Apolipoprotein A5 polymorphisms in Turkish population: association with serum lipid profile and risk of ischemic stroke

Date

2012-12-01

Author

CAN DEMİRDÖĞEN, BİRSEN
Sahin, Esra
Ozcelik, Aysun Turkanoglu
Bek, Semai
Demirkaya, Seref
Adalı, Orhan

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

246
views

0
downloads

Atherosclerosis, a major cause of ischemic stroke, may be associated with variability of triglyceride (TG) levels. Apolipoprotein A5 (APOA5) genetic polymorphisms are associated with altered TG levels. The objective of this study was to investigate the coding region polymorphisms S19W (rs3135506) and G185C (rs2075291) and the promoter region polymorphism -1131T > C (rs662799) of the APOA5 gene as risk factors for ischemic stroke in Turkish population. Study group consisted of 272 ischemic stroke patients and 123 controls. Genotypes were determined by real-time polymerase chain reaction (PCR) for S19W and PCR-restriction fragment length polymorphism analysis (PCR-RFLP) for -1131T > C and G185C. 19W allele frequency was 0.090 in stroke patients and 0.062 in controls (P = 0.191). Minor allele frequencies of -1131T > C and G185C in patients were 0.106 and 0.004, respectively, and were nearly the same in controls. Total cholesterol and LDL-cholesterol levels were significantly higher for stroke patients having at least one 19W allele compared to non-carriers. A significant difference was also found for LDL-cholesterol levels of stroke patients; higher in -1131C allele carriers compared to wild type patients. There was a trend for higher frequency of ischemic stroke among -1131C allele carrier hypertensive, diabetic or obese subjects compared to non-carriers. However, APOA5 genotypes were not associated with the risk of ischemic stroke by logistic regression analysis. The present study demonstrated that carrying rare alleles of APOA5 S19W, -1131T > C and G185C alone do not constitute a risk for ischemic stroke in the studied Turkish subjects.

Subject Keywords

Genetics, Molecular Biology, General Medicine

URI

https://hdl.handle.net/11511/40031

Journal

MOLECULAR BIOLOGY REPORTS

DOI

https://doi.org/10.1007/s11033-012-1926-z

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Gene expressions of Mn-SOD and GPx-1 in streptozotocin-induced diabetes: effect of antioxidants Sadi, Goekhan; Güray, Nülüfer Tülün (Springer Science and Business Media LLC, 2009-07-01) Increased oxidative stress and impaired antioxidant defense mechanisms are believed to be the important factors contributing to the pathogenesis and progression of diabetes mellitus. In this study, we have reported the effects of the streptozotocin-induced diabetes on the gene expression and the activities of two antioxidant enzymes, manganese superoxide dismutase (MnSOD) and glutathione peroxidase (GPx). We also studied the effects of two antioxidants, vitamin C and DL-alpha-lipoic acid (LA), on the system...
MTA-1 expression is associated with metastasis and epithelial to mesenchymal transition in colorectal cancer cells Cagatay, Seda Tuncay; Cimen, Ismail; SAVAŞ, BERNA; Banerjee, Sreeparna (Springer Science and Business Media LLC, 2013-04-01) Although metastasis associated protein 1 (MTA1) has been widely linked to tumor metastasis, the relevant mechanisms remain to be elucidated, especially in colorectal cancer (CRC). Here, we have investigated the link between MTA1, metastasis and epithelial-mesenchymal transition (EMT) in CRC. Eighteen normal colon tissues and 91 resected tumor samples were analyzed for MTA1 expression by immunohistochemistry (IHC). IHC indicated low or no nuclear MTA1 expression in the normal tissues and significantly higher...
Effect of vitamin C and lipoic acid on streptozotocin-induced diabetes gene expression: mRNA and protein expressions of Cu-ZnSOD and catalase Sadi, Goekhan; Yilmaz, Oekkes; Güray, Nülüfer Tülün (Springer Science and Business Media LLC, 2008-02-01) The involvement of oxidative stress in the pathogenesis of diabetes mellitus has been confirmed by numerous studies. In this study, the expression of two antioxidant enzymes, superoxide dismutase (SOD), and catalase which are involved in the detoxification of reactive oxygen species was studied in the streptozotocin-induced diabetic rat liver tissues. The enzyme assays showed a significant decrease in both enzymes activities compared to control animals. The RT-PCR and Western-blot analysis results demonstra...
Paraoxonase/arylesterase ratio, PON1 192Q/R polymorphism and PON1 status are associated with increased risk of ischernic stroke Demirdogen, Birsen Can; Turkanoglu, Aysun; Bek, Semai; Sanisoglu, Yavuz; Demirkaya, Seref; Vural, Okay; Arinc, Emel; Adalı, Orhan (Elsevier BV, 2008-01-01) Objectives: In recent years, importance of enzyme activity measurements, in addition to genotyping, in epidemiological studies relating paraoxonase 1 (PON1) and vascular disease was emphasized. This is the first report evaluating paraoxonase and arylesterase activities as risk factors for ischemic stroke. In addition, PON1 192Gln(Q)/Arg(R) and 55Leu(L)/Met(M) polymorphisms were also analyzed.
Regulation of biofilm formation bymarTinSalmonellaTyphimurium Eran, Zeynep; AKÇELİK, MUSTAFA; Yazici, Betul Cansu; Özcengiz, Gülay; AKÇELİK, NEFİSE (Springer Science and Business Media LLC, 2020-07-01) In this study, we aimed at identifying the regulatory role ofmarTgene, known as the regulator ofmisL, on 15 different biofilm-related genes inS.Typhimurium 14028 strain. We also tested the strains for their ability to form biofilm and determined the adherence characteristics of the wild type and the mutant strains of the organism on Caco-2 and HEp-2 cells. For comparative analyses of the candidate genes, individual gene mutations were created via antibiotic gene cassette insertion into each gene of interest...

Citation Formats

B. CAN DEMİRDÖĞEN, E. Sahin, A. T. Ozcelik, S. Bek, S. Demirkaya, and O. Adalı, “Apolipoprotein A5 polymorphisms in Turkish population: association with serum lipid profile and risk of ischemic stroke,” MOLECULAR BIOLOGY REPORTS, pp. 10459–10468, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40031.