Development of a UV crosslinked biodegradable hydrogel containing adipose derived stem cells to promote vascularization for skin wounds and tissue engineering

2017-06-01
Eke, Gozde
Mangir, Naside
Hasırcı, Nesrin
MacNeil, Sheila
Hasırcı, Vasıf Nejat
Show full item record
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
180
views
0
downloads
The aim of this study was to design a dermal substitute containing adipose derived stem cells (ADSC) that can be used to improve the regeneration of skin on difficult wound beds by stimulating rapid neovascularization. This was achieved by first synthesizing methacrylated gelatin (GeIMA) and methacrylated hyaluronic acid (HAMA) precursors which could be stored at -80 degrees C after lyophilisation. Polymer precursors were then dissolved in media (in 15:1 ratio), ADSCs added together with the photoinitiator and crosslinked with 40 s of W. Hydrogels degraded by 50% over 3 weeks in an in vitro environment. ADSC loaded hydrogels could be easily handled with forceps (compressive modulus was 6 kPa). Transparency of the gel would allow a full field-of-view of a wound site. The hydrogels provided a suitable microenvironment for ADSC proliferation as shown by the filopodia observed in confocal micrographs. In vivo studies demonstrated that stem cell loaded hydrogels increased vascularization by up to 3 fold compared to their cell free counterparts. In conclusion, GelMA/HAMA hydrogels loaded with ADSC showed the desired proliferative and angiogenic properties essential to promote angiogenesis for wound healing and improving survival of tissue engineered skin.
Dermal substitute, Bicomponent hydrogel, Adipose derived stem cells, Photocrosslinking, Angiogenesis
https://hdl.handle.net/11511/32140
Graduate School of Natural and Applied Sciences, Article

Suggestions

Development of a calcium phosphate-gelatin composite as a bone substitute and its use in drug release
Yaylaoglu, MB; Korkusuz, P; Ors, U; Korkusuz, F; Hasırcı, Vasıf Nejat (1999-04-01)
This study was carried out to develop a calcium phosphate-gelatin composite implant that would mimic the structure and function of bone for use in filling voids or gaps and to release bioactive compounds like drugs, growth hormones into the implant site to assist healing. XDS analysis of the synthesized calcium phosphate revealed a calcium to phosphorus molar ratio of ca. 2.30, implying a less erodible material than hydroxyapatite (1.67). Release of the antibiotic gentamicin from the implant was with a burs...
Development of a novel functionally graded membrane containing boron-modified bioactive glass nanoparticles for guided bone regeneration
Rad, Reza Moonesi; Atila, Deniz; Evis, Zafer; Keskin, Dilek; Tezcaner, Ayşen (2019-08-01)
Barrier membranes are used in periodontal tissue engineering for successful neo-bone tissue formation and prevention of bacterial colonization. We aimed to prepare and characterize novel 7% boron-modified bioactive glass (7B-BG) containing bilayered membrane for this end. We hypothesized that presence of 7B-BG could promote structural and biological properties of guided bone regeneration (GBR) membrane. Cellulose acetate (CA) layer was prepared by solvent casting, and functionally graded layer of CA/gelatin...
Characterization and identification of human mesenchymal stem cells at molecular level
Aksoy, Ceren; Severcan, Feride; Çetinkaya, Duygu Uçkan; Department of Biotechnology (2012)
Bone marrow mesenchymal stem cells (BM-MSCs) are pluripotent cells that can differentiate into a variety of non-hematopoietic tissues. They also maintain healthy heamatopoiesis by providing supportive cellular microenvironment into BM. In this thesis, MSCs were characterized in terms of their morphological, immunophenotypical and differentiation properties. Then, they were examined by attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy together with hierarchical clustering, and FT...
EVALUATION OF THE CHITOSAN-COATING EFFECTIVENESS ON A DENTAL TITANIUM ALLOY IN TERMS OF MICROBIAL AND FIBROBLASTIC ATTACHMENT AND THE EFFECT OF AGING
Kalyoncuoglu, Ulku Tugba; Yilmaz, Bengi; Gungor, Serap; Evis, Zafer; UYAR, PEMBEGÜL; AKCA, GÜLÇİN; Kansu, Gulay (2015-01-01)
The aim of this study was to obtain a biocompatible and antimicrobial implant surface by coating Ti6Al4V with chitosan which can be used to create a smooth transmucosal region for a faster and better wound healing and an increased bioactivity. Ti6Al4V plates were first abraded and ultrasonically cleaned and then coated with chitosan. In order to simulate the conditions of an oral environment, a group of coated plates were treated in a thermocycle apparatus. The coatings were evaluated with SEM, EDS, XRD and...
DEVELOPMENT OF 3D PRINTED PECTIN AND GELATIN BASED BIOACTIVE SKIN GRAFTS
Bostancı, Nazlı Seray; Tezcaner, Ayşen; Hasırcı, Nesrin; Department of Biotechnology (2021-8-10)
The aim of this study was to develop an easily applicable and bioactive skin graft composed of methacrylated pectin (PeMA) and methacrylated gelatin (GelMA), containing curcumin and/or Vitamin-C as antimicrobial and healing accelerating agents, respectively. Grafts were prepared in slab, 3D printed and 3D bioprinted forms and different properties were evaluated. Preparation parameters were optimized using slabs having different compositions (PeMA:GelMA as P:G 1:1, 1:2 and 1:3, v/v). P1:G1 solution did not h...
Citation Formats
G. Eke, N. Mangir, N. Hasırcı, S. MacNeil, and V. N. Hasırcı, “Development of a UV crosslinked biodegradable hydrogel containing adipose derived stem cells to promote vascularization for skin wounds and tissue engineering,” BIOMATERIALS, pp. 188–198, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32140.
METU IIS - Integrated Information Service open@metu.edu.tr