A novel approach for the fabrication of a flexible glucose biosensor: The combination of vertically aligned CNTs and a conjugated polymer

Date

2017-04-01

Author

GOKOGLAN, Tugba Ceren
SOYLEMEZ, Saniye
KESİK, Melis
DOGRU, Itir Bakis
TUREL, Onur
YÜKSEL, Recep
Ünalan, Hüsnü Emrah
Toppare, Levent Kamil

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A novel flexible glucose biosensor using vertically aligned carbon nanotubes (VACNT) and a conjugated polymer (CP) was fabricated. A scaffold based on VACNT grown on aluminum foil (VACNT-Al foil) with poly (9,9-di-(2-ethylhexyl)-fluorenyl-2,7-diyl)-end capped with 2,5-diphenyl-1,2,4-oxadiazole (PFLO) was used as the immobilization matrix for the glucose biosensor. Glucose oxidase (GOx) was immobilized on a modified indium tin oxide (ITO) coated polyethylene terephthalate (PET) electrode surface. The biosensor response at a potential of -0.7 V versus Ag wire was followed by the decrease in oxygen level as a result of enzymatic reaction. The biosensor exhibited a linear range between 0.02 mM and 0.5 mM glucose and kinetic parameters (K-M(app), I-max, limit of detection (LOD) and sensitivity) were estimated as 0.193 mM, 8.170 mu A, 7.035 x 10(-3) mM and 65.816 mu A/mM cm(2), respectively. Scanning electron microscopy (SEM) was used for surface characterization. The constructed biosensor was applied to determine the glucose content in several beverages.

Subject Keywords

Conjugated Polymer, Glucose Oxidase, Enzyme Electrode, Amperometric Biosensor, Vertically Aligned Carbon Nanotubes, Glucose Biosensor

URI

https://hdl.handle.net/11511/42240

Journal

FOOD CHEMISTRY

DOI

https://doi.org/10.1016/j.foodchem.2016.10.023

Collections

Department of Metallurgical and Materials Engineering, Article

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Citation Formats

T. C. GOKOGLAN et al., “A novel approach for the fabrication of a flexible glucose biosensor: The combination of vertically aligned CNTs and a conjugated polymer,” FOOD CHEMISTRY, pp. 299–305, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/42240.