A novel promising biomolecule immobilization matrix: Synthesis of functional benzimidazole containing conducting polymer and its biosensor applications

Date

2013-12-01

Author

Uzun, Sema Demirci
Unlu, Naime Akbasoglu
Sendur, Merve
Kanik, Fulya Ekiz
TİMUR, SUNA
Toppare, Levent Kamil

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

196
views

0
downloads

In order to construct a robust covalent binding between biomolecule and immobilization platform in biosensor preparation, a novel functional monomer 4-(4,7-di(thiophen-2-yl)-1H-benzo[d]imidazol-2yl)benzaldehyde (BIBA) was designed and successfully synthesized. After electropolymerization of this monomer, electrochemical and spectroelectrochemical properties were investigated in detail. To fabricate the desired biosensor, glucose oxidase (GOx) was immobilized as a model enzyme on the polymer coated graphite electrode with the help of glutaraldehyde (GA). During the immobilization step, an imine bond was formed between the free amino groups of enzyme and aldehyde group of polymer. The surface characterization and morphology were investigated to confirm bioconjugation by X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM) at each step of biosensor fabrication. The optimized biosensor shows good linearity between 0.02 mM and 1.20 mM and a low limit of detection (LOD) of 2.29 mu M. Kinetic parameters K-m(app) and I-max were determined as 0.94 mM and 10.91 mu A, respectively. The biosensor was tested for human blood serum samples.

Subject Keywords

Conducting Polymer, Biosensor, Enzyme Immobilization, Low Detection Limit, Glucose Level

URI

https://hdl.handle.net/11511/36399

Journal

COLLOIDS AND SURFACES B-BIOINTERFACES

DOI

https://doi.org/10.1016/j.colsurfb.2013.07.049

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

A flexible carbon nanofiber and conjugated polymer-based electrode for glucose sensing BULUT, Umut; Oyku Sayin, Vuslat; Altin, Yasin; Can Cevher, Sevki; Çırpan, Ali; Celik Bedeloglu, Ayse; Soylemez, Saniye (2023-01-01) Herein, a specific and stable biosensor for glucose using a flexible, modified electrode with a carbon nanofiber (CNF) and a novel conjugated polymer including three moieties of benzotriazole, benzodithiophene, and benzenediamine (P-BDT-BTz:BDA) as a platform was designed. For this purpose, polyacrylonitrile (PAN) nanofiber mats were obtained by a solution-based electrospinning method. PAN nanofiber mats were stabilized and carbonized to turn into carbon nanofibers and the sensing platform was formed by com...
A novel approach for the fabrication of a flexible glucose biosensor: The combination of vertically aligned CNTs and a conjugated polymer GOKOGLAN, Tugba Ceren; SOYLEMEZ, Saniye; KESİK, Melis; DOGRU, Itir Bakis; TUREL, Onur; YÜKSEL, Recep; Ünalan, Hüsnü Emrah; Toppare, Levent Kamil (2017-04-01) A novel flexible glucose biosensor using vertically aligned carbon nanotubes (VACNT) and a conjugated polymer (CP) was fabricated. A scaffold based on VACNT grown on aluminum foil (VACNT-Al foil) with poly (9,9-di-(2-ethylhexyl)-fluorenyl-2,7-diyl)-end capped with 2,5-diphenyl-1,2,4-oxadiazole (PFLO) was used as the immobilization matrix for the glucose biosensor. Glucose oxidase (GOx) was immobilized on a modified indium tin oxide (ITO) coated polyethylene terephthalate (PET) electrode surface. The biosens...
A benzimidazole-based conducting polymer and a PMMA-clay nanocomposite containing biosensor platform for glucose sensing EMRE, FATMA BİLGE; Kesik, Melis; Kanik, Fulya Ekiz; Akpinar, Hava Zekiye; Aslan-Gurel, Evren; Rossi, Rene M.; Toppare, Levent Kamil (2015-09-01) Development of materials composed of polymer-clay nanocomposites (PCN) and conducting polymers attracts great interest and preferred in various applications. Hereby, polymethylmethacrylate (PMMA) layered silicate nanocomposites were prepared by in-situ suspension polymerization by grafting PMMA with laponite using a suitable grafting agent. The properties of the as-synthesized PCN materials are characterized by differential scanning calorimetry (DSC), thermal gravimetry analysis (TGA) and gel permeation chr...
A new p and n dopable selenophene derivative and its electrochromic properties Ardahan, Gülben; Toppare, Levent Kamil; Department of Chemistry (2008) A novel electrically conducting polymer, poly(2-dodecyl-4,7-di(selenophen-2-yl)benzotriazole) (Poly(SBT)), containing selenophene as a strong donor and benzotriazole as a strong acceptor group was synthesized by electrochemical polymerization. Homopolymerization and copolymerization ( in the presence of 3,4-ethylenedioxythiophene (EDOT) ) was achieved in acetonitrile/ dichloromethane(95/5 v/v) with 0.1M tetrabutylammonium hexafluorophosphate (TBAPF6). The electrochemical and optical properties of homopolyme...
A novel conducting polymer based on terthienyl system bearing strong electron-withdrawing substituents and its electrochromic device application Asil Alptekin, Demet; Algı, Fatih; Önal, Ahmet Muhtar (Elsevier BV, 2008-7) A novel conducting polymer bearing strong electron-withdrawing substituents (EWS) directly attached to the 3,4-positions of the thiophene ring was synthesized by electrochemical polymerization of diethyl 2,5-di(thiophen-2-yl)thiophene-3,4-dicarboxylate (SSS-Diester). The polymer (PSSS-Diester) was characterized by cyclic voltammetry, FT-IR and UV-vis spectroscopy. The polymer has a reversible redox process and demonstrates a stable electrochromic behavior: reddish orange in the neutral state, brown in the i...

Citation Formats

S. D. Uzun, N. A. Unlu, M. Sendur, F. E. Kanik, S. TİMUR, and L. K. Toppare, “A novel promising biomolecule immobilization matrix: Synthesis of functional benzimidazole containing conducting polymer and its biosensor applications,” COLLOIDS AND SURFACES B-BIOINTERFACES, pp. 74–80, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/36399.