Transcriptome profiling of wheat under heat and cold stress treatments

2012-09-23
BALOĞLU, MEHMET CENGİZ
Oz, M. Tufan
Öktem, Hüseyin Avni
Yucel, Meral
Microarray analysis was performed to indicate effects of cold and heat stress treatments on global gene expression profiles of wheat. Two groups were generated for comparison of microarray data analysis. Cold and heat conditions were separately compared to control condition. The Affymetrix GeneChip® Wheat Genome Array contains 61,127 probe sets designed to target 55,052 wheat transcripts. Out of these more than 60,000 probes, 12,895 (21.5%) were found to be differentially expressed at least p value lower than 0.05 and fold change (FC) higher than 2. Alternation in expression level of about 2550 probe sets was common after the cold and heat stress treatments. Approximately 3600 and 5500 probe sets were differentially expressed after the heat and cold stresses, respectively. Differentially regulated genes show that temperature changes directly affected a large and complex transcriptional network associated with defense, metabolism and development. Genes involved in cold stress-responsive and different cold acclimation proteins were extremely up-regulated upon exposure to cold stress. Both expression levels of small and large sub-unit heat shock proteins significantly increased following heat stress period.
NEW BIOTECHNOLOGY

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Citation Formats
M. C. BALOĞLU, M. T. Oz, H. A. Öktem, and M. Yucel, “Transcriptome profiling of wheat under heat and cold stress treatments,” NEW BIOTECHNOLOGY, pp. 0–0, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/34957.