Direct evidence of advantage of using nanosized zeolite Beta for ISFET-based biosensor construction

Soy, Esin
Galioglu, Sezin
Soldatkin, Oleksandr O.
Dzyadevych, Sergei V.
Warzywoda, Juliusz
Sacco, Albert
Akata Kurç, Burcu
Analytical characteristics of urease- and butyrylcholinesterase (BuChE)-based ion sensitive field-effect transistor (ISFET) biosensors were investigated by the incorporation of zeolite Beta nanoparticles with varying Si/Al ratios. The results obtained by the zeolite-modified ISFET transducers suggested that the Si/Al ratio strongly influenced the biosensor performances due to the electrostatic interactions among enzyme, substrate, and zeolite surface as well as the nature of the enzymatic reaction. Using relatively small nanoparticles (62.7 +/- 10, 76.2 +/- 10, and 77.1 +/- 10 nm) rather than larger particles, that are widely used in the literature, allow us to produce more homogenous products which will give more control over the quantity of materials used on the electrode surface and ability to change solely Si/Al ratio without changing other parameters such as particle size, pore volume, and surface area. This should enable the investigation of the individual effect of changing acidic and electronic nature of this material on the biosensor characteristics. According to our results, high biosensor sensitivity is evident on nanosize and submicron size particles, with the former resulting in higher performance. The sensitivity of biosensors modified by zeolite particles is higher than that to the protein for both types of biosensors. Most significantly, our results show that the performance of constructed ISFET-type biosensors strongly depends on Si/Al ratio of employed zeolite Beta nanoparticles as well as the type of enzymatic reaction employed. All fabricated biosensors demonstrated high signal reproducibility and stability for both BuChE and urease.


Investigation of characteristics of urea and butyrylcholine chloride biosensors based on ion-selective field-effect transistors modified by the incorporation of heat-treated zeolite Beta crystals
Soy, Esin; Arkhypova, Valentyna; Soldatkin, Oleksandr; Shelyakina, Margarita; Dzyadevych, Sergei; Warzywoda, Juliusz; Sacco, Albert; Akata Kurç, Burcu (2012-10-01)
Urea and butyrylcholine chloride (BuChCl) biosensors were prepared by adsorption of urease and butyrylcholinesterase (BuChE) on heat-treated zeolite Beta crystals, which were incorporated into membranes deposited on ion-selective field-effect transistor (ISFET) surfaces. The responses, stabilities, and use for inhibition analysis of these biosensors were investigated. Different heat treatment procedures changed the amount of Bronsted acid sites without affecting the size, morphology, overall Si/Al ratio, ex...
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Akin, Deniz; YAKAR, ARZU; Gündüz, Ufuk (2013-04-28)
The aim of this study is to synthesize magnetic Fe3O4-chitosan nano and microparticles (Fe3O4-CPs) by suspension cross-linking and ionic gelation methods and investigate the effect of ultrasonication on the size, morphology and magnetic properties. The synthesized particles were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and vibrating sample magnetometry (VSM). The results showed that the ultrasonication decreased the mean particle diameter and enhanced magnetic ...
Applications of ions produced by low intensity repetitive laser pulses for implantation into semiconductor materials
Wolowski, J.; Badziak, J.; Czarnecka, A.; Parys, P.; Pisarek, M.; Rosinski, M.; Turan, Raşit; Yerci, Selçuk (2008-01-01)
This work reports experiment concerning specific applications of implantation of laser-produced ions for production of semiconductor nanocrystals. The investigation was carried out in the IPPLM within the EC STREP 'SEMINANO' project. A repetitive pulse laser system of parameters: energy up to 0.8 J in a 3.5 ns-pulse, wavelength of 1.06 mu m, repetition rate of up to 10 Hz, has been employed in these investigations. The characterisation of laser-produced ions was performed with the use of 'time-of-flight' io...
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This thesis deals with design and synthesis of chiral polyurea type polymers. These polymers were analyzed with molecular mechanics that they could take a helical form. The polymers are polyurea type polymers and ureas are synthesized by treating isocyanates with amines. Starting from tartaric acid, ((4S,5S)-2,2-dimethyl-1,3- dioxolane-4,5diyl)dimethaneamine was synthesized. The other diamine was naturally occuring L-lysine. These diamine functionalized compounds were treated with pphenylene diisocyanate sy...
Biosensors Based on Nano-Gold/Zeolite-Modified Ion Selective Field-Effect Transistors for Creatinine Detection
Kasap, Berna Ozansoy; Marchenko, Svitlana V.; Soldatkin, Oleksandr O.; Dzyadevych, Sergei V.; Akata Kurç, Burcu (2017-03-02)
The combination of advantages of using zeolites and gold nanoparticles were aimed to be used for the first time to improve the characteristic properties of ion selective field-effect transistor (ISFET)-based creatinine biosensors. The biosensors with covalently cross-linked creatinine deiminase using glutaraldehyde (GA) were used as a control group, and the effect of different types of zeolites on biosensor responses was investigated in detail by using silicalite, zeolite beta (BEA), nano-sized zeolite beta...
Citation Formats
E. Soy et al., “Direct evidence of advantage of using nanosized zeolite Beta for ISFET-based biosensor construction,” JOURNAL OF NANOPARTICLE RESEARCH, pp. 0–0, 2013, Accessed: 00, 2020. [Online]. Available: