Cellular uptake and apoptotic potential of rhenium labeled magnetic protein cages in MDA-MB-231 cells

Asik, Elif
Aslan, Tugba Nur
Güray, Nülüfer Tülün
Volkan, Mürvet
Re-188-magnetoferritin nanoparticles (NPs) provide an attractive platform for localized radiation therapy due to their magnetic targeting capability while enhancing contrast in magnetic resonans imaging (MRI) signals. In this study, cellular uptake, in vitro cytotoxicity, apoptotic potential of a non-radioactive isotope of rhenium in the form of Re-187-magnetoferritin NPs were evaluated in both human normal mammary epithelial and breast metastatic adenocarcinoma cell lines. The results showed that, NP administration into the cells is through receptor mediated endocytosis and cancer cells displayed significantly higher uptake and cytotoxicity compared to normal cells. IC50 values of nanoparticles were calculated as 0.96 mg/mL for cancer and 1.73 mg/mL for normal cells. Annexin V/ Propidium Iodide (PI) staining also showed that, NPs induced higher apoptotic rates in cancer cells compared to normal cells. Gene expression analyses confirming the results showed that, pro-apoptotic PUMA and BAX genes were significantly up-regulated while anti-apoptotic BCL-2 and SURVIVIN genes were down-regulated in cancer cells compared to normal cells. Overall, these in vitro results suggest that, Re-187-magnetoferritin NPs have a promising potential for cancer therapy and can be used for imaging and diagnostic purposes for breast cancer at concentrations lower than 0.96 mg/mL. At concentrations above 1 mg/mL, NPs induce apoptosis which can also be used for cancer treatments.


Cellular uptake, genotoxicity and cytotoxicity of cobalt ferrite magnetic nanoparticles in human breast cells
Asik, Elif; Akpinar, Yeliz; Güray, Nülüfer Tülün; Iscan, Mesude; ÇAKMAK DEMİRCİGİL, GONCA; Volkan, Mürvet (Oxford University Press (OUP), 2016-01-01)
Magnetic nanoparticles (MNPs) have been increasingly used for many years as MRI agents and for gene delivery and hyperthermia therapy, although there have been conflicting results on their safety. In this study, cobalt ferrite magnetic nanoparticles (CoFe-MNPs) were prepared by the co-precipitation method and their surfaces were modified with silica by the sol-gel method. The particle and hydrodynamic sizes, morphology and crystal structure of the bare and silica-coated CoFe-MNPs were evaluated by transmiss...
Effect of mercury, cadmium, nickel, chromium and zinc on kinetic properties of NADPH-cytochrome P450 reductase purified from leaping mullet (Liza saliens)
Bozcaarmutlu, Azra; Arinc, Emel (Elsevier BV, 2007-04-01)
Information on the mechanism of metal ion inhibition of NADPH-cytochrome P450 reductase is limited. The purpose of the present paper was to elucidate in vitro effect of Hg(+2), Cd(+2), Ni(+2), Cr(+3) and Zn(+2) ions on the purified mullet NADPH-cytochrome P450 reductase. NADPH-cytochrome P450 reductase was purified from detergent-solubilized liver microsomes from leaping mullet (Liza saliens). All of the metal ions caused inhibition of the enzyme activity except Zn(+2). At 50 mu M metal concentration, Hg(+2...
Coil sensitivity map calculation using biot-savart law at 3 tesla and parallel imaging in MRI
Esin, Yunus Emre; Alpaslan, Ferda Nur; Department of Computer Engineering (2017)
Coil spatial sensitivity map is considered as one of the most valuable data used in parallel magnetic resonance imaging (MRI) reconstruction. In this study, a novel sensitivity map extraction method is introduced for phased-array coils. Proposed technique uses Biot-Savart law with coil shape information and low-resolution phase image data to form sensitivity maps. The performance of this method has been tested in the parallel image reconstruction task using sensitivity encoding technique. In MRI, coil sensi...
Forward problem solution of electromagnetic source imaging using a new BEM formulation with high-order elements
Gençer, Nevzat Güneri (IOP Publishing, 1999-09-01)
Representations of the active cell populations on the cortical surface via electric and magnetic measurements are known as electromagnetic source images (EMSIs) of the human brain. Numerical solution of the potential and magnetic fields for a given electrical source distribution in the human brain is an essential part of electromagnetic source imaging. In this study, the performance of the boundary element method (BEM) is explored with different surface element types. A new BEM formulation is derived that m...
Electrical impedance tomography using the magnetic field generated by injected currents
Birgul, O; Ider, YZ (1996-11-03)
In 2D EIT imaging, the internal distribution of the injected currents generate a magnetic field in the imaging region which can be measured by magnetic resonance imaging techniques. This magnetic field is perpendicular to the imaging region on the imaging region and it can be used in reconstructing the conductivity distribution inside the imaging region. For this purpose, internal current distribution is found using the finite element method. The magnetic fields due to this current is found using Biot-Savar...
Citation Formats
E. Asik, T. N. Aslan, N. T. Güray, and M. Volkan, “Cellular uptake and apoptotic potential of rhenium labeled magnetic protein cages in MDA-MB-231 cells,” ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, pp. 127–134, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/35516.