Regulation of crystal protein biosynthesis by Bacillus thuringiensis: II. Effects of carbon and nitrogen sources.

Date

2002-11-01

Author

Içgen, Y
İçgen, Bülent
Özcengiz, Gülay

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The regulation of crystal protein production in Bacillus thuringiensis 81 by sources of carbon and nitrogen was investigated. The highest titers of toxin were obtained on sucrose, lactose and inulin which also supported sporulation. Whey and molasses were also potential carbon substrates for toxin production. Other carbohydrates including glucose, glycerol, maltose, starch and dextrin yielded lower amounts of toxin. Nitrogen sources were found to exert the most profound controls. Peptone was the best organic nitrogen source, supporting optimum production and sporulation as well as high cell density. The formation of CryI and CryII toxin proteins was found to be differentially regulated by the inorganic nitrogenous compounds incorporated into the medium. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.

Subject Keywords

Molecular Biology, Microbiology, General Medicine

URI

https://hdl.handle.net/11511/36785

Journal

Research in microbiology

DOI

https://doi.org/10.1016/s0923-2508(02)01366-9

Collections

Department of Environmental Engineering, Article

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Citation Formats

Y. Içgen, B. İçgen, and G. Özcengiz, “Regulation of crystal protein biosynthesis by Bacillus thuringiensis: II. Effects of carbon and nitrogen sources.,” Research in microbiology, pp. 605–9, 2002, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/36785.