Transcriptome profiling of wheat under heat and cold stress treatments

Oz, M. Tufan
Öktem, Hüseyin Avni
Yucel, Meral
Microarray analysis was performed to indicate effects of cold and heat stress treatments on global gene expression profiles of wheat. Two groups were generated for comparison of microarray data analysis. Cold and heat conditions were separately compared to control condition. The Affymetrix GeneChip® Wheat Genome Array contains 61,127 probe sets designed to target 55,052 wheat transcripts. Out of these more than 60,000 probes, 12,895 (21.5%) were found to be differentially expressed at least p value lower than 0.05 and fold change (FC) higher than 2. Alternation in expression level of about 2550 probe sets was common after the cold and heat stress treatments. Approximately 3600 and 5500 probe sets were differentially expressed after the heat and cold stresses, respectively. Differentially regulated genes show that temperature changes directly affected a large and complex transcriptional network associated with defense, metabolism and development. Genes involved in cold stress-responsive and different cold acclimation proteins were extremely up-regulated upon exposure to cold stress. Both expression levels of small and large sub-unit heat shock proteins significantly increased following heat stress period.


Transcriptomic analysis of the heat stress response for a commercial baker's yeast Saccharomyces cerevisiae
Varol, Duygu; Purutçuoğlu Gazi, Vilda; YILMAZ, REMZİYE (2018-02-01)
The aim of this study is to explore the effects of heat stresses on global gene expression profiles and to identify the candidate genes for the heat stress response in commercial baker's yeast (Saccharomyces cerevisiae) by using microarray technology and comparative statistical data analyses. The data from all hybridizations and array normalization were analyzed using the GeneSpringGX 12.1 (Agilent) and the R 2.15.2 program language. In the analysis, all required statistical methods were performed comparati...
Transcriptome analysis of thermoplasma volcanium GSS1 under extreme stress
Zabcı, Sema; Kocabıyık, Semra; Department of Biology (2015)
In this study, to provide new insight into anti-stress mechanisms in archaea, we have carried out genome wide transcriptome analysis of Thermoplasma volcanium under severe stress conditions (i.e., heat- shock at 68 ºC, sub-lethal and lethal oxidative stress and pH stress at pH 5.0) using whole genome gene expression microarray. The change in the gene expression profile in response to three stressors indicated retardation of cell growth accompanied by down regulation of genes involved in essential cellular p...
Physiological, Biochemical, and Transcriptomic Responses to Boron Toxicity in Leaf and Root Tissues of Contrasting Wheat Cultivars
Kayihan, Ceyhun; Oz, Mehmet Tufan; EYİDOĞAN, FÜSUN; Yucel, Meral; Öktem, Hüseyin Avni (2017-02-01)
In this study, we examined physiological, biochemical, and transcriptomic responses to toxic boron (B) treatment in leaves and roots of two wheat cultivars in order to gain better insight into B response or tolerance mechanisms. Delayed development and reduced vigor caused by high B were not observed in leaves and roots of both cultivars. Length, wet weight, and dry weight were not markedly changed under B toxicity. In leaves, when compared to control, 995 and 892 genes were significantly expressed at least...
Genes involved in mRNA surveillance are induced in Brachypodium distachyon under cadmium toxicity
Background Cd accumulation in plant cells results in dramatic problems including oxidative stress and inhibition of vital enzymes. It also affects mineral uptakes by disrupting membrane permeability. Interaction among Cd and other plant nutrient elements changes the nutritional contents of crops and reduces their yield. Methods and results In the present study, Cd stress in Brachypodium distachyon led to the upregulation of some heavy metal transport genes (influx or efflux) encoding cation-efflux proteins,...
Transcriptome profiles associated with selenium-deficiency-dependent oxidative stress identify potential diagnostic and therapeutic targets in liver cancer cells
Gozen, Damla; Kahraman, Deniz Cansen; Narci, Kubra; Shehwana, Huma; KONU KARAKAYALI, ÖZLEN; Cetin-Atalay, Rengul (2021-01-01)
Hepatocellular carcinoma (HCC) is one of the most common cancer types with high mortality rates and displays increased resistance to various stress conditions such as oxidative stress. Conventional therapies have low efficacies due to resistance and off-target effects in HCC. Here we aimed to analyze oxidative stress-related gene expression profiles of HCC cells and identify genes that could be crucial for novel diagnostic and therapeutic strategies. To identify important genes that cause resistance to reac...
Citation Formats
M. C. BALOĞLU, M. T. Oz, H. A. Öktem, and M. Yucel, “Transcriptome profiling of wheat under heat and cold stress treatments,” NEW BIOTECHNOLOGY, pp. 0–0, 2012, Accessed: 00, 2020. [Online]. Available: