Detection of imatinib resistance in K562 leukemia cells by 3D-electrode contactless dielectrophoresis

Date

2013-12-01

Author

Demircan, Y.
Koyuncuoglu, A.
Erdem, Murat
Ozgur, E.
Gündüz, Ufuk
Külah, Haluk

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This paper presents the trapping of imatinib resistant K562 (human chronic myelogenic leukemia (CML), K562/IMA) cells with 3D-electrode contactless dielectrophoresis (DEP). 3D electrodes are isolated from the solution by means of uniform thin parylene layer (~0.3μm), to eliminate the Joule heating, electrolysis, and cell damaging. 3D electrodes (extruded along the microchannel in z-axis) provide uniform distribution of DEP force along the channel height, improving the separation efficiency, significantly. It is verified that the system is capable of trapping K562/IMA cells at a concentration of 6.25×10 5 /ml and 10 μl/min flow rate by applying 9 V pp sinusoidal signal at 48.64 MHz. No trapping occurs for K562 sensitive cells at the same experimental conditions.

Subject Keywords

K562, 3D-electrode, Contacless dielectrophoresis (DEP), Label-free analysis, Multidrug resistance (MDR)

URI

https://hdl.handle.net/11511/32224

DOI

https://doi.org/10.1109/transducers.2013.6627211

Collections

Graduate School of Natural and Applied Sciences, Conference / Seminar

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Citation Formats

Y. Demircan, A. Koyuncuoglu, M. Erdem, E. Ozgur, U. Gündüz, and H. Külah, “Detection of imatinib resistance in K562 leukemia cells by 3D-electrode contactless dielectrophoresis,” 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32224.