Isomer-specific chromatographic profiling yields highly sensitive and specific potential N-glycan biomarkers for epithelial ovarian cancer

Download

index.pdf

Date

2013-03-01

Author

Hua, Serenus
Williams, Cynthia C.
Dimapasoc, Lauren M.
Ro, Grace S.
Özcan Kabasakal, Süreyya
Miyamoto, Suzanne
Lebrilla, Carlito B.
An, Hyun Joo
Leiserowitzf, Gary S.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

175
views

96
downloads

Aberrant glycosylation has been observed for decades in essentially all types of cancer, and is now well established as an indicator of carcinogenesis. Mining the glycome for biomarkers, however, requires analytical methods that can rapidly separate, identify, and quantify isomeric glycans. We have developed a rapid-throughput method for chromatographic glycan profiling using microfluidic chip-based nanoflow liquid chromatography (nano-LC)/mass spectrometry. To demonstrate the utility of this method, we analyzed and compared serum samples from epithelial ovarian cancer cases (n = 46) and healthy control individuals (n = 48). Over 250 N-linked glycan compound peaks with over 100 distinct N-linked glycan compositions were identified. Statistical testing identified 26 potential glycan biomarkers based on both compositional and structure-specific analyses. Using these results, an optimized model was created incorporating the combined abundances of seven potential glycan biomarkers. The receiver operating characteristic (ROC) curve of this optimized model had an area under the curve (AUC) of 0.96, indicating robust discrimination between cancer cases and healthy controls. Rapid-throughput chromatographic glycan profiling was found to be an effective platform for structure-specific biomarker discovery.

Subject Keywords

Organic Chemistry, Analytical Chemistry, Biochemistry, General Medicine

URI

https://hdl.handle.net/11511/36572

Journal

JOURNAL OF CHROMATOGRAPHY A

DOI

https://doi.org/10.1016/j.chroma.2012.12.079

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

MTA-1 expression is associated with metastasis and epithelial to mesenchymal transition in colorectal cancer cells Cagatay, Seda Tuncay; Cimen, Ismail; SAVAŞ, BERNA; Banerjee, Sreeparna (Springer Science and Business Media LLC, 2013-04-01) Although metastasis associated protein 1 (MTA1) has been widely linked to tumor metastasis, the relevant mechanisms remain to be elucidated, especially in colorectal cancer (CRC). Here, we have investigated the link between MTA1, metastasis and epithelial-mesenchymal transition (EMT) in CRC. Eighteen normal colon tissues and 91 resected tumor samples were analyzed for MTA1 expression by immunohistochemistry (IHC). IHC indicated low or no nuclear MTA1 expression in the normal tissues and significantly higher...
Enzymic activity of the K5-type yeast killer toxin and its characterization Izgu, F; Altinbay, D; Sertkaya, A (Informa UK Limited, 2005-11-01) K5-type yeast killer toxin secreted by P. anomala NCYC 434 cells has a broad killing spectrum. Competitive inhibiton of killer activity showed that glucans, mainly the beta-1,3 glucan, represent the primary toxin binding site within the cell wall of sensitive cells. Its hydrolytic activity on laminarin in an exo-like fashion revealed that the toxin exerts its killing effect by exo-beta-1,3-glucanase activity. Its specific activity on laminarin was 120U/mg, and the Michaelis constants K-m, and V-max for lami...
Epigenetic Mechanisms Underlying the Dynamic Expression of Cancer-Testis Genes, PAGE2, -2B and SPANX-B, during Mesenchymal-to-Epithelial Transition Yilmaz-Ozcan, Sinem; Sade, Asli; Kucukkaraduman, Baris; Kaygusuz, Yasemin; Senses, Kerem Mert; Banerjee, Sreeparna; GÜRE, ALİ OSMAY (Public Library of Science (PLoS), 2014-09-17) Cancer-testis (CT) genes are expressed in various cancers but not in normal tissues other than in cells of the germline. Although DNA demethylation of promoter-proximal CpGs of CT genes is linked to their expression in cancer, the mechanisms leading to demethylation are unknown. To elucidate such mechanisms we chose to study the Caco-2 colorectal cancer cell line during the course of its spontaneous differentiation in vitro, as we found CT genes, in particular PAGE2, -2B and SPANX-B, to be up-regulated duri...
Label-free detection of telomerase activity using guanine electrochemical oxidation signal Eskiocak, Ugur; Ozkan-Ariksoysal, Dilsat; Ozsoz, Mehmet; Öktem, Hüseyin Avni (American Chemical Society (ACS), 2007-11-15) Telomerase is an important biomarker for cancer cells and its activation in 85% of all cancer types confers a clinical diagnostic value. A label-free electrochemical assay based on guanine oxidation signal to measure telomerase activity is described. This developed technology combined with a disposable sensor, carbon graphite electrode (CGE), and differential pulse voltammetry (DPV) was performed by using PCR amplicons with/without telomeric repeats as the guanine oxidation signal observed at +1.0 V measure...
Carbon supported and surfactant stabilized metal nanoparticle catalysts for direct methanol fuel cells Çelik, Çağlar; Gökağaç Arslan, Gülsün; Department of Chemistry (2005) Carbon supported surfactant, such as 1-decanethiol and octadecanethiol, stabilized platinum and platinum/ruthenium species have been prepared recently. In this thesis, for the first time, 1-hexanethiol has been used as an organic stabilizer for the preparation of carbon supported platinum and platinum/ruthenium nanoparticle catalysts. These new catalysts were employed for methanol oxidation reaction, which were used for direct methanol fuel cells. Cyclic voltammetry, X-ray photoelectron spectroscopy and tra...

Citation Formats

S. Hua et al., “Isomer-specific chromatographic profiling yields highly sensitive and specific potential N-glycan biomarkers for epithelial ovarian cancer,” JOURNAL OF CHROMATOGRAPHY A, pp. 58–67, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/36572.