A dye-ligand immobilized poly(2-hydroxyethylmethacrylate) membrane used for adsorption and isolation of immunoglobulin G

Date

2007-05-01

Author

Bayramoglu, Gulay
Öktem, Hüseyin Avni
Arica, M. Yakup

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Poly(2-hydroxyethylmethaerylate) (pHEMA) membrane was prepared by photo-polymerization and a dye ligand (i.e., Reactive Green 5) was immobilised on the membrane surface (pHEMA-RG-5). Surface wettability and hydrophilicity of the dye-ligand immobilised membranes were investigated by static contact angle measurements. The chromatographic properties of the dye-ligand immobilized membrane for adsorption and purification of IgG are discussed using bare membrane as a control system. The non-specific adsorption of IgG was low (0.45 mg protein ml(-1) membrane). The attachment of dye ligand onto the membrane significantly increased the IgG adsorption (33.75 mg protein ml(-1) membrane). The maximum IgG adsorption was observed at pH 6.0. The reversible IgG adsorption on the membrane obeyed both the Freundlich and the Langmuir-Freundlich isotherm models. To test the efficiency of IgG isolation from human serum with the pHEMA-RG-5 membrane, the purity of the eluted IgG, as determined by HPLC, was 81% with a recovery of 67%.

Subject Keywords

Biotechnology, Environmental Engineering, Bioengineering, Biomedical Engineering

URI

https://hdl.handle.net/11511/34723

Journal

BIOCHEMICAL ENGINEERING JOURNAL

DOI

https://doi.org/10.1016/j.bej.2006.11.025

Collections

Department of Biology, Article

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Citation Formats

G. Bayramoglu, H. A. Öktem, and M. Y. Arica, “A dye-ligand immobilized poly(2-hydroxyethylmethacrylate) membrane used for adsorption and isolation of immunoglobulin G,” BIOCHEMICAL ENGINEERING JOURNAL, pp. 147–155, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/34723.