cells...
A theoretical study on camptothecin-AM1 treatment
Türker, Burhan Lemi (2001-08-13)
Camptothecin (irinotecan), a new cytotoxic agent having antitumor activity and especially used in the treatment of advanced colorectal cancers is considered for AM1-RBF type semiempirical quantum chemical treatment together with some analogs of camptothecin. The structural properties and energetics of these molecules have been compared. The structures arising from monoprotonation of camptothecin at various sites are analyzed from thermodynamic point of view.
Validation of the operational MSG-SEVIRI snow cover product over Austria
Surer, S.; Parajka, J.; Akyurek, Z. (Copernicus GmbH, 2014-2-24)
<jats:p>Abstract. The objective of this study is to evaluate the mapping accuracy of the MSG-SEVIRI operational snow cover product over Austria. The SEVIRI instrument is aboard the geostationary Meteosat Second Generation (MSG) satellite. The snow cover product provides 32 images per day, with a relatively low spatial resolution of 5 km over Austria. The mapping accuracy is examined at 178 stations with daily snow depth observations and compared with the daily MODIS-combined (Terra + Aqua) snow cover produc...
Citation Formats
H. Çelik and E. Arinç, “Evaluation of the Protective Effects of Quercetin, Rutin, Naringenin, Resveratrol and Trolox Against Idarubicin-Induced DNA Damage,” Journal of Pharmacy & Pharmaceutical Sciences, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/50961.