Phenol biosensor based on electrochemically controlled integration of tyrosinase in a redox polymer

Date

2007-06-01

Author

Yildiz, Huseyin Bekir
Castillo, Jaime
Guschin, Dmitrii A.
Toppare, Levent Kamil
Schuhmann, Wolfgang

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

128
views

0
downloads

An amperometric biosensor for the detection of phenolic compounds was developed based on the immobilization of tyrosinase within an Os-complex functionalized electrodeposition polymer. Integration of tyrosinase within the redox polymer assures efficient catechol recycling between the enzyme and the polymer bound redox sites. The non-manual immobilization procedure improves the reproducibility of fabrication process, greatly reduces the desorption of the enzyme from the immobilization layer, and, most importantly prevents fast inactivation of the enzyme by its substrate due to fast redox cycling.

Subject Keywords

Analytical Chemistry

URI

https://hdl.handle.net/11511/37149

Journal

MICROCHIMICA ACTA

DOI

https://doi.org/10.1007/s00604-007-0768-1

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Determination of Arsenobetaine in Fish Tissue by Species Specific Isotope Dilution LC-LTQ-Orbitrap-MS and Standard Addition LC-ICPMS Yang, Lu; Ding, Jianfu; Maxwell, Paulette; McCooeye, Margaret; Windust, Anthony; Ouerdane, Laurent; Bakirdere, Sezgin; Willie, Scott; Mester, Zoltan (American Chemical Society (ACS), 2011-05-01) An accurate and precise method for the determination of arsenobetaine (AsB, (CH(3))(3)(+)AsCH(2)COO(-)) in fish samples using exact matching species specific isotope dilution (ID) liquid chromatography LTQ:Orbitrap mass spectrometry (LC-LTQ-Orbitrap-MS) and standard addition LC inductively coupled plasma mass spectrometry (LC-ICPMS) is described. Samples were extracted by sonication for 30 min with high purity deionized water. An in-house synthesized (13)C enriched AsB spike was used for species specific ID...
Determination of silver by chemical vapour generation and atomic absoption spectrometry Öztürk, Çağla Pınar; Ataman, Osman Yavuz; Department of Chemistry (2004) A method for determination of silver has been developed based on chemical vapour generation atomic absorption spectrometry (CVGAAS). Volatile species of silver in acidified medium were generated by the reduction of sodium tetrahydroborate; these species were sent to a flame-heated quartz tube atomizer (QTA) following isolation by using a gas-liquid separator. Flow injection (FI) was used for sample introduction. Optimization of parameters such as; concentrations of acid and NaBH4 concentration, flow rates o...
PRECONCENTRATION OF SOME TRACE-METALS FROM SEA-WATER ON A MERCAPTO-MODIFIED SILICA-GEL Volkan, Mürvet; Ataman, Osman Yavuz (1987-10-01) Silica modified by the addition of mercapto chelating groups has been developed for the pre-concentration of trace metals from natural waters. This material removes cadmium, copper, lead and zinc from aqueous solution and can be employed for the pre-concentration of these metals by both column and batch techniques. Under column and batch conditions recoveries larger than 95% were common. For batch extractions of cadmium, zinc, copper and lead from sea water, recoveries of 91 ± 5, 98 ± 4, 97 ± 4 and 96 ± 5%,...
A Diverse-Stimuli Responsive Chemiluminescent Probe with Luminol Scaffold and Its Electropolymerization Asil Alptekin, Demet; Algi, Fatih; Önal, Ahmet Muhtar (Wiley, 2010-10-01) A diverse-stimuli responsive chemiluminescent system, SNS-Lum, and its electropolymerization to give PSNS-Lum, which is the first example of conjugated polymers with pendant luminol arms inducing chemiluminescence in the presence of super oxide radical anion under neutral conditions, are highlighted.
Use of multi-walled carbon nanotubes in matrix solid phase dispersion extraction combined with gas chromatography Njie, Njaw; Aygün, Rüveyde Sezer; Department of Chemistry (2008) The use of Multi-Walled Carbon Nanotubes (MWCNT) as solid sorbent in Matrix Solid-Phase Dispersion (MSPD) extraction and preconcentration method was presented to determine some commonly used organophosphorus insecticides/OPIs in honey samples using a Gas Chromatography Flame Ionization Detector (GC-FID). OPIs are poisonous compounds used to kill insects and rodents by affecting their nervous system. The limit of detections obtained after MSPD extraction were 7.0 ng/g for Malathion, Malaoxon and Fenitrothion...

Citation Formats

H. B. Yildiz, J. Castillo, D. A. Guschin, L. K. Toppare, and W. Schuhmann, “Phenol biosensor based on electrochemically controlled integration of tyrosinase in a redox polymer,” MICROCHIMICA ACTA, pp. 27–34, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37149.