Physiological, Biochemical, and Transcriptomic Responses to Boron Toxicity in Leaf and Root Tissues of Contrasting Wheat Cultivars

Kayihan, Ceyhun
Oz, Mehmet Tufan
Yucel, Meral
Öktem, Hüseyin Avni
In this study, we examined physiological, biochemical, and transcriptomic responses to toxic boron (B) treatment in leaves and roots of two wheat cultivars in order to gain better insight into B response or tolerance mechanisms. Delayed development and reduced vigor caused by high B were not observed in leaves and roots of both cultivars. Length, wet weight, and dry weight were not markedly changed under B toxicity. In leaves, when compared to control, 995 and 892 genes were significantly expressed at least twofold under B toxicity in Atay and Bolal, respectively. In roots, expressions of 1248 and 957 genes were responsive to B toxicity in Atay and Bolal, respectively. In leaf and root tissues, B toxicity induced more genes related to protein degradation in Atay than those in Bolal. These differences in transcriptome were attributed to higher B accumulation in the sensitive cultivar which required high level of metabolic adjustment. B toxicity stress did not cause any significant change in photosynthetic activity and contents of proline and glycine betaine in both cultivars. Coordinately, we did not find any differentially expressed genes required for proline and glycine betaine metabolisms. Genes related to hormone signaling, kinases, transcription factors such as WRKY and MYB, and key enzymes in reactive oxygen species (ROS) scavenging mechanisms were differentially affected by B toxicity in both cultivars. Among commonly regulated genes in Atay and Bolal, glutathione S-transferase (GST) and NIP4;1 (nodulin-26-like intrinsic proteins) genes stand out as prominent actors in B stress response.


Physiological, biochemical and molecular analysis of wheat cultivars under boron treatments
Kayıhan, Ceyhun; Yücel, Ayşe Meral; Eyidoğan, Füsun; Department of Biotechnology (2014)
Many studies have described the physiological, biochemical and molecular responses to boron (B) toxicity and deficiency individually in plants. However, overall pattern of gene expression changes and their physiological and biochemical significances after high B, low B and supplementing artificial B-enriched fertilizer (Tarımbor) application have not been proposed up till now. In the present study, we assessed global changes in gene expression of two wheat cultivars Bolal-2973 and Atay-85 differing in their ...
Nutritional and cultural parameters influencing antidipteran delta endotoxin production
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In this study, various nutritional and cultural parameters influencing diptera-specific delta-endotoxin synthesis by Bacillus thuringiensis subsp. israelensis HD500 were investigated. Of various inorganic nitrogen sources, the highest yields of Cry11Aa and Cry4Ba proteins were obtained on (NH4)(2)HPO4. Among carbon sources, inulin, dextrin, maltose, lactose, sucrose, whey and glycerol were all stimulatory, while glucose, starch and molasses were suppressive. High concentrations of inorganic phosphate (50 to...
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Kayihan, Ceyhun; Aksoy, Emre; Mutlu, Su Naz (2023-1-01)
Plants activate glutathione (GSH)-dependent detoxification pathways at biochemical and molecular levels under boron (B) toxicity. Sulfate uptake and transport are necessary for GSH biosynthesis in plants. Therefore, the transcriptional regulation of some sulfate transporters was determined in this study to clarify the importance of these transporters in leaf and root tissues of Arabidopsis thaliana under toxic B conditions. The expression level of SULTR1;3 was dramatically increased in leaf and root tissues...
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Evaluation of photosynthetic performance of wheat cultivars exposed to boron toxicity by the JIP fluorescence test
Oz, M. T.; TURAN, ÖZLEM; Kayihan, C.; EYİDOĞAN, FÜSUN; EKMEKÇİ, YASEMİN; YÜCEL, MUSTAFA; Öktem, Hüseyin Avni (Institute of Experimental Botany, 2014-12-01)
The changes in growth and photosynthetic performance of two wheat (Triticum aestivum L.) cultivars (Bolal-2973 and Atay-85) differing in their sensitivity to boron (B) toxicity were investigated under toxic B conditions. Eight-day old seedlings were exposed to highly toxic B concentrations (5, 7.5, and 10 mM H3BO3) for 5 and 9 days. Fast chlorophyll a fluorescence kinetics was determined and analysed using JIP test. Growth parameters, tissue B contents, and membrane damage were measured at two stress durati...
Citation Formats
C. Kayihan, M. T. Oz, F. EYİDOĞAN, M. Yucel, and H. A. Öktem, “Physiological, Biochemical, and Transcriptomic Responses to Boron Toxicity in Leaf and Root Tissues of Contrasting Wheat Cultivars,” PLANT MOLECULAR BIOLOGY REPORTER, pp. 97–109, 2017, Accessed: 00, 2020. [Online]. Available: