ENZYME IMMOBILIZATION IN POLYMER COATED LIPOSOMES

Date

1990-01-01

Author

OZDEN, MY
Hasırcı, Vasıf Nejat

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In this study liposomes composed of only dicetylphosphate (DCP), or of a 7:2: 1 (mole ratio) mixture of phosphatidylcholine (PC), dicetylphosphate (DCP) and cholesterol (Chol.( were prepared. Resultant liposomes were coated with varying amounts of poly(1,4‐pyridinium diethylene salt(, P1,4 VN. The polymer was shown to be different from poly(4‐vinylpyridine(, P4VN, which is also obtained from the same monomer. The increase in the coating extent (or thickness( was shown by IR and UV through the increase in the peaks due to polymer. The strength of binding was indirectly shown by being able to obtain scanning electron micrographs for the coated liposomes while it was not possible for the uncoated ones. It was shown that the lipid membrane under the polymeric coat can be removed. The resultant structure is highly permeable in comparison to the impermeable, lipid‐containing form. The activity of the enzyme glucose oxidase was demonstrated using the enzyme encapsulated in the polymeric net.

Subject Keywords

poly(4‐vinylpyridine), Liposome, Glucose oxidase, Enzyme encapsulation

URI

https://hdl.handle.net/11511/32417

Journal

BRITISH POLYMER JOURNAL

DOI

https://doi.org/10.1002/pi.4980230309

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

M. OZDEN and V. N. Hasırcı, “ENZYME IMMOBILIZATION IN POLYMER COATED LIPOSOMES,” BRITISH POLYMER JOURNAL, pp. 229–234, 1990, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32417.