Speciation of selenium in vitamin tablets using spectrofluorometry following cloud point extraction

Guler, Nehir
Maden, Mervegul
Bakirdere, Sezgin
Ataman, Osman Yavuz
Volkan, Mürvet
Selenium is one of the trace and essential elements for good health but required only in a very narrow range. Hence, determination of selenium in trace level is important. In this work, cloud point extraction (CPE) with non-ionic surfactant Triton X-114 and the fluorometric ligand, 2,3-diaminonaphtalene (DAN) were used for extraction of trace amounts of organic and inorganic selenium species prior to their determination by spectrofluorometry. CPE parameters affecting complexation and phase separation were optimised. The limit of detection calculated by using nine replicate measurements of 0.020 mg/l. Se solution after complexing with DAN and 10-fold CPE preconcentration was 2.1 mu g/L. Accuracy of the method was checked using EnviroMat Waste Water, EU-L-2 as CRM and result was found to be in good agreement with the certified value. The suggested method can be used for selenium species of selenite, selenate, and total organic selenium at mu g/L level.


Spectrofluorimetric determination of organic and inorganic selenium in vitamin supplements after cloud point extraction
Maden, Mervegül; Volkan, Mürvet; Department of Chemistry (2010)
Selenium is a trace and essential element for good health but required only in very narrow range. Hence, determination of selenium in trace level in any matrix related with human health is important. A preconcentration method is performed to obtain a low detection limit for analyte. In this study, the methodology of cloud point extraction (CPE) was used as the preconcentration method for speciation of selenium in vitamin tablets. Non-ionic surfactant Triton X-114 and a fluorimetric ligand, 2,3- diaminonapht...
Studies on photochemical vapor generation of selenium
Çetmeli , Melis; Ertaş, Gülay; Department of Chemistry (2019)
Selenium is a significant trace element for human health. Selenium can be essential or toxic for human body depending on its concentration. Within the scope of this thesis, photochemical vapor generation tungsten coil atomization technique has been studied for the determination of selenium. Volatile selenium compounds were generated by exposure of UV light of the solution containing selenium and acetic acid. To increase sensitivity of photochemical vapor generation system, the technique was combined with tu...
Speciation studies using hplc-icp-ms and hplc-es-ms
Bakırdere, Sezgin; Ataman, Osman Yavuz; Department of Chemistry (2009)
Knowledge about selenium content of foods containing selenium species is very important in terms of both nutrition and toxicity. Bioavailability of selenium species for human body is different from each other. Hence, speciation of selenium is more important than total selenium determination. In the selenium speciation study, chicken breast samples, selenium supplement tablets and egg samples were analyzed for their selenium contents. In chicken breast study, chickens were randomly categorized into three gro...
Speciation of Selenium in Supplements by High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry
BAKIRDERE, Sezgin; Volkan, Mürvet; Ataman, Osman Yavuz (2015-06-13)
Anion and cation exchange high-performance liquid chromatography (HPLC) combined with inductively coupled plasma-mass spectrometry (ICP-MS) were used for speciation of selenium in supplements. All the parameters in the extraction, separation, and determination procedures were optimized. Recovery studies for the selenium species from the anion and cation exchange columns were performed and there were no analyte losses. Limits of detection for selenium(IV), selenium(VI), Se in selenomethionine, and Se in sele...
Application of Surface-Enhanced Raman Scattering (SERS) method for genetic analyses
Karabıçak, Seher; Volkan, Mürvet; Kocabıyık, Semra; Department of Chemistry (2011)
Raman spectroscopy offers much better spectral selectivity but its usage has been limited by its poor sensitivity. The discovery of surface-enhanced Raman scattering (SERS) effect, which results in increased sensitivities of up to 108-fold for some compounds, has eliminated this drawback. A new SERS active substrate was developed in this study. Silver nanoparticle-doped polyvinyl alcohol (PVA) coated SERS substrate prepared through chemical and electrochemical reduction of silver particles dispersed in the ...
Citation Formats
N. Guler, M. Maden, S. Bakirdere, O. Y. Ataman, and M. Volkan, “Speciation of selenium in vitamin tablets using spectrofluorometry following cloud point extraction,” FOOD CHEMISTRY, pp. 1793–1799, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31903.