Activation of type I interferon-dependent genes characterizes the "core response" induced by CpG DNA.

Date

2012-10-01

Author

Steinhagen, F
Meyer, C
Tross, D
Gürsel, Mayda
Maeda, T
Klaschik, S
Klinman, DM

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Synthetic ODNs expressing CpG motifs trigger an innate immune response via TLR9. pDCs are major effectors of this response. Two structurally distinct classes of CpG ODNs have been identified that differentially activate pDCs. "K" ODNs trigger the production of TNF-alpha and IL-6, whereas "D" ODNs preferentially induce the secretion of IFN-alpha. As K and D ODNs have distinct therapeutic effects, knowledge of their shared and sequence-specific activity is of considerable importance. This work uses the CAL-1 human pDC line to analyze the effect of CpG stimulation on gene expression. Genes up-regulated by both K and D ODNs (n = 92) were largely dependent on type I IFN signaling and characterized functionally by antiviral activity. K ODNs induced a short-term increase in IFN-alpha/beta production and uniquely up-regulated genes that supported antibacterial responses. In contrast, D ODNs triggered a persistent increase in IFN-alpha/beta production and uniquely up-regulated genes associated with metabolic functions. Thus, the core functionality of human pDCs mediated by TLR9 ligation rests on a type I IFN response that differs from the response induced by the structural elements unique to specific classes of ODNs. J. Leukoc. Biol. 92: 775-785; 2012.

Subject Keywords

Oligonucleotide, Gene regulation, TLR9, CAL-1, Human, pDC

URI

https://hdl.handle.net/11511/35945

Journal

Journal of leukocyte biology

DOI

https://doi.org/10.1189/jlb.1011522

Collections

Department of Biology, Article

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Citation Formats

F. Steinhagen et al., “Activation of type I interferon-dependent genes characterizes the “core response” induced by CpG DNA.,” Journal of leukocyte biology, pp. 775–85, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/35945.