Enhanced salt tolerance of transgenic tobacco expressing a wheat salt tolerance gene

Date

2016-01-01

Author

KAVAS, MUSA
BALOĞLU, MEHMET CENGİZ
Yücel, Ayşe Meral
Öktem, Hüseyin Avni

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

227
views

0
downloads

Soil salinity is one of the most important limiting factors of agricultural productivity in the world. The Triticum aestivum salt tolerance-related gene (TaSTRG) possesses a functional response to salt and drought stress conditions. A variety of stress factors, such as salt, drought, abscisic acid, and cold, may induce the expression of TaSTRG in wheat. In this study, the TaSTRG gene was transferred to tobacco via Agrobacterium-mediated transformation. Overexpression of TaSTRG in transgenic tobacco plants indicated higher salt tolerance and mediated more vigorous growth than in wild-type plants. Under salt stress conditions, the transgenic tobacco plants had higher germination and survival rates and longer root length than the control plants. Under salt treatments (200-250 mM), TaSTRG-overexpressing tobacco plants accumulated a higher amount of proline and had significantly lower malondialdehyde content than wild-type plants. Furthermore, transgene inheritance followed Mendelian laws, indicating the stability of TaSTRG in transgenic tobacco plants. These results indicated that the wheat TaSTRG gene plays an important role in responding to salt stress.

Subject Keywords

Gateway cloning, Salt resistance, TaSTRG, Tobacco, Wheat

URI

https://hdl.handle.net/11511/31762

Journal

TURKISH JOURNAL OF BIOLOGY

DOI

https://doi.org/10.3906/biy-1506-36

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Transformation of tobacco with a NAC type transcription factor, tanac69-1 and characterization of transgenic plants via molecular and physiological techniques Eroğlu, Ayten; Yücel, Ayşe Meral; Öktem, Hüseyin Avni; Department of Biotechnology (2015) Environmental stresses such as drought and salinity greatly affect plant production leading to reduction in yield and quality. Stress tolerant plants can be rapidly produced by transferring stress related genes or transcription factors to crop plants. In this study, TaNAC69-1 gene, a NAC type transcription factor, was isolated from Triticum aestivum L. cv. Yüreğir-89 and cloned into a Gateway compatible overexpression vector, pEarleyGate 100. Transformation of Nicotiana tabacum L. cv. Samsun leaves was carr...
Physiological and biochemical screeing of different Turkish Lentil (Lens culinaris M.) cultivars under drought stress condition Gökçay, Derya; Yücel, Ayşe Meral; Baloğlu, Mehmet Cengiz; Department of Biotechnology (2012) Legumes being the most important crops worldwide are limited in terms of adaptability and productivity mainly by the abiotic stresses. In this study, the aim was to understand tolerance mechanisms of lentil cultivars under drought stress by physiological and biochemical analyses. This study was carried out with six Turkish Lentil cultivars (Seyran, Kafkas, Malazgirt, Çağıl, Çiftçi, Özbek) subjected to drought stresses (10% and 15% PEG) and their physiological and biochemical properties were examined to sele...
Particle bombardment transformation of some Turkish wheat cultivars with TaNAC69-1 and TtNAMB2 genes Battal, A.; BALOĞLU, MEHMET CENGİZ; Kavas, M.; Yücel, Mustafa; Öktem, Hüseyin Avni (2012-09-23) Wheat is one of the most important crop plants in the world.The production of wheat is seriously affected by biotic (pests,bacterial and fungal diseases) and abiotic (drought, salinity andfreezing) stresses. NAC family proteins (NAM/ATAF1-2/CUC2) areplant specific transcription factors and related with development,defense and abiotic stress responses.TaNAC69-1andTtNAMB2genes are the transcription factors from this protein family. Theaim of this study was to transform of some Turkish ...
Extension of flower longevity in transgenic plants via antisense blockage of ethylene bıosynthesis Deçani Yol, Betul; Öktem, Hüseyin Avni; Department of Biotechnology (2004) Ethylene (C2H4) is a very simple molecule, a gas, and has numerous effects on the growth, development and storage life of many fruits, vegetables and ornamental crops. In higher plants, ethylene is produced from L-methionine in essentially all tissues and ACC Synthase and ACC Oxidase are the two key enzymes in the biosynthesis of ethylene. The objective of the present study was to transform tobacco (Nicotiana tabacum L. cv. Samsun) plant with partial sequence of torenia acc oxidase gene in antisense and sen...
Identification and cloning of genes induced and / or repressed upon treatments of wheat plants (avocet s) with BTH, BABA and Trichoderma Harzianum Raifi KRL-AG2 Al-Asbahi, Adnan; Akkaya, Mahinur S.; Department of Biotechnology (2006) One of the major problems concerning the production of food crops is the controlling of plant diseases to maintain the high quality and yield. Wheat diseases are caused by parasitic bacteria, fungi and viruses that are a major hazard in wheat production. Therefore, understanding of any resistance mechanism is prerequisite for the successful utilization of wheat crop species in modern agriculture. The phenomenon of induced resistance by fungi, bacteria, microbial elicitors and chemicals has been investigated...

Citation Formats

M. KAVAS, M. C. BALOĞLU, A. M. Yücel, and H. A. Öktem, “Enhanced salt tolerance of transgenic tobacco expressing a wheat salt tolerance gene,” TURKISH JOURNAL OF BIOLOGY, pp. 727–735, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31762.