Genes involved in mRNA surveillance are induced in Brachypodium distachyon under cadmium toxicity

Aksoy, Emre
Background Cd accumulation in plant cells results in dramatic problems including oxidative stress and inhibition of vital enzymes. It also affects mineral uptakes by disrupting membrane permeability. Interaction among Cd and other plant nutrient elements changes the nutritional contents of crops and reduces their yield. Methods and results In the present study, Cd stress in Brachypodium distachyon led to the upregulation of some heavy metal transport genes (influx or efflux) encoding cation-efflux proteins, heavy metal-associated proteins and NRAMP proteins. The Arabidopsis orthologs of the differentially expressed B. distachyon genes (DEGs) under Cd toxicity were identified, which exhibited Bradi4g26905 was an ortholog of AtALY1-2. Detailed co-expression network and gene ontology analyses found the potential involvement of the mRNA surveillance pathway in Cd tolerance in B. distachyon. These genes were shown to be downregulated by sulfur (S) deficiency. Conclusions This is the first transcriptomic study investigating the effect of Cd toxicity in B. distachyon, a model plant for genomic studies in Poaceae (Gramineae) species. The results are expected to provide valuable information for more comprehensive research related to heavy metal toxicity in plants.


Gene expression profiling of Thermoplasma volcanium under extreme stress conditions
Kocabıyık, Semra (2014-06-26)
Identifying the stress response mechanisms in Archaea mighthave potential applications considering the industrial importanceof their enzymes. In this study, as an extension of our researchon anti-stress mechanisms, we have carried out genome widetranscriptome analysis ofThermoplasma volcanium(grows opti-mally at pH 2.7 and 60◦C) under extreme stress (i.e., heat- shockat 68◦C, hydrogen peroxide, 0.03 mM H2O2and pH stress atpH 5.0) using AgilentCustom Gene Expression Microarray.The data w...
Regulation and role of CCAAT/enhancer binding protein during differentiation of intestinal epithelial cells
Memişoğlu, Aslı Sade; Banerjee, Sreeparna (2013-09-01)
Background: The molecular mechanisms of differentiation of intestinal epithelial cells, is poorly understood and disruption of this balance may result in neoplastic transformation and malignant growth. The family of CCAAT/Enhancer Binding Protein (C/EBP) transcription factors is implicated in cellular growth, differentiation, inflammation and development and control differentiation in several cell types. The involvement of these transcription factors in intestinal differentiation is not known. The aim of th...
Systems-level analysis reveals multiple modulators of epithelial-mesenchymal transition and identifies DNAJB4 and CD81 as novel metastasis inducers in breast cancer
Kagiali, Zeynep Cansu Üretmen; Sanal, Erdem; Karayel, Özge; Polat, Ayşe Nur; Saatçi, Özge; Ersan, Pelin Gülizar; Trappe, Kathrin; Renard, Bernhard Y.; Önder, Tamer T.; Tunçbağ, Nurcan; Şahin, Özgür; Özlü, Nurhan (American Society for Biochemistry & Molecular Biology (ASBMB), 2019-09)
Epithelial-mesenchymal transition (EMT) is driven by complex signaling events that induce dramatic biochemical and morphological changes whereby epithelial cells are converted into cancer cells. However, the underlying molecular mechanisms remain elusive. Here, we used mass spectrometry based quantitative proteomics approach to systematically analyze the post-translational biochemical changes that drive differentiation of human mammary epithelial (HMLE) cells into mesenchymal. We identified 314 proteins out...
Expression System for Recombinant Human Growth Hormone Production from Bacillus subtilis
ÖZDAMAR, HASAN TUNÇER; Sentuerk, Birguel; Yilmaz, Oezge Deniz; Calik, Guezide; Celik, Eda; Çalık, Pınar (2009-01-01)
We demonstrate for the first time, an expression system mimicking serine alkaline protease synthesis and secretion, producing native form of human growth hormone (hGH) from Bacillus subtilis. A hybrid-gene of two DNA fragments, i.e., signal (pre-) DNA sequence of B. licheniformis serine alkaline protease gene (subC) and cDNA encoding hGH, were cloned into pMK4 and expressed under deg-promoter in B. subtilis. Recombinant-hGH (rhGH) produced by B. subtilis carrying pMK4::pre(subC)::hGH was secreted. N-termina...
Transcriptome analysis of thermoplasma volcanium GSS1 under extreme stress
Zabcı, Sema; Kocabıyık, Semra; Department of Biology (2015)
In this study, to provide new insight into anti-stress mechanisms in archaea, we have carried out genome wide transcriptome analysis of Thermoplasma volcanium under severe stress conditions (i.e., heat- shock at 68 ºC, sub-lethal and lethal oxidative stress and pH stress at pH 5.0) using whole genome gene expression microarray. The change in the gene expression profile in response to three stressors indicated retardation of cell growth accompanied by down regulation of genes involved in essential cellular p...
Citation Formats
E. Aksoy, E. FİLİZ, Ş. ORMAN, D. ÇETİN, and M. A. AKBUDAK, “Genes involved in mRNA surveillance are induced in Brachypodium distachyon under cadmium toxicity,” MOLECULAR BIOLOGY REPORTS, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: