Characterization and potential applications of immobilized glucose oxidase and polyphenol oxidase

Ekinci, Olcun
Boyukbayram, A. Elif
Kiralp, Senem
Toppare, Levent Kamil
Yagci, Yusuf
Pyrrole functionalized polystyrene (PStPy) was copolymerized with pyrrole to obtain a conducting copolymer, P(PStPy-co-Py) which is used as the immobilization matrix. Glucose oxidase and polyphenol oxidase enzymes were immobilized via the entrapment method by electrochemical polymerization. Enzyme electrodes were prepared by electrolysis at a constant potential using sodium dodecyl sulfate (SDS) as the supporting electrolyte during the copolymerization of PStPy with pyrrole. Maximum reaction rates (V-max) and enzyme affinities (Michaelis-Menten constants, K-m) were determined for the enzyme entrapped both in polypyrrole (PPy) and P(PStPy-co-Py) matrices. Optimizations of enzyme electrodes were done by examining the effects of temperature and pH on enzymes' activities along with the shelf life and operational stability investigations. Glucose oxidase enzyme electrodes were used for human serum analysis and glucose determination in two brands of orange juices. Polyphenol oxidase enzyme electrodes were used for the determination of phenolics in red wines of Turkey.


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Vargun, Elif; SANKIR, MEHMET; Aran, Bengi; DEMİRCİ SANKIR, NURDAN; Usanmaz, Ali (Informa UK Limited, 2010-01-01)
A series of poly(methyl methacrylate-co-hydroxyethyl methacrylate) (PMMA-co-PHEMA), copolymers were synthesized by an emulsion polymerization technique. Copolymer compositions were determined by FT-IR and 1H-NMR spectroscopy. It was found that comonomer ratios used in the recipes were comparable within the actual copolymers. Glass transition temperatures (Tg) of PMMA-co-PHEMA copolymers were varied from 119 degrees C to 100 degrees C by increasing HEMA content. Thermogravimetric analysis showed that the cop...
Synthesis and properties of thermotropic liquid crystalline copolyesters containing p-hydroxyphenylacetic acid and m-hydroxybenzoic acid units
Yerlikaya, Z; Aksoy, S; Bayramlı, Erdal (Informa UK Limited, 2006-03-01)
A series of copolyesters based on p-acetoxybenzoic acid (p-ABA), m-acetoxybenzoic acid (m-ABA), and p-acetoxyphenylacetic acid (p-APAA) were prepared by a modified melt polycondensation reaction. The effect of linear p-ABA structure, kink m-ABA and flexible p-APAA moiety on the properties of copolyesters were systematically investigated. The copolyesters were characterized by DSC, thermogravimetric analysis, 1 H-NMR, polarized optical microscopy, X-ray diffraction, and intrinsic viscosity measurements. The ...
Synthesis and Characterization of Conducting Copolymers of Bisphenol A-Diglycidyl Ether with Thiophene Side-Groups and Pyrrole
Sahmetlioglu, Ertugrul; Varol, Ramazan; Toppare, Levent Kamil; Yuruk, Huseyin (Informa UK Limited, 2009-01-01)
Copolymers of bisphenol A-diglycidyl ether with thiophene side-groups and pyrrole were synthesized by electrochemical polymerization. Bisphenol A-diglycidyl ether with thiophene side-groups (DGEBATh) was obtained from the reaction between bisphenol A-diglycidyl ether (DGEBA) and thiophene-3-acetic acid. The syntheses of copolymers of DGEBATh and pyrrole were achieved electrochemically using three different supporting electrolytes, p-toluene sulfonic acid (PTSA), sodium dodecyl sulfate (SDS) and tetrabutylam...
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Turkarslan, O; Erden, A; Sahin, E; Toppare, Levent Kamil (Informa UK Limited, 2006-01-01)
Terephthalic acid bis-(thiophen-3-yl-methyl thioester) (TTMT) was synthesized via the reaction of thiophen-3-yl methanethiol with terephthaloyl dichloride. Nuclear magnetic resonance ( H-1-NMR) spectroscopy and Fourier transform infrared (FTIR) spectroscopy were utilized for the characterization of the monomer. Electrochemical copolymerization of TTMT with thiophene in acetonitrile/boron trifluoride diethyl etherate (AN/BFEE) (8:2, v/v) solvent mixture was realized by using tetrabutylammonium tetrafluorobor...
Characteristics of Impact Modified Polystyrene/Organoclay Nanocomposites
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The poor impact resistance of Polystyrene (PS) was enhanced by the addition of elastomeric material, SEBS-g-MA. To prevent the reduction in strength and stiffness, organoclay Cloisite (R) 25A was used as filler and introduced into the matrix by a corotating twin screw extruder. Throughout the study, the clay content was kept at 2 wt%, whereas the content of SEBS-g-MA was varied between 5 and 40 wt%. It was found that Cloisite (R) 25A displays well dispersion in the ternary nanocomposites and the degree of d...
Citation Formats
O. Ekinci, A. E. Boyukbayram, S. Kiralp, L. K. Toppare, and Y. Yagci, “Characterization and potential applications of immobilized glucose oxidase and polyphenol oxidase,” JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY, pp. 801–808, 2007, Accessed: 00, 2020. [Online]. Available: