Capturing and detection of MCF-7 breast cancer cells with a CMOS image sensor

2014-08-15
Musayev, Javid
Altiner, Caglar
Adiguzel, Yekbun
Külah, Haluk
Eminoglu, Selim
Akın, Tayfun
This paper presents a CMOS image sensor with a 32 x 32 pixel array for cell capture, detection, and quantification. Pixels measuring 15 pm x 15 mu m have a modified structure, suitable for post-CMOS electroless gold plating, which enables surface activation for cell capture without the need for any intermediate layer. This structure also increases the detection probability of captured cells, even when cells are much smaller than the pixel, owing to a special light mask implemented on pixels. Cells as small as 3 pm in diameter can be detected with this pixel structure. The proof of concept for surface activation of the gold coated sensor pixels was demonstrated by capturing and imaging MCF-7 breast cancer cells on the modified sensor surface. Accordingly, electroless gold coating of the Al pixels was achieved and the gold coated surface of the CMOS image sensor was activated with thiol-modified antibodies that can capture MCF-7 cells. Cells were then optically detected under green LED illumination.
SENSORS AND ACTUATORS A-PHYSICAL

Suggestions

Replication of high aspect ratio pillar array structures in biocompatible polymers for tissue engineering applications
Padeste, C.; Ozcelik, H.; Ziegler, J.; Schleunitz, A.; Bednarzik, M.; Yucel, D.; Hasırcı, Vasıf Nejat (2011-08-01)
We developed a simple two-step replication method to transfer arrays of high aspect ratio nanopillars into films of poly(L-D,L-lactic acid) (PLLA). Such structures are promising model surfaces for tissue engineering applications. From arrays of 1 mu m high and 200 nm wide pillars produced with e-beam lithography and reactive ion etching negative replicas were first formed by polydimethylsiloxane (PDMS) casting. The final replicates were produced by solvent casting from 1% to 4% solutions of PLLA in chlorina...
A High Throughput Lab-On-A-Chip System for Label Free Quantification of Breast Cancer Cells under Continuous Flow
ASLAN, MUSTAFA YASİN; Yalçın, Yasemin; Ozgur, E.; Gündüz, Ufuk; Eminoglu, S.; Külah, Haluk; Akın, Tayfun (Elsevier BV; 2016-05-27)
This paper presents an LOC system combining microfluidic DEP channel with a CMOS image sensor for label and lens free detection and real-time counting of MCF-7 cells under continuous flow. Trapped and then released MCF-7 cells are accurately detected and counted under flow with a CMOS image sensor integrated underneath the DEP channel, for the first time in the literature. CMOS image sensor can capture 391 frames per second (fps) that allows detection of the released cells flowing through the channel with a...
Cell detection using a CMOS image sensor with modified pixel structure suitable for bio-chemical surface activation
Musayev, Javid; Adiguzel, Yekbun; Külah, Haluk; Akın, Tayfun (2013-04-02)
This paper presents a CMOS image sensor with a 32 x 32 pixel array suitable for cell capture, detection, and quantification. Pixels measuring 15 mu m x 15 mu m have a modified structure, suitable for post-CMOS electroless gold plating, which enables surface activation for cell capture without the need for any intermediate layer. This structure also increases the detection probability of captured cells as opposed to non-captured ones, owing to a special light mask (metal shield) implemented on pixels. The li...
Broadband absorption enhancement in an uncooled microbolometer infrared detector
Kebapci, Basak; Dervisoglu, Ozgecan; Battal, Enes; OKYAY, Ali Kemal; Akın, Tayfun (2014-05-08)
This paper introduces a method for a broadband absorption enhancement in the LWIR range (8-12 µm), in single layer microbolometer pixels with 35 μm pitch. For the first time in the literature, this study introduces a very simple and low cost approach to enhance the absorption by embedding plasmonic structures at the same level as the already existing metallic layer of a microbolometer pixel. The metal layer comprises the electrode and the arm structures on the body. Even though the periodicity of the plasmo...
Radial Heterojunction Solar Cell Consisting of n-Type Rutile Nanowire Arrays Infiltrated by p-Type CdTe
Hosseini, Arezoo; Kar, Piyush; Hsieh, Ling-Hsuan; Wiltshire, Benjamin D.; Mohammadpour, Arash; Farsinezhad, Samira; Benlamri, Mourad; Zhang, Yun; Ercelebi, C.; Shankar, Karthik (2017-07-01)
We report a p-n heterojunction solid-state solar cell comprised of p-type CdTe that was electrochemically deposited onto hydrothermally synthesized vertically oriented arrays of n-type single crystal rutile TiO2 nanowire arrays (RNWAs). The nanowires were grown on fluorine doped tin oxide (FTO)-coated transparent conductive glass substrates by hydrothermal synthesis. Morphological studies revealed the TiO2 nanowires to be completely infiltrated by the CdTe. The solid-state solar cell exhibited an open circu...
Citation Formats
J. Musayev, C. Altiner, Y. Adiguzel, H. Külah, S. Eminoglu, and T. Akın, “Capturing and detection of MCF-7 breast cancer cells with a CMOS image sensor,” SENSORS AND ACTUATORS A-PHYSICAL, pp. 105–114, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/35461.