RAPID AND COMPREHENSIVE SEPARATION FOR THE PHENOLIC CONSTITUENTS OF QUERCUS BRANTII ACORNS BY RP-HPLC-DAD

Date

2014-04-03

Author

Çoruh, Nursen
Nebigil, Can
Ozgokce, Fevzi

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Quercus brantii Lindley (Fagaceae) is a common oak region of South and East Anatolia. For many civilizations, acorns of Quercus species have been considered as food as well as used in folk-medicine. Phenolic composition of the acorns of Q. brantii was studied by high performance liquid chromatography equipped with a diode array detector. To the best of our knowledge, this is the first study regarding a comprehensive separation and identification of the phenolic constituents of Q. brantii acorns. Rapid separation and identification were performed by the reverse phase HPLC-DAD. Identification of the phenolics was also confirmed by UPLC-MS and when necessary structural characterization was accomplished by NMR and FT-IR techniques. As a result, the phenolic composition of the acorns of Q. brantii were realized as gallic acid, methyl gallate, epi-catechin, p-coumaric acid, ellagic acid, rutin and quercetin in the respective elution order of the RP-HPLC chromatogram.

Subject Keywords

Acorns, Fractionation, HPLC-DAD, Isolation, Phenolic composition, Quercus brantii

URI

https://hdl.handle.net/11511/32803

Journal

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES

DOI

https://doi.org/10.1080/10826076.2012.759126

Collections

Department of Chemistry, Article

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Citation Formats

N. Çoruh, C. Nebigil, and F. Ozgokce, “RAPID AND COMPREHENSIVE SEPARATION FOR THE PHENOLIC CONSTITUENTS OF QUERCUS BRANTII ACORNS BY RP-HPLC-DAD,” JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, pp. 907–915, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32803.