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Phenol biosensor based on electrochemically controlled integration of tyrosinase in a redox polymer

Yildiz, Huseyin Bekir
Castillo, Jaime
Guschin, Dmitrii A.
Toppare, Levent Kamil
Schuhmann, Wolfgang
An amperometric biosensor for the detection of phenolic compounds was developed based on the immobilization of tyrosinase within an Os-complex functionalized electrodeposition polymer. Integration of tyrosinase within the redox polymer assures efficient catechol recycling between the enzyme and the polymer bound redox sites. The non-manual immobilization procedure improves the reproducibility of fabrication process, greatly reduces the desorption of the enzyme from the immobilization layer, and, most importantly prevents fast inactivation of the enzyme by its substrate due to fast redox cycling.