Polyester based nerve guidance conduit design

Yucel, Deniz
Hasırcı, Vasıf Nejat
Nerve conduits containing highly aligned architecture that mimics native tissues are essential for efficient regeneration of nerve injuries. In this study, a biodegradable nerve conduit was constructed by converting a porous micropatterned film (PHBV-P(L-D,L)LA-PLGA) into a tube wrapping aligned electrospun fibers (PHBV-PLGA). The polymers were chosen so that the protective tube would erode slower than the fibrous core to achieve complete healing before the tube eroded. The pattern dimensions and the porosity (58.95 (%) with a maximum pore size of 4-5 mu m) demonstrated that the micropatterned film would enable the migration, alignment and survival of native cells for proper regeneration. This film had sufficiently high mechanical properties (ultimate tensile strength: 3.13 MPa, Young's Modulus: 0.08 MPa) to serve as a nerve guide. Electrospun fibers, the inner part of the tubular construct, were well aligned with a fiber diameter of ca. 1.5 mu m. Fiber properties were especially influenced by polymer concentration. SEM showed that the fibers were aligned parallel to the groove axis of the micropatterned film within the tube as planned considering the nerve tissue architecture. This two component nerve conduit appears to have the right organization for testing in vitro and in vivo nerve tissue engineering studies.


Multiwalled CNT-pHEMA composite conduit for peripheral nerve repair.
Arslantunalı Şahin, Damla; Hasırcı, Vasıf Nejat (2014-03-01)
A nerve conduit is designed to improve peripheral nerve regeneration by providing guidance to the nerve cells. Conductivity of such guides is reported to enhance this process. In the current study, a nerve guide was constructed from poly(2-hydroxyethyl methacrylate) (pHEMA), which was loaded with multiwalled carbon nanotubes (mwCNT) to introduce conductivity. PHEMA hydrogels were designed to have a porous structure to facilitate the transportation of the compounds needed for cell nutrition and growth and al...
Differentiation of BMSCs into Nerve Precursor Cells on Fiber-Foam Constructs for Peripheral Nerve Tissue Engineering
Dursun Usal, Tuğba; YÜCEL, DENİZ; Hasırcı, Vasıf Nejat (2018-06-01)
Bone marrow stem cells (BMSCs) are frequently used in nerve tissue engineering studies due to ease of their isolation and high potential for differentiation into nerve cells. A bilayer fiber-foam construct containing nanofibrous elements to house and guide BMSCs was designed as a model to study the regeneration of damaged peripheral nerve tissue and eventually serve as a nerve guide. The construct consisted of a) a macroporous bottom layer to serve as the backing and support, and for nutrient transport, and...
Arslantunalı Şahin, Damla; Son, Çağdaş Devrim; Hasırcı, Vasıf Nejat; Department of Biotechnology (2022-2-1)
Any injury in peripheral nerves may result in a loss of neuronal communication along sensory and motor nerves, affecting patients’ daily activities. Today, there are various FDA approved commercial conduit materials; hollow tubes preventing them from used in gaps bigger than 10 mm, because they may lead axonal sprouts to form. The presented study includes pHEMA wrapping structure filled with GelMA-HaMA gel matrix as a nerve guidance channel. Following the structural analysis of the nerve guide, in vitro stu...
Wet spun PCL scaffolds for tissue engineering
Malikmammadov, Elbay; Hasırcı, Nesrin; Endoğan Tanır, Tuğba; Department of Micro and Nanotechnology (2017)
Scaffolds produced for tissue engineering applications are promising alternatives to be used in healing and regeneration of injured tissues and organs. In this study, fibrous poly(ε-caprolactone) (PCL) scaffolds were prepared by wet spinning technique and modified by addition of β-tricalcium phosphate (β-TCP) and by immobilizing gelatin onto fibers. Meanwhile, gelatin microspheres carrying Ceftriaxone sodium (CS), a model antibiotic, were added onto the scaffolds and antimicrobial activity of CS was investi...
Antibacterial Nanostructured Polyhydroxybutyrate Membranes for Guided Bone Regeneration
Karahaliloglu, Zeynep; Ercan, Batur; Taylor, Erik N.; Chung, Stanley; DENKBAŞ, EMİR BAKİ; Webster, Thomas J. (2015-12-01)
The principle of guided bone regeneration (GBR) in orthopedic, cranio-maxillofacial and dental tissue engineering applications is to create a secluded space for the treatment of large bone defects while excluding fibrous connective tissue formation at the defect area. In dental surgeries, a GBR membrane is placed near the dental implant in post-extraction sockets to grow new bone at the implant site, along with inhibiting infection due to the microbial nature of the mouth flora. Poly[(R)-3-hydroxybutyric ac...
Citation Formats
D. Yucel, G. KÖSE, and V. N. Hasırcı, “Polyester based nerve guidance conduit design,” BIOMATERIALS, pp. 1596–1603, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31890.