Genes involved in mRNA surveillance are induced in Brachypodium distachyon under cadmium toxicity

Aksoy, Emre
Background Cd accumulation in plant cells results in dramatic problems including oxidative stress and inhibition of vital enzymes. It also affects mineral uptakes by disrupting membrane permeability. Interaction among Cd and other plant nutrient elements changes the nutritional contents of crops and reduces their yield. Methods and results In the present study, Cd stress in Brachypodium distachyon led to the upregulation of some heavy metal transport genes (influx or efflux) encoding cation-efflux proteins, heavy metal-associated proteins and NRAMP proteins. The Arabidopsis orthologs of the differentially expressed B. distachyon genes (DEGs) under Cd toxicity were identified, which exhibited Bradi4g26905 was an ortholog of AtALY1-2. Detailed co-expression network and gene ontology analyses found the potential involvement of the mRNA surveillance pathway in Cd tolerance in B. distachyon. These genes were shown to be downregulated by sulfur (S) deficiency. Conclusions This is the first transcriptomic study investigating the effect of Cd toxicity in B. distachyon, a model plant for genomic studies in Poaceae (Gramineae) species. The results are expected to provide valuable information for more comprehensive research related to heavy metal toxicity in plants.


Gene expression profiling of Thermoplasma volcanium under extreme stress conditions
Kocabıyık, Semra (2014-06-26)
Identifying the stress response mechanisms in Archaea mighthave potential applications considering the industrial importanceof their enzymes. In this study, as an extension of our researchon anti-stress mechanisms, we have carried out genome widetranscriptome analysis ofThermoplasma volcanium(grows opti-mally at pH 2.7 and 60◦C) under extreme stress (i.e., heat- shockat 68◦C, hydrogen peroxide, 0.03 mM H2O2and pH stress atpH 5.0) using AgilentCustom Gene Expression Microarray.The data w...
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Memişoğlu, Aslı Sade; Banerjee, Sreeparna (2013-09-01)
Background: The molecular mechanisms of differentiation of intestinal epithelial cells, is poorly understood and disruption of this balance may result in neoplastic transformation and malignant growth. The family of CCAAT/Enhancer Binding Protein (C/EBP) transcription factors is implicated in cellular growth, differentiation, inflammation and development and control differentiation in several cell types. The involvement of these transcription factors in intestinal differentiation is not known. The aim of th...
Expression System for Recombinant Human Growth Hormone Production from Bacillus subtilis
ÖZDAMAR, HASAN TUNÇER; Sentuerk, Birguel; Yilmaz, Oezge Deniz; Calik, Guezide; Celik, Eda; Çalık, Pınar (2009-01-01)
We demonstrate for the first time, an expression system mimicking serine alkaline protease synthesis and secretion, producing native form of human growth hormone (hGH) from Bacillus subtilis. A hybrid-gene of two DNA fragments, i.e., signal (pre-) DNA sequence of B. licheniformis serine alkaline protease gene (subC) and cDNA encoding hGH, were cloned into pMK4 and expressed under deg-promoter in B. subtilis. Recombinant-hGH (rhGH) produced by B. subtilis carrying pMK4::pre(subC)::hGH was secreted. N-termina...
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Zabcı, Sema; Kocabıyık, Semra; Department of Biology (2015)
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Expression Analysis of TaNAC69-1 and TtNAMB-2, Wheat NAC Family Transcription Factor Genes Under Abiotic Stress Conditions in Durum Wheat (Triticum turgidum)
Baloglu, Mehmet Cengiz; Oz, Mehmet Tufan; Öktem, Hüseyin Avni; Yücel, Ayşe Meral (2012-10-01)
NAC-type plant-specific transcription factor genes encode proteins that play important roles in abiotic stress responses, as well as regulation of plant development. In the current study, expression profiles of wheat NAC-type transcription factor genes, TaNAC69-1 and TtNAMB-2, were examined under drought, salt, cold, and heat stress conditions in wheat. Based on reverse transcription quantitative PCR results, TaNAC69-1 was strongly expressed under drought, salinity, and high-temperature stress conditions. C...
Citation Formats
E. Aksoy, E. FİLİZ, Ş. ORMAN, D. ÇETİN, and M. A. AKBUDAK, “Genes involved in mRNA surveillance are induced in Brachypodium distachyon under cadmium toxicity,” MOLECULAR BIOLOGY REPORTS, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: