Analysis of nanoparticle Transmission Electron Microscopy data using a public-domain image-processing program, Image

Date

2006-01-01

Author

Woehrle, GH
Hutchison, JE
Özkar, Saim
Finke, RG

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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The need to easily and quickly count larger numbers of nanoparticles, in order to obtain statistically useful size and size-distribution data, is addressed via the use of a readily available, free, public-domain program for particle counting, NIH-Image (and 2 others derived from it, Scion Image and Image J), collectively referred to herein as Image. The best protocols that we have found useful for the use of Image are reported; both appropriate as well as problematic applications of Image are then illustrated with a series of TEM images of Ir(0), Pd(0) and Au(0) nanoclusters. Methods to detect and image nanoclusters with sub-1-nm core diameters are reported and illustrated in the literature with An nanoclusters, an important problem since the literature indicates that subnanometer An nanoclusters are often present, but undetected. A list of suggestions and caveats for the appropriate use of Image is also provided, since this contribution is directed at first-time users who are not presently using nanoparticle imaging software. The reader is also reminded of several, well-known caveats for the use of TEM in obtaining size data for nanostructures. Overall, Image is a free, public-domain program that is useful for the rapid counting of large numbers of particles.

Subject Keywords

Nanoparticles, Particle size distributions, Transmission electron microscopy, Image analysis, Image (NIH-Image, Scion Image, Image J), Histograms

URI

https://hdl.handle.net/11511/55747

Journal

TURKISH JOURNAL OF CHEMISTRY

Collections

Department of Chemistry, Article

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Citation Formats

G. Woehrle, J. Hutchison, S. Özkar, and R. Finke, “Analysis of nanoparticle Transmission Electron Microscopy data using a public-domain image-processing program, Image,” TURKISH JOURNAL OF CHEMISTRY, pp. 1–13, 2006, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/55747.