Particle bombardment transformation of some Turkish wheat cultivars with TaNAC69-1 and TtNAMB2 genes

Battal, A.
Kavas, M.
Yücel, Mustafa
Öktem, Hüseyin Avni
Wheat is one of the most important crop plants in the world.The production of wheat is seriously affected by biotic (pests,bacterial and fungal diseases) and abiotic (drought, salinity andfreezing) stresses. NAC family proteins (NAM/ATAF1-2/CUC2) areplant specific transcription factors and related with development,defense and abiotic stress responses.TaNAC69-1andTtNAMB2genes are the transcription factors from this protein family. Theaim of this study was to transform of some Turkish wheat culti-vars withTaNAC69-1andTtNAMB2using particle bombardment.After isolation ofTaNAC69-1fromTriticum aestivumandTtNAMB2fromTriticum turgidum, these genes were cloned intopAHC25,anoverexpression vector for monocots.pAHC25includesbargeneas a plant selectable marker gene. Plasmids includingTaNAC69-1andTtNAMB2were introduced into immature inflorescencesbased calli of Yure ̆gir-89 (T. aestivum) and Kızıltan-91 (T. turgidum)cultivars and mature embryo based calli of Mirzabey-2000 (T.turgidum) cultivar via particle bombardment. The bombarded calliwere regenerated previously optimized tissue culture and regener-ation conditions. After transformation of immature inflorescencebased callus, the regeneration rate was about 16.2% for Yure ̆gir-89 and Kızıltan-91. The rooting rate for these two cultivars was3.1%. The regeneration rate was 15.6% for Mirzabey-2000, witha 2.7% rooting rate. Putative transgenic plants were transferred tothe greenhouse for maturation. The studies on the molecular char-acterization of putative transgenic plants have been continued.


Genetic screening of Turkish wheat varieties for the durable resistance gene, Lr34
Boylu, Barış; Akkaya, Mahinur S.; Hakkı, Erdoğan Eşref; Department of Biotechnology (2011)
Wheat diseases such as rusts and powdery mildews are among the most important and ancient diseases that affect wheat cultivation worldwide. The pathogen race specific resistance genes cannot maintain long lasting resistance. On the other hand, the presence of genes confers the non-race specific resistance last much longer. The durable resistance phenotypes in wheat against various rust and powdery mildew diseases were reported as Lr34, Yr18, and Pm38 separately; nevertheless, they were known to locate very ...
Site directed mutations of one of the plant pathogen effector gene for functional analysis
Mustafa, Zemran; Akkaya, Mahinur S.; Department of Biochemistry (2015)
Wheat (Triticum aestivum L.) is one of the most important crops planted in the world and used as major food resource. Yet, there are major losses of productivity due to different biotic and abiotic destructive agents. Among the biotic factors especially the yellow rust is one of the most frequent. Yellow rust or also called “stripe rust”, due to stripe like appearances on the leaves, disease is caused by Puccinia striiformis f. sp. tritici, which is an obligate plant fungal pathogen. To cope with high yield...
Transformation of tobacco with a NAC type transcription factor, tanac69-1 and characterization of transgenic plants via molecular and physiological techniques
Eroğlu, Ayten; Yücel, Ayşe Meral; Öktem, Hüseyin Avni; Department of Biotechnology (2015)
Environmental stresses such as drought and salinity greatly affect plant production leading to reduction in yield and quality. Stress tolerant plants can be rapidly produced by transferring stress related genes or transcription factors to crop plants. In this study, TaNAC69-1 gene, a NAC type transcription factor, was isolated from Triticum aestivum L. cv. Yüreğir-89 and cloned into a Gateway compatible overexpression vector, pEarleyGate 100. Transformation of Nicotiana tabacum L. cv. Samsun leaves was carr...
Investigating the roles of micrornas in biotic stress responses and functional characterization of a novel ztl-type f-box protein via virus induced gene silencing
Dağdaş, Yasin Fatih; Akkaya, Mahinur S.; Department of Biotechnology (2009)
Barley and wheat are the two most important crop species in Turkey. Molecular studies for increasing crop yield of these species are very important for the economic benefits of Turkey. Powdery mildew and yellow rust are the two main pathogens, infecting barley and wheat, respectively in our country and causing a great amount of yield loss each year. Till now, classical genetics studies were performed in order to develop resistant barley and wheat cultivars, but these studies have not been succesful. Therefo...
TILLING for TtBH-1 gene and its hybridization to Turkish durum wheat cultivars Kızıltan-91 and Fuatbey-2000
Battal, Abdulhamit; Yücel, Ayşe Meral; Eyidoğan, Füsun; Department of Biotechnology (2016)
Wheat is one of the main crops to get daily calory requirement for people. Improving wheat yield is one of the most important aims in crop research today. Spike structure genes play a crucial role for yield increase. TtBH-A1 gene has a single nucleotide polymorphism at the conserved domain causing branched spikes producing more spikelet, grains and yield in “Miracle-Wheat”. In this study, TtBH-1 homoeologous (TtBH-A1 and TtBH-B1) gene were screened in tetraploid Kronos Targeting Induced Local Lesions In Gen...
Citation Formats
A. Battal, M. C. BALOĞLU, M. Kavas, M. Yücel, and H. A. Öktem, “Particle bombardment transformation of some Turkish wheat cultivars with TaNAC69-1 and TtNAMB2 genes,” NEW BIOTECHNOLOGY, pp. 0–0, 2012, Accessed: 00, 2020. [Online]. Available: