Studies on photochemical vapor generation of selenium

Çetmeli , Melis
Selenium is a significant trace element for human health. Selenium can be essential or toxic for human body depending on its concentration. Within the scope of this thesis, photochemical vapor generation tungsten coil atomization technique has been studied for the determination of selenium. Volatile selenium compounds were generated by exposure of UV light of the solution containing selenium and acetic acid. To increase sensitivity of photochemical vapor generation system, the technique was combined with tungsten-coil atomization. Generated volatile selenium species were trapped and atomized on the rhodium coated tungsten coil. Among Ru, Rh, Ir, Co and Pd coated tungsten coils, selenium signal was sharper, more reproducible and more sensitive with rhodium coated tungsten coil. The experimental parameters were optimized. LOD and LOQ of the technique were determined as 3.3 and 11.1 μg/L respectively. Technique was applied to selenium dietary supplements containing sodium selenite. The concentration of selenium in tablets was determined by using photochemical vapor generation tungsten-coil atomization method. The result is consistent with the certificate value given by the company. Interference from PVG-amenable elements, transition metals and earth base elementswas investigated. The most severe interference effect on the signal of Se (IV) was observed from copper.


Spectrofluorimetric determination of organic and inorganic selenium in vitamin supplements after cloud point extraction
Maden, Mervegül; Volkan, Mürvet; Department of Chemistry (2010)
Selenium is a trace and essential element for good health but required only in very narrow range. Hence, determination of selenium in trace level in any matrix related with human health is important. A preconcentration method is performed to obtain a low detection limit for analyte. In this study, the methodology of cloud point extraction (CPE) was used as the preconcentration method for speciation of selenium in vitamin tablets. Non-ionic surfactant Triton X-114 and a fluorimetric ligand, 2,3- diaminonapht...
A biomechanical and spectroscopic study of bone from rats with selenium deficiency and toxicity
Turan, B; Bayari, S; Balcik, C; Severcan, Feride; Akkas, N (2000-06-01)
Selenium, being an essential mineral in the mammalian diet, is important in providing protection against oxidative damage. Numerous in vitro studies of selenium compounds reveal a very high correlation between catalytic activity of selenium compounds and toxicity. The present study was designed to investigate the effects of dietary selenium on the biomechanical properties of bone. New born rats of both sexes were fed with either a control, or a selenium- and vitamin E-deficient, or a selenium-excess and vit...
Speciation of selenium in vitamin tablets using spectrofluorometry following cloud point extraction
Guler, Nehir; Maden, Mervegul; Bakirdere, Sezgin; Ataman, Osman Yavuz; Volkan, Mürvet (2011-12-15)
Selenium is one of the trace and essential elements for good health but required only in a very narrow range. Hence, determination of selenium in trace level is important. In this work, cloud point extraction (CPE) with non-ionic surfactant Triton X-114 and the fluorometric ligand, 2,3-diaminonaphtalene (DAN) were used for extraction of trace amounts of organic and inorganic selenium species prior to their determination by spectrofluorometry. CPE parameters affecting complexation and phase separation were o...
Speciation studies using hplc-icp-ms and hplc-es-ms
Bakırdere, Sezgin; Ataman, Osman Yavuz; Department of Chemistry (2009)
Knowledge about selenium content of foods containing selenium species is very important in terms of both nutrition and toxicity. Bioavailability of selenium species for human body is different from each other. Hence, speciation of selenium is more important than total selenium determination. In the selenium speciation study, chicken breast samples, selenium supplement tablets and egg samples were analyzed for their selenium contents. In chicken breast study, chickens were randomly categorized into three gro...
Characterization of aldrin epoxidation in flathead mullet (Mugil cephalus) liver microsomes: Involvement of CYP3A
BOZCAARMUTLU, AZRA; Turna, S.; Erden, C. Sapmaz; Yenisoy-Karakas, S.; Arinc, E. (2011-08-28)
Toxic organochlorine pesticides, aldrin and its metabolite dieldrin, have been reported to contaminate the aquatic environment highly. In the present study, epoxidation reaction of aldrin to dieldrin was studied in mullet liver microsomes and contribution of cytochrome P450 isozyme(s) was determined by using specific cytochrome P450 inhibitors and substrate. Flathead mullet samples (Mugil cephalus) were caught from the West Black Sea Region of Turkey. Fish liver microsomes were prepared by differential cent...
Citation Formats
M. Çetmeli, “Studies on photochemical vapor generation of selenium,” Thesis (M.S.) -- Graduate School of Natural and Applied Sciences. Chemistry., Middle East Technical University, 2019.