Induction of reactive oxygen species in marine phytoplankton under crude oil exposure

Date

2015-12-01

Author

Özhan, Koray
SMITH, Aaron P.
BARGU, Sibel

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

145
views

0
downloads

Exposure of phytoplankton to the water-accommodated fraction of crude oil can elicit a number of stress responses, but the mechanisms that drive these responses are unclear. South Louisiana crude oil was selected to investigate its effects on population growth, chlorophyll a (Chl a) content, antioxidative defense, and lipid peroxidation, for the marine diatom, Ditylum brightwellii, and the dinoflagellate, Heterocapsa triquetra, in laboratory-based microcosm experiments. The transcript levels of several possible stress-responsive genes in D. brightwellii were also measured. The microalgae were exposed to crude oil for up to 96 h, and Chl a content, superoxide dismutase (SOD), the glutathione pool (GSH and GSSG), and lipid peroxidation content were analyzed. The cell growth of both phytoplankton species was inhibited with increasing crude oil concentrations. Crude oil exposure did not affect Chl a content significantly in cells. SOD activities showed similar responses in both species, being enhanced at 4- and 8-mg/L crude oil exposure. Only H. triquetra demonstrated enhanced activity in GSSG pool and lipid peroxidation at 8-mg/L crude oil exposure, suggesting that phytoplankton species have distinct physiological responses and tolerance levels to crude oil exposure. This study indicated the activation of reactive oxygen species (ROS) in phytoplankton under crude oil exposure; however, the progressive damage in cells is still unknown. Thus, ROS-related damage in nucleic acid, lipids, proteins, and DNA, due to crude oil exposure could be a worthwhile subject of study to better understand crude oil toxicity at the base of the food web.

Subject Keywords

Phytoplankton, Crude oil, The deepwater horizon oil spill, Reactive oxygen species

URI

https://hdl.handle.net/11511/32458

Journal

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH

DOI

https://doi.org/10.1007/s11356-015-5037-y

Collections

Graduate School of Marine Sciences, Article

Suggestions

OpenMETU
Core

Effect of brine composition on oil recovery by waterflooding Bagci, S; Kök, Mustafa Verşan; Türksoy, Ulaş (2001-01-01) In this study, the effect of brine composition on oil recovery by waterflooding was experimentally studied. Different compositions of brine were injected in to packed one-dimensional unconsolidated limestone with Garzan crude oil (26 degrees API) and distilled water. Ten different brine compositions, three brine mixtures are prepared and distilled water was used for injection throughout the study. The highest recovery 18.8% higher than distilled water injection) considering all the experiments was obtained ...
Can Crude Oil Toxicity on Phytoplankton Be Predicted Based on Toxicity Data on Benzo(a)Pyrene and Naphthalene? Özhan, Koray (2014-02-01) Polycyclic aromatic hydrocarbons (PAHs), which are major components of crude oil, are responsible in large part for the toxicity of crude oil to phytoplankton. This study addressed the following question. Can reliable predictions of the aquatic toxicity of crude oil, a multi-component mixture, be described from toxicity data on individual PAH compounds? Naphthalene, the most abundant PAH compound, and benzo(a)pyrene, a highly toxic PAH compound, were selected as model compounds to quantify toxicity of crude...
Effect of different clay concentrations on crude oil combustion kinetics by thermogravimetry Kök, Mustafa Verşan (2010-03-01) The objective of this research was to investigate the effect of different clay composition and concentrations on the thermal behaviour and kinetics of heavy crude oil in limestone matrix by thermogravimetry (TG/DTG). In TG/DTG experiments, three distinct reaction regions were identified in all of the crude oil + limestone mixture known as low temperature oxidation (LTO), fuel deposition (FD) and high temperature oxidation (HTO) respectively. Addition of clay to porous matrix significantly affected the react...
Effects of CO₂ saturation on the recovery of the heavy oil using steam injection EOR technique Farid, Guluzade; Sınayuç, Çağlar; Department of Petroleum and Natural Gas Engineering (2014) Enhanced Oil Recovery processes include all methods that use external sources of energy and/or materials to recover oil that cannot be produced economically by conventional means. EOR processes can be classified as: thermal methods (in-situ combustion, cyclic steam injection, steam flooding), chemical methods (alkaline, polymer, foam, or surfactant/polymer injection), and miscible methods (CO2, nitrogen, hydrocarbon, or flue gas injection). Steam flooding involves continuous injection of steam to displace o...
AN OIL SPILL DISTRIBUTION STUDY IN AN INDUSTRIAL COASTAL ZONE KABDASLİ, M Sedat; KAÇMAZ, Erkan; BAŞ, BİLGE; Oğuz, Elif; BAĞCI, TAYLAN (2010-01-01) Numerical modeling studies are carried out to determine the wind and current effects on oil spill distribution. The study is performed by using a simulation software named OILMAP for estimating oil spill distribution together with AQUASEA for obtaining the current pattern in the marine environment. For this purpose, various simulation scenarios are created for the Gulf of Izmit, which is an important industrial coastal zone in the north-west of Turkey. In simulation scenarios, constant and variable wind dir...

Citation Formats

K. Özhan, A. P. SMITH, and S. BARGU, “Induction of reactive oxygen species in marine phytoplankton under crude oil exposure,” ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, pp. 18874–18884, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32458.