A High Throughput Lab-On-A-Chip System for Label Free Quantification of Breast Cancer Cells under Continuous Flow

Yalçın, Yasemin
Ozgur, E.
Gündüz, Ufuk
Eminoglu, S.
Külah, Haluk
Akın, Tayfun
This paper presents an LOC system combining microfluidic DEP channel with a CMOS image sensor for label and lens free detection and real-time counting of MCF-7 cells under continuous flow. Trapped and then released MCF-7 cells are accurately detected and counted under flow with a CMOS image sensor integrated underneath the DEP channel, for the first time in the literature. CMOS image sensor can capture 391 frames per second (fps) that allows detection of the released cells flowing through the channel with a flow rate up to 130 mu l/min (0.468 m/s). Therefore, the proposed system is able to detect the cells under high flow where conventional techniques for cell quantification such as fluorescent tagging become unusable. Detected cells are automatically counted with a computer program and the counting accuracy of the whole system is 95%. (C) 2016 The Authors. Published by Elsevier Ltd.


A CMOS visible image sensor array using current mirroring integration readout circuitry
Akbay, Selim Sermet; Bircan, A.; Akın, Tayfun (null; 2000-08-30)
This paper reports the development of a CMOS visible sensor array using a high performance readout circuit called Current Mirroring Integration (CMI). The sensor element is a photodiode implemented using n-well and p+ -active layers available in any CMOS process. The current generated by optical excitation is mirrored and integrated in an off-pixel capacitor using the CMI readout circuit, which provides high injection efficiency, low input impedance, almost-zero and stable detector bias, and a high dynamic ...
A low cost uncooled infrared microbolometer focal plane array using the CMOS n-well layer
Tezcan, DS; Eminoglu, S; Akar, OS; Akın, Tayfun (2001-01-25)
This paper reports a low-cost, 256-pixel uncooled infrared microbolometer focal plane array (FPA) implemented using a 0.8 mum CMOS process where the n-well layer is used as the active microbolometer material. The suspended n-well structure is obtained by simple front-end bulk etching of the fabricated CMOS dies, while the n-well region is protected from etching by electrochemical etch-stop technique within a TMAH solution. Electrical connections to the suspended n-well are obtained with polysilicon intercon...
A dielectrophoretic cell/particle separator fabricated by spiral channels and concentric gold electrodes
Yilmaz, G.; Çiftlik, A.T.; Külah, Haluk (2009-12-14)
This paper presents the design and implementation of a novel dielectrophoresis (DEP) system with spiral channels and concentric electrodes for high resolution cell separation applications. The device is fabricated with a 4 mask parylene process and the design is optimized in MATLAB Simulink reg to confine the operation. Tests with micro particles of different sizes are performed to show size-based separation by dielectrophoresis. Proposed device is also tested with K562 leukemia cell lines to prove that the...
Capturing and detection of MCF-7 breast cancer cells with a CMOS image sensor
Musayev, Javid; Altiner, Caglar; Adiguzel, Yekbun; Külah, Haluk; Eminoglu, Selim; Akın, Tayfun (2014-08-15)
This paper presents a CMOS image sensor with a 32 x 32 pixel array for cell capture, detection, and quantification. Pixels measuring 15 pm x 15 mu m have a modified structure, suitable for post-CMOS electroless gold plating, which enables surface activation for cell capture without the need for any intermediate layer. This structure also increases the detection probability of captured cells, even when cells are much smaller than the pixel, owing to a special light mask implemented on pixels. Cells as small ...
A low-cost small pixel uncooled infrared detector for large focal plane arrays using a standard CMOS process
Eminoglu, S; Tanrikulu, MY; Tezcan, DS; Akın, Tayfun (2002-04-03)
This paper reports the development of a low-cost, small pixel uncooled infrared detector using a standard CMOS process. The detector is based on a suspended and thermally isolated p(+)-active/n-well diode whose forward voltage changes due to an increase in the pixel temperature with absorbed infrared radiation. The detector is obtained with simple post-CMOS etching steps on dies fabricated using a standard n-well CMOS process. The post-CMOS process steps are achieved without needing any deposition or lithog...
Citation Formats
M. Y. ASLAN et al., “A High Throughput Lab-On-A-Chip System for Label Free Quantification of Breast Cancer Cells under Continuous Flow,” 2016, vol. 27, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/35133.