In this study liposomes composed of only dicetylphosphate (DCP), or of a 7:2: 1 (mole ratio) mixture of phosphatidylcholine (PC), dicetylphosphate (DCP) and cholesterol (Chol.( were prepared. Resultant liposomes were coated with varying amounts of poly(1,4‐pyridinium diethylene salt(, P1,4 VN. The polymer was shown to be different from poly(4‐vinylpyridine(, P4VN, which is also obtained from the same monomer. The increase in the coating extent (or thickness( was shown by IR and UV through the increase in the peaks due to polymer. The strength of binding was indirectly shown by being able to obtain scanning electron micrographs for the coated liposomes while it was not possible for the uncoated ones. It was shown that the lipid membrane under the polymeric coat can be removed. The resultant structure is highly permeable in comparison to the impermeable, lipid‐containing form. The activity of the enzyme glucose oxidase was demonstrated using the enzyme encapsulated in the polymeric net.


Production and biochemical characterization of polyphenol oxidase from thermomyces lanuginosus
Astarcı, Erhan; Bakır, Ufuk; Ögel, Zümrüt B.; Department of Biotechnology (2003)
Polyphenol oxidases are enzymes that catalyze the oxidation of certain phenolic substrates to quinones in the presence of molecular oxygen. Polyphenol oxidases are widely used in several applications. In food industry, they are used for enhancement of flavor in coffee, tea and cocoa production, and determination of food quality. In medicine, they have several uses in treatments of Parkinson̕s disease, phenlyketonurea and leukemia. In wastewater treatment, they are used for the removal of phenolic pollutants...
Enzyme catalyzed reverse enantiomeric separation of methyl (+/-)-3-cyclohexene-1-carboxylate
Tanyeli, Cihangir (Elsevier BV, 2004-07-01)
We describe the differences of hydrolase-type enzymes pig liver esterase (PLE), horse liver esterase (HLE), and porcine pancreatic lipase (PPL) on the hydrolysis of methyl (+/-)-3-cyclohexene-1-carboxylate to afford both enantiomers with 89% to > 99% ee. The resultant enantiomerically pure (S)-(-)-3-cyclohexene-1-carboxylic acid was transformed into (1S,5S)-(-)-5-(hydroxymethyl)-2-cyclohexen-1-ol via iodolactonization, subsequent elimination of iodine with 1,8-diazibicyclo[5.4.0]undec-7-ene (DBU) and reduct...
GURSEL, I; Hasırcı, Vasıf Nejat (1992-01-01)
In this study, matrix entrapment of the enzyme glucose oxidase was achieved through gamma-irradiation of monomers N-vinyl pyrrolidone, 2-hydroxyethyl methacrylate and their mixture. To test the effect of radiation on entrapment efficiency, retention of activities and properties of the system, duration and temperature were varied. The reusability of the resultant products was tested. It was generally found that inclusion of the hydrophilic monomer N-vinyl pyrrolidone into the matrix increased the water conte...
Enzyme enhanced ultrafiltration for the resolution of racemic mandelic acid
Kavurt, Ülkü Bade; Çalık, Pınar; Department of Chemical Engineering (2011)
In this study, resolution of racemic mandelic acid by enyzme enhanced ultrafiltration (EEUF) was studied. In order to develop a methodology, bovine serum albumin (BSA) was used as a model protein for polymer enhanced ultrafiltration (PEUF) experiments and the enzyme S-mandelate dehydrogenase was used for EEUF experiments. To be used for enzyme enhanced ultrafiltration experiments, the gene which is responsible from the production of S-mandelate dehydrogenase was isolated from Pseudomonas putida, expressed i...
Effect of Cr and Mo doping on the electrochemical properties of freeze-dried LiCoO2
Buyukburc, Atil; Aydınol, Mehmet Kadri (2014-10-01)
In this study, LiCoO2 is doped with 2 mol.% Cr and Mo in order to determine the effect of these elements on the electrochemical performance. Precursors are synthesized by means of freeze drying. Calcination is applied at 750 degrees C for 24 h. Doping content and calcination temperature as well as duration are very important for producing impurity-free layered LiCoO2. If the doping content is larger than 2 mol.% or when the calcination temperature is decreased to 550 degrees C, impurity phases are seen. As ...
Citation Formats
M. OZDEN and V. N. Hasırcı, “ENZYME IMMOBILIZATION IN POLYMER COATED LIPOSOMES,” BRITISH POLYMER JOURNAL, pp. 229–234, 1990, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32417.