Characterization and potential applications of immobilized glucose oxidase and polyphenol oxidase

Date

2007-07-01

Author

Ekinci, Olcun
Boyukbayram, A. Elif
Kiralp, Senem
Toppare, Levent Kamil
Yagci, Yusuf

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

151
views

0
downloads

Pyrrole functionalized polystyrene (PStPy) was copolymerized with pyrrole to obtain a conducting copolymer, P(PStPy-co-Py) which is used as the immobilization matrix. Glucose oxidase and polyphenol oxidase enzymes were immobilized via the entrapment method by electrochemical polymerization. Enzyme electrodes were prepared by electrolysis at a constant potential using sodium dodecyl sulfate (SDS) as the supporting electrolyte during the copolymerization of PStPy with pyrrole. Maximum reaction rates (V-max) and enzyme affinities (Michaelis-Menten constants, K-m) were determined for the enzyme entrapped both in polypyrrole (PPy) and P(PStPy-co-Py) matrices. Optimizations of enzyme electrodes were done by examining the effects of temperature and pH on enzymes' activities along with the shelf life and operational stability investigations. Glucose oxidase enzyme electrodes were used for human serum analysis and glucose determination in two brands of orange juices. Polyphenol oxidase enzyme electrodes were used for the determination of phenolics in red wines of Turkey.

Subject Keywords

Materials Chemistry, General Chemistry, Polymers and Plastics, Ceramics and Composites

URI

https://hdl.handle.net/11511/36750

Journal

JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY

DOI

https://doi.org/10.1080/10601320701407300

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Synthesis, characterization and electrochromic properties of copolymer of terephthalic acid bis-(thiophen-3-yl-methyl) thioester with thiophene Turkarslan, O; Erden, A; Sahin, E; Toppare, Levent Kamil (Informa UK Limited, 2006-01-01) Terephthalic acid bis-(thiophen-3-yl-methyl thioester) (TTMT) was synthesized via the reaction of thiophen-3-yl methanethiol with terephthaloyl dichloride. Nuclear magnetic resonance ( H-1-NMR) spectroscopy and Fourier transform infrared (FTIR) spectroscopy were utilized for the characterization of the monomer. Electrochemical copolymerization of TTMT with thiophene in acetonitrile/boron trifluoride diethyl etherate (AN/BFEE) (8:2, v/v) solvent mixture was realized by using tetrabutylammonium tetrafluorobor...
Comparison of Natural Halloysite With Synthetic Carbon Nanotubes in Poly(lactic acid) Based Composites Erpek, Canan Esma Yeniova; ÖZKOÇ, GÜRALP; Yılmazer, Ülkü (Wiley, 2017-11-01) The objective of this study is to compare the mechanical properties, structure and degradability of the nanocomposites prepared with tubular nanofillers, halloysite (HNT) and carbon nanotube (CNT) in poly(lactic acid) (PLA), and thermoplastic polyurethane (TPU) toughened PLA (T-PLA) matrices. In the PLA matrix, CNT increased, whereas HNT decreased the tensile strength with increasing filler content. Also, the elongation at break and impact strength decreased with increasing CNT content, but these properties...
Preparation of a conducting flexible material from silane coupling agent and hydroxyl terminated polybutadiene rubber by hydrolysis and condensation Karatas, Y; Toppare, Levent Kamil; Tincer, T (Informa UK Limited, 2003-01-01) Synthesis and characterization of a flexible polymer produced from silane coupling agent (SCA) and hydroxyl terminated polybutadiene (HTPB) were performed. Mechanical properties of chemically and electrochemically prepared conducting composites synthesized from this polymer were investigated. Conductivities of the composites were also measured. Polypyrrole enhanced the mechanical properties of the chemically prepared conducting composite. Doping with iodine greatly changed the conductivity of the composite....
Synthesis and optical properties of light-emitting π-conjugated polymers containing biphenyl and dithienosilole LIAO, Liang; Çırpan, Ali; CHU, Qinghui; KARASZ, Frank E.; PANG, YI (Wiley, 2007-05-15) Copolymers containing oligo(phenylene vinylene) (2.5), fluorene, and 4,4-dihexyldithienosilole (DTS) units were synthesized and characterized. The pi-conjugated monomers were joined with the palladium(0)-catalyzed Suzuki-Miyaura coupling reaction, thus forming either biphenyl- or phenyl-thiophene linkages. These polymers were photoluminescent, with the fluorescent quantum efficiency between 54 and 63% and with),lambda(max) for fluorescence at similar to 448 nm in tetrahydrofuran. The presence of 5% DTS in t...
A New Amperometric Alcohol Oxidase Biosensor Based on Conducting Polymer of (4,7-Dithien-2-yl-2, 1,3-benzothiadiazole) Tanriverdi, Simla; Tuncagil, Sevinc; Toppare, Levent Kamil (Informa UK Limited, 2012-01-01) Amperometric biosensors based on alcohol oxidase (AOX) immobilized on the conducting polymers of 3,4-ethylenedioxythiophene (PEDOT) and 4,7-dithien-2-yl-2,1,3-benzothiadiazole (TBTD) were constructed. Proposed biosensors were characterized using ethanol as the substrate and the responses were measured. Kinetic parameters, I-max, K-m and sensitivity were determined for the biosensors with or without TBTD. Optimization studies for TBTD thickness, PEDOT amount and pH were carried out. The biosensing capacity w...

Citation Formats

O. Ekinci, A. E. Boyukbayram, S. Kiralp, L. K. Toppare, and Y. Yagci, “Characterization and potential applications of immobilized glucose oxidase and polyphenol oxidase,” JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY, pp. 801–808, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/36750.