Immobilization of heparin on chitosan-grafted polyurethane films to enhance anti-adhesive and antibacterial properties

Date

2016-01-01

Author

Kara, Filiz
AKSOY, EDA AYŞE
ÇALAMAK, SEMİH
Hasırcı, Nesrin
AKSOY, SERPİL

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

151
views

0
downloads

Infections caused by bacteria adhering to implant surfaces are one of the main reasons for the failure of the implants. In this study, polyurethane (PU), which is the most commonly used polymer in the production of medical devices, was synthesized and surfaces of polyurethane films were modified by chitosan (CH) grafting and heparin (Hep) immobilization in order to enhance anti-adhesiveness and antibacterial properties. Functional groups present on the surface, topographical shapes, and free energies of the polyurethane films were determined. Pristine polyurethane, chitosan-grafted polyurethane (PU-CH), and heparin immobilized polyurethane (PU-CH-Hep) films demonstrated high anti-adhesive efficacy against bacteria in the given order, where PU-CH-Hep was the most effective one. When PU-CH-Hep samples were incubated with different bacteria, complete death was observed for Pseudomonas aeruginosa (Gram negative), Staphylococcus aureus (Gram positive), and Staphylococcus epidermidis (Gram positive). Some living Escherichia coli (Gram negative) were observed after 24h of incubation. Pristine and modified polyurethane samples demonstrated no adverse effect on proliferation of L929 fibroblast cells and were found to be biocompatible according to MTT cytotoxicity tests.

Subject Keywords

Polyurethane, Chitosan, Heparin, Anti-adhesive, Antibacterial, Biocompatibility

URI

https://hdl.handle.net/11511/32316

Journal

JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS

DOI

https://doi.org/10.1177/0883911515598794

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Additive manufacturing of biodegradable magnesium implants and scaffolds: Review of the recent advances and research trends Sezer, Nurettin; Evis, Zafer; Koc, Muammer (2021-03-01) Synthetic grafting needs improvements to eliminate secondary surgeries for the removal of implants after healing of the defected tissues. Tissue scaffolds are engineered to serve as temporary templates, which support the affected tissue and gradually degrade through the healing period. Beside mechanical function to withstand the anatomic loading conditions, scaffolds should also provide a decent biological function for the diffusion of nutrients and oxygen to the cells, and excretion of the wastes from the ...
Investigation of surface structure and biocompatibility of chitosan-coated zirconia and alumina dental abutments Kalyoncuoglu, Ulku Tugba; Yilmaz, Bengi; Koc, Serap Gungor; Evis, Zafer; ARPACI, PEMBEGÜL UYAR; Kansu, Gulay (Wiley, 2018-12-01) Background: For long-term success of dental implants, it is essential to maintain the health of the surrounding soft tissue barrier, which protects the bone-implant interface from the microorganisms. Although implants based on titanium and its alloys still dominate the dental implant market, alumina (Al2O3) and zirconia (ZrO2) implant systems are widely used in the area. However, they provide smooth and bioinert surfaces in the transmucosal region, which poorly integrate with the surrounding tissues.
Antimicrobial performance of mesoporous titania thin films: role of pore size, hydrophobicity, and antibiotic release Atefyekta, Saba; Ercan, Batur; Karlsson, Johan; Taylor, Erik; Chung, Stanley; Webster, Thomas J.; Andersson, Martin (2016-01-01) Implant-associated infections are undesirable complications that might arise after implant surgery. If the infection is not prevented, it can lead to tremendous cost, trauma, and even life threatening conditions for the patient. Development of an implant coating loaded with antimicrobial substances would be an effective way to improve the success rate of implants. In this study, the in vitro efficacy of mesoporous titania thin films used as a novel antimicrobial release coating was evaluated. Mesoporous tit...
Effects of different sterilization techniques and varying anodized TiO2 nanotube dimensions on bacteria growth Kummer, Kim M.; Taylor, Erik N.; Durmas, Naside G.; Tarquinio, Keiko M.; Ercan, Batur; Webster, Thomas J. (Wiley, 2013-07-01) Infection of titanium (Ti)-based orthopedic implants is a growing problem due to the ability of bacteria to develop a resistance to today's antibiotics. As an attempt to develop a new strategy to combat bacteria functions, Ti was anodized in the present study to possess different diameters of nanotubes. It is reported here for the first time that Ti anodized to possess 20 nm tubes then followed by heat treatment to remove fluorine deposited from the HF anodization electrolyte solution significantly reduced ...
Investigation of thin semiconductor coatings and their antimicrobial properties Erkan, Arcan; Karakaş, Gürkan; Department of Chemical Engineering (2005) Regular disinfection of surfaces is required in order to reduce the number of microorganisms, unable to transmit infections and maintaining the surfaces sterilized. For this purpose, antimicrobial thin film coatings on the various surfaces such as glass and ceramic surfaces, capable of killing harmful microorganisms are being investigated. Generally a semiconducting material which can be activated by UV light tends to exhibit a strong antimicrobial activity. With holes (h+) and hydroxyl radicals (OH*) gener...

Citation Formats

F. Kara, E. A. AKSOY, S. ÇALAMAK, N. Hasırcı, and S. AKSOY, “Immobilization of heparin on chitosan-grafted polyurethane films to enhance anti-adhesive and antibacterial properties,” JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, pp. 72–90, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32316.