MTA-1 expression is associated with metastasis and epithelial to mesenchymal transition in colorectal cancer cells

Date

2013-04-01

Author

Cagatay, Seda Tuncay
Cimen, Ismail
SAVAŞ, BERNA
Banerjee, Sreeparna

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

176
views

0
downloads

Although metastasis associated protein 1 (MTA1) has been widely linked to tumor metastasis, the relevant mechanisms remain to be elucidated, especially in colorectal cancer (CRC). Here, we have investigated the link between MTA1, metastasis and epithelial-mesenchymal transition (EMT) in CRC. Eighteen normal colon tissues and 91 resected tumor samples were analyzed for MTA1 expression by immunohistochemistry (IHC). IHC indicated low or no nuclear MTA1 expression in the normal tissues and significantly higher expression in Grade II, Grade III and liver metastasis tumors. No statistically significant difference was observed in MTA1 expression between Grade III and liver metastatic tumors. To demonstrate the functional importance of MTA1 in vitro, the gene was silenced in HCT-116 cells and LoVo cells and overexpressed in HCT-116 cells. MTA1 overexpression in HCT-116 cells enhanced proliferation, adhesion to fibronectin, motility, migration, invasion through Matrigel, anchorage-independent growth, neoangiogenesis and induced a loss of apoptosis. Silencing of MTA1 resulted in a reversal of all of these features. Mechanistically, MTA1 silencing caused an increase in the epithelial markers E-cadherin and ZO-1 and a decrease in the mesenchymal marker vimentin while MTA1 overexpression caused an increase in vimentin expression. Moreover, MTA1 enhanced the expression of Snai1 and Slug; silencing of MTA1 reduced their recruitment to the promoter of E-cadherin, thereby leading to its expression. MTA1 is highly expressed in higher grade tumors and is important in the orchestration of various phenotypic changes in CRC, most likely by inducing EMT. This further corroborates its role as a master regulator in tumorigenesis.

Subject Keywords

General Medicine

URI

https://hdl.handle.net/11511/35790

Journal

TUMOR BIOLOGY

DOI

https://doi.org/10.1007/s13277-013-0662-x

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

CpG ODN Nanorings Induce IFN alpha from Plasmacytoid Dendritic Cells and Demonstrate Potent Vaccine Adjuvant Activity Gungor, Bilgi; Yagci, Fuat Cem; Tincer, Gizem; Bayyurt, Banu; Alpdundar, Esin; Yildiz, Soner; Ozcan, Mine; GÜRSEL, İHSAN; Gürsel, Mayda (American Association for the Advancement of Science (AAAS), 2014-05-07) CpG oligodeoxynucleotides (ODN) are short single-stranded synthetic DNA molecules that activate the immune system and have been found to be effective for preventing and treating infectious diseases, allergies, and cancers. Structurally distinct classes of synthetic ODN expressing CpG motifs differentially activate human immune cells. K-type ODN (K-ODN), which have progressed into human clinical trials as vaccine adjuvants and immunotherapeutic agents, are strong activators of B cells and trigger plasmacytoi...
Apolipoprotein A5 polymorphisms in Turkish population: association with serum lipid profile and risk of ischemic stroke CAN DEMİRDÖĞEN, BİRSEN; Sahin, Esra; Ozcelik, Aysun Turkanoglu; Bek, Semai; Demirkaya, Seref; Adalı, Orhan (Springer Science and Business Media LLC, 2012-12-01) Atherosclerosis, a major cause of ischemic stroke, may be associated with variability of triglyceride (TG) levels. Apolipoprotein A5 (APOA5) genetic polymorphisms are associated with altered TG levels. The objective of this study was to investigate the coding region polymorphisms S19W (rs3135506) and G185C (rs2075291) and the promoter region polymorphism -1131T > C (rs662799) of the APOA5 gene as risk factors for ischemic stroke in Turkish population. Study group consisted of 272 ischemic stroke patients an...
Isomer-specific chromatographic profiling yields highly sensitive and specific potential N-glycan biomarkers for epithelial ovarian cancer Hua, Serenus; Williams, Cynthia C.; Dimapasoc, Lauren M.; Ro, Grace S.; Özcan Kabasakal, Süreyya; Miyamoto, Suzanne; Lebrilla, Carlito B.; An, Hyun Joo; Leiserowitzf, Gary S. (Elsevier BV, 2013-03-01) Aberrant glycosylation has been observed for decades in essentially all types of cancer, and is now well established as an indicator of carcinogenesis. Mining the glycome for biomarkers, however, requires analytical methods that can rapidly separate, identify, and quantify isomeric glycans. We have developed a rapid-throughput method for chromatographic glycan profiling using microfluidic chip-based nanoflow liquid chromatography (nano-LC)/mass spectrometry. To demonstrate the utility of this method, we ana...
Epigenetic Mechanisms Underlying the Dynamic Expression of Cancer-Testis Genes, PAGE2, -2B and SPANX-B, during Mesenchymal-to-Epithelial Transition Yilmaz-Ozcan, Sinem; Sade, Asli; Kucukkaraduman, Baris; Kaygusuz, Yasemin; Senses, Kerem Mert; Banerjee, Sreeparna; GÜRE, ALİ OSMAY (Public Library of Science (PLoS), 2014-09-17) Cancer-testis (CT) genes are expressed in various cancers but not in normal tissues other than in cells of the germline. Although DNA demethylation of promoter-proximal CpGs of CT genes is linked to their expression in cancer, the mechanisms leading to demethylation are unknown. To elucidate such mechanisms we chose to study the Caco-2 colorectal cancer cell line during the course of its spontaneous differentiation in vitro, as we found CT genes, in particular PAGE2, -2B and SPANX-B, to be up-regulated duri...
Paraoxonase/arylesterase ratio, PON1 192Q/R polymorphism and PON1 status are associated with increased risk of ischernic stroke Demirdogen, Birsen Can; Turkanoglu, Aysun; Bek, Semai; Sanisoglu, Yavuz; Demirkaya, Seref; Vural, Okay; Arinc, Emel; Adalı, Orhan (Elsevier BV, 2008-01-01) Objectives: In recent years, importance of enzyme activity measurements, in addition to genotyping, in epidemiological studies relating paraoxonase 1 (PON1) and vascular disease was emphasized. This is the first report evaluating paraoxonase and arylesterase activities as risk factors for ischemic stroke. In addition, PON1 192Gln(Q)/Arg(R) and 55Leu(L)/Met(M) polymorphisms were also analyzed.

Citation Formats

S. T. Cagatay, I. Cimen, B. SAVAŞ, and S. Banerjee, “MTA-1 expression is associated with metastasis and epithelial to mesenchymal transition in colorectal cancer cells,” TUMOR BIOLOGY, pp. 1189–1204, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/35790.