Particle bombardment transformation of some Turkish wheat cultivars with TaNAC69-1 and TtNAMB2 genes

2012-09-23
Battal, A.
BALOĞLU, MEHMET CENGİZ
Kavas, M.
Yücel, Mustafa
Öktem, Hüseyin Avni
Wheat is one of the most important crop plants in the world.The production of wheat is seriously affected by biotic (pests,bacterial and fungal diseases) and abiotic (drought, salinity andfreezing) stresses. NAC family proteins (NAM/ATAF1-2/CUC2) areplant specific transcription factors and related with development,defense and abiotic stress responses.TaNAC69-1andTtNAMB2genes are the transcription factors from this protein family. Theaim of this study was to transform of some Turkish wheat culti-vars withTaNAC69-1andTtNAMB2using particle bombardment.After isolation ofTaNAC69-1fromTriticum aestivumandTtNAMB2fromTriticum turgidum, these genes were cloned intopAHC25,anoverexpression vector for monocots.pAHC25includesbargeneas a plant selectable marker gene. Plasmids includingTaNAC69-1andTtNAMB2were introduced into immature inflorescencesbased calli of Yure ̆gir-89 (T. aestivum) and Kızıltan-91 (T. turgidum)cultivars and mature embryo based calli of Mirzabey-2000 (T.turgidum) cultivar via particle bombardment. The bombarded calliwere regenerated previously optimized tissue culture and regener-ation conditions. After transformation of immature inflorescencebased callus, the regeneration rate was about 16.2% for Yure ̆gir-89 and Kızıltan-91. The rooting rate for these two cultivars was3.1%. The regeneration rate was 15.6% for Mirzabey-2000, witha 2.7% rooting rate. Putative transgenic plants were transferred tothe greenhouse for maturation. The studies on the molecular char-acterization of putative transgenic plants have been continued.
Citation Formats
A. Battal, M. C. BALOĞLU, M. Kavas, M. Yücel, and H. A. Öktem, “Particle bombardment transformation of some Turkish wheat cultivars with TaNAC69-1 and TtNAMB2 genes,” pp. 0–0, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30703.