Exposure to sulfide causes populations shifts in sulfate-reducing consortia

The shift in the community structure of a mixed culture of sulfate-reducing bacteria (SRB) at 0.5, 0.75, 1, and 1.5 kg m(-3) sulfide loadings was investigated in an anaerobic continuous bioreactor used for treatment of sulfate-containing wastewater by fluorescence in situ hybridization (FISH), using SRB species-specific and group-specific 16S rRNA-targeting probes. Hybridization analysis using these 16S rRNA-targeted oligonucleotide probes revealed that sulfide was toxic for Desulfonema, Desulfobulbus spp. and the Desulfobacteriaceae group, although it was not toxic for Desulfobacter, Desulfotomaculum, Desulfobacterium spp. or the Desulfovibrionaceae group. On the other hand, only a high concentration of sulfide of 1.5 kg m(-3) was found to be toxic for the Desulfococcus group in the bioreactor. When the sulfide in the feed was 1.00 kg m(-3) the sulfate-reducing capacity of the system decreased, and this decrease was more pronounced when the inlet sulfide was further increased to 1.5 kg m(-3).
Research in Microbiology


Identification of population dynamics in sulfate-reducing consortia on exposure to sulfate
İçgen, Bülent (Elsevier BV, 2006-12-01)
The microbial population structure and function of a mixed culture of sulfate-reducing bacteria (SRB) maintained in anaerobic continuous bioreactors were tracked before and after a major perturbation, which involved the addition of sulfate to the influent of a bioreactor when operated at steady state at 35 degrees C, pH 7.8 and a 2.5 day residence time with feed stream containing 10 and 15 kg m(-3) sulfate as terminal electron acceptor and 19.6 and 29.4 kg m(-3) 4 ethanol as carbon source and electron donor...
Regulation of crystal protein biosynthesis by Bacillus thuringiensis: II. Effects of carbon and nitrogen sources.
Içgen, Y; İçgen, Bülent; Özcengiz, Gülay (Elsevier BV, 2002-11-01)
The regulation of crystal protein production in Bacillus thuringiensis 81 by sources of carbon and nitrogen was investigated. The highest titers of toxin were obtained on sucrose, lactose and inulin which also supported sporulation. Whey and molasses were also potential carbon substrates for toxin production. Other carbohydrates including glucose, glycerol, maltose, starch and dextrin yielded lower amounts of toxin. Nitrogen sources were found to exert the most profound controls. Peptone was the best organi...
Regulation of crystal protein biosynthesis by Bacillus thuringiensis: I. Effects of mineral elements and pH
ICGEN, Y; Icgen, BÜLENT; Özcengiz, Gülay (Elsevier BV, 2002-11-01)
Crystal protein synthesis by a local isolate of Bacillus thuringiensis was monitored and compared in association with growth and sporulation in media differing in mineral element content. Mg and Cu were the most important metals for the biosynthesis of 135 kDa and 65 kDa toxin components in that the former was essential and the latter was greatly stimulatory at 10(-6) to 10(-7) M concentration. Also the inclusion of Mn favored toxin production at concentrations ranging from 3 x 10(-4) to 10(-5) M. The omiss...
Evaluation of symbiotic effectiveness of various Rhizobium cicer strains
İçgen, Bülent; Özcengiz, Gülay (Elsevier BV, 2002-07-01)
Five local and seven standard strains of Rhizobium cicer were compared in terms of their efficiency in increasing the nitrogen content of the chickpea. Shoot dry weight, nodule number, nodule dry weight, protein and total nitrogen contents were taken as the parameters of plant productivity. Different combinations of the strains that were found to be promising (385, 620, Y-29 and 3379) were next employed. The maximum increase in total nitrogen content was only 3.5-fold in single infection whereas an increase...
Nutritional and cultural parameters influencing antidipteran delta endotoxin production
ÖZKAN, MELEK; Dilek, Filiz Bengü; Yetiş, Ülkü; Özcengiz, Gülay (Elsevier BV, 2003-01-01)
In this study, various nutritional and cultural parameters influencing diptera-specific delta-endotoxin synthesis by Bacillus thuringiensis subsp. israelensis HD500 were investigated. Of various inorganic nitrogen sources, the highest yields of Cry11Aa and Cry4Ba proteins were obtained on (NH4)(2)HPO4. Among carbon sources, inulin, dextrin, maltose, lactose, sucrose, whey and glycerol were all stimulatory, while glucose, starch and molasses were suppressive. High concentrations of inorganic phosphate (50 to...
Citation Formats
B. İçgen, “Exposure to sulfide causes populations shifts in sulfate-reducing consortia,” Research in Microbiology, pp. 784–791, 2006, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/44035.