Identification of population dynamics in sulfate-reducing consortia on exposure to sulfate

Date

2006-12-01

Author

İçgen, Bülent

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

200
views

0
downloads

The microbial population structure and function of a mixed culture of sulfate-reducing bacteria (SRB) maintained in anaerobic continuous bioreactors were tracked before and after a major perturbation, which involved the addition of sulfate to the influent of a bioreactor when operated at steady state at 35 degrees C, pH 7.8 and a 2.5 day residence time with feed stream containing 10 and 15 kg m(-3) sulfate as terminal electron acceptor and 19.6 and 29.4 kg m(-3) 4 ethanol as carbon source and electron donor, respectively. The population structure determined by fluorescence in situ hybridization (FISH), by using 16S rRNA-targeted oligonucleotide probes, was linked to the functional performance of the SRB in the reactor. Hybridization analysis using these 16S rRNA-targeted oligonucleotide probes revealed that a high concentration of sulfate was toxic for Desulfobacterium and Desulfobulbus. On the other hand, the Desulfococcus group was found to be the most dominant group of SRB in the feed stream containing 15 kg m(-3) sulfate as terminal electron acceptor and 29.4 kg m(-3) ethanol as carbon source and electron donor. (c) 2006 Published by Elsevier Masson SAS.

Subject Keywords

Molecular Biology, Microbiology, General Medicine

URI

https://hdl.handle.net/11511/37022

Journal

Research in Microbiology

DOI

https://doi.org/10.1016/j.resmic.2006.08.003

Collections

Department of Environmental Engineering, Article

Suggestions

OpenMETU
Core

Exposure to sulfide causes populations shifts in sulfate-reducing consortia İçgen, Bülent (Elsevier BV, 2006-10-01) The shift in the community structure of a mixed culture of sulfate-reducing bacteria (SRB) at 0.5, 0.75, 1, and 1.5 kg m(-3) sulfide loadings was investigated in an anaerobic continuous bioreactor used for treatment of sulfate-containing wastewater by fluorescence in situ hybridization (FISH), using SRB species-specific and group-specific 16S rRNA-targeting probes. Hybridization analysis using these 16S rRNA-targeted oligonucleotide probes revealed that sulfide was toxic for Desulfonema, Desulfobulbus spp. ...
Regulation of crystal protein biosynthesis by Bacillus thuringiensis: II. Effects of carbon and nitrogen sources. Içgen, Y; İçgen, Bülent; Özcengiz, Gülay (Elsevier BV, 2002-11-01) The regulation of crystal protein production in Bacillus thuringiensis 81 by sources of carbon and nitrogen was investigated. The highest titers of toxin were obtained on sucrose, lactose and inulin which also supported sporulation. Whey and molasses were also potential carbon substrates for toxin production. Other carbohydrates including glucose, glycerol, maltose, starch and dextrin yielded lower amounts of toxin. Nitrogen sources were found to exert the most profound controls. Peptone was the best organi...
Evaluation of symbiotic effectiveness of various Rhizobium cicer strains İçgen, Bülent; Özcengiz, Gülay (Elsevier BV, 2002-07-01) Five local and seven standard strains of Rhizobium cicer were compared in terms of their efficiency in increasing the nitrogen content of the chickpea. Shoot dry weight, nodule number, nodule dry weight, protein and total nitrogen contents were taken as the parameters of plant productivity. Different combinations of the strains that were found to be promising (385, 620, Y-29 and 3379) were next employed. The maximum increase in total nitrogen content was only 3.5-fold in single infection whereas an increase...
Regulation of crystal protein biosynthesis by Bacillus thuringiensis: I. Effects of mineral elements and pH ICGEN, Y; Icgen, BÜLENT; Özcengiz, Gülay (Elsevier BV, 2002-11-01) Crystal protein synthesis by a local isolate of Bacillus thuringiensis was monitored and compared in association with growth and sporulation in media differing in mineral element content. Mg and Cu were the most important metals for the biosynthesis of 135 kDa and 65 kDa toxin components in that the former was essential and the latter was greatly stimulatory at 10(-6) to 10(-7) M concentration. Also the inclusion of Mn favored toxin production at concentrations ranging from 3 x 10(-4) to 10(-5) M. The omiss...
Evaluation of high hydrostatic pressure sensitivity of Staphylococcus aureus and Escherichia coli O157 : H7 by differential scanning calorimetry Alpas, Hami; BOZOGLU, F; KALETUNC, G (Elsevier BV, 2003-11-01) Differential scanning calorimetry (DSC) was used to evaluate the relative high hydrostatic pressure (HHP) resistances of bacterial strains from Staphylococcus aureus and Escherichia coli O157:H7 in vivo. The total apparent enthalpy change and thermal stability were two DSC parameters used to compare bacterial strains of untreated control and pressure-treated bacteria. DSC thermograms indicated that ribosomal denaturation appears to be a major factor in cell death by both thermal and high pressure treatments...

Citation Formats

B. İçgen, “Identification of population dynamics in sulfate-reducing consortia on exposure to sulfate,” Research in Microbiology, pp. 922–927, 2006, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37022.