Glycomic Analysis of Tear and Saliva in Ocular Rosacea Patients: The Search for a Biomarker

Download

index.pdf

Date

2012-07-01

Author

Vieira, Ana Carolina
An, Hyun Joo
Özcan Kabasakal, Süreyya
Kim, Jae-Han
Lebrilla, Carlito B.
Mannis, Mark J.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

99
views

73
downloads

The purpose of this study was to study changes in glycosylation in tear and saliva obtained from control and ocular rosacea patients in order to identify potential biomarkers for rosacea. Tear fluid was collected from 51 subjects (28 healthy controls and 23 patients with ocular rosacea). Saliva was collected from 42 of the same subjects (25 controls and 17 patients). Pooled and individual samples were examined to determine overall glycan profiles and individual variations in glycosylation. O-and N- glycans were released from both patients and control subjects. Released glycans were purified and enriched by solid-phase extraction (SPE) with graphitized carbon. Glycans were eluted based on glycan size and polarity. SPE fractions were then analyzed by high-resolution mass spectrometry. Glycan compositions were assigned by accurate masses. Their structures were further elucidated by tandem mass spectrometric using collision-induced dissociation (CID), and specific linkage information was obtained by exoglycosidase digestion. N- and O-glycans were released from 20-mu L samples without protein identification, separation, and purification. Approximately 50 N-glycans and 70 O-glycans were globally profiled by mass spectrometry. Most N-glycans were highly fucosylated, while 0-glycans were sulfated. Normal tear fluid and saliva contain highly fucosylated glycans. The numbers of sulfated glycans were dramatically increased in tear and saliva of rosacea patients compared to controls. Glycans found in tear and saliva from roseatic patients present highly quantitative similarity. The abundance of highly fucosylated N-glycans in the control samples and sulfated O-glycans in ocular rosacea patient samples may lead to the discovery of an objective diagnostic marker for the disease.

Subject Keywords

Biomarkers, Fucosylation, Glycans, Glycomic analysis, Mucin, Ocular rosacea, Oligosaccharides, Tear, Saliva

URI

https://hdl.handle.net/11511/35547

Journal

OCULAR SURFACE

DOI

https://doi.org/10.1016/j.jtos.2012.04.003

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Multidrug resistance mechanisms in imatinib resistant human chronic myeloid leukemia cells Baran, Yusuf; Gündüz, Ufuk; Department of Biology (2006) In this study, mechanisms of resistance to Imatinib-induced apoptosis in human K562 and Meg-1 chronic myeloid leukemia (CML) cells were examined. Continuous exposure of cells to step-wise increasing concentrations of Imatinib resulted in the selection of 0.2 and 1 ́M İmatinib resistant cells. Measurement of endogenous ceramide levels showed that treatment with Imatinib increased the generation of C18-ceramide significantly, which is mainly synthesized by the human longevity assurance gene 1 (hLASS1), in sen...
Characterization of Novel O-Glycans Isolated from Tear and Saliva of Ocular Rosacea Patients Özcan Kabasakal, Süreyya; Vieira, Ana C.; Park, Gun Wook; Kim, Jae Han; Mannis, Mark J.; Lebrilla, Carlito B. (American Chemical Society (ACS), 2013-03-01) O-Glycans in saliva and tear isolated from patients suffering from ocular rosacea, a form of inflammatory ocular surface disease, were profiled, and their structures were elucidated using high resolution mass spectrometry. We have previously shown that certain structures, particularly sulfated oligosaccharides, increased in the tear and saliva of rosacea patients In this study, the structures of these glycans were elucidated using primarily tandem mass spectrometry. There were important similarities in the ...
Effects of early postnatal alcohol exposure on the developing retinogeniculate projections in C57BL/6 mice DURSUN, İLKNUR; Jakubowska-Dogru, Ewa; Elibol-Can, Birsen; van der List, Deborah; Chapman, Barbara; Qi, Lihong; Berman, Robert F. (Elsevier BV, 2013-05-01) Previous studies on the adverse effects of perinatal exposure to ethanol (EtOH) on the developing visual system mainly focused on retinal and optic nerve morphology. The aim of the present study was to investigate whether earlier reported retinal and optic nerve changes are accompanied by anomalies in eye-specific fiber segregation in the dorsal lateral geniculate nucleus (dLGN). C57BL/6 mice pups were exposed to ethanol by intragastric intubation at either 3 or 4 g/kg from postnatal days (PD) 3-10, the thi...
Biocompatibility of Dead Sea Water and retinyl palmitate carrying poly(3-hydroxybutyrate-co-3-hydroxyvalerate) micro/nanoparticles designed for transdermal skin therapy Eke, Gozde; Goni-de-Cerio, Felipe; Suarez-Merino, Blanca; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat (2015-09-01) In this study, novel drug carriers were developed for the treatment of skin conditions such as psoriasis, aging, or ultraviolet damage using micro/nanocapsules and micro/nanospheres of poly(3-hydroxybutyrate-co-3-hydroxyvalerate). The sizes of the particles were in the micron range and were loaded with retinyl palmitate and Dead Sea Water. In some tests, MgCl2 was used as a substitute for Dead Sea Water for accurate determination of released ions of Dead Sea Water. Encapsulation efficiency and loading of wa...
Retinal pigment epithelium cell culture on surface modified poly(hydroxybutyrate-co-hydroxyvalerate) thin films Tezcaner, Ayşen; Hasırcı, Vasıf Nejat (2003-01-01) There is currently no effective treatment for the retinal disorders caused by retinal pigment epithelium (RPE) degeneration. Transplantation of allografts is the main strategy towards correction of this malady. Tissue engineering could offer hope and involve the use of biodegradable polymeric templates to replace diseased or lost RPE. In this study PHBV8 film was chosen as a temporary substrate for growing retinal pigment epithelium cells as an organized monolayer before their subretinal transplantation. Th...

Citation Formats

A. C. Vieira, H. J. An, S. Özcan Kabasakal, J.-H. Kim, C. B. Lebrilla, and M. J. Mannis, “Glycomic Analysis of Tear and Saliva in Ocular Rosacea Patients: The Search for a Biomarker,” OCULAR SURFACE, pp. 184–192, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/35547.