Phenol biosensor based on electrochemically controlled integration of tyrosinase in a redox polymer

Yildiz, Huseyin Bekir
Castillo, Jaime
Guschin, Dmitrii A.
Toppare, Levent Kamil
Schuhmann, Wolfgang
An amperometric biosensor for the detection of phenolic compounds was developed based on the immobilization of tyrosinase within an Os-complex functionalized electrodeposition polymer. Integration of tyrosinase within the redox polymer assures efficient catechol recycling between the enzyme and the polymer bound redox sites. The non-manual immobilization procedure improves the reproducibility of fabrication process, greatly reduces the desorption of the enzyme from the immobilization layer, and, most importantly prevents fast inactivation of the enzyme by its substrate due to fast redox cycling.


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An accurate and precise method for the determination of arsenobetaine (AsB, (CH(3))(3)(+)AsCH(2)COO(-)) in fish samples using exact matching species specific isotope dilution (ID) liquid chromatography LTQ:Orbitrap mass spectrometry (LC-LTQ-Orbitrap-MS) and standard addition LC inductively coupled plasma mass spectrometry (LC-ICPMS) is described. Samples were extracted by sonication for 30 min with high purity deionized water. An in-house synthesized (13)C enriched AsB spike was used for species specific ID...
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The use of Multi-Walled Carbon Nanotubes (MWCNT) as solid sorbent in Matrix Solid-Phase Dispersion (MSPD) extraction and preconcentration method was presented to determine some commonly used organophosphorus insecticides/OPIs in honey samples using a Gas Chromatography Flame Ionization Detector (GC-FID). OPIs are poisonous compounds used to kill insects and rodents by affecting their nervous system. The limit of detections obtained after MSPD extraction were 7.0 ng/g for Malathion, Malaoxon and Fenitrothion...
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A method for determination of silver has been developed based on chemical vapour generation atomic absorption spectrometry (CVGAAS). Volatile species of silver in acidified medium were generated by the reduction of sodium tetrahydroborate; these species were sent to a flame-heated quartz tube atomizer (QTA) following isolation by using a gas-liquid separator. Flow injection (FI) was used for sample introduction. Optimization of parameters such as; concentrations of acid and NaBH4 concentration, flow rates o...
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A Diverse-Stimuli Responsive Chemiluminescent Probe with Luminol Scaffold and Its Electropolymerization
Asil Alptekin, Demet; Algi, Fatih; Önal, Ahmet Muhtar (Wiley, 2010-10-01)
A diverse-stimuli responsive chemiluminescent system, SNS-Lum, and its electropolymerization to give PSNS-Lum, which is the first example of conjugated polymers with pendant luminol arms inducing chemiluminescence in the presence of super oxide radical anion under neutral conditions, are highlighted.
Citation Formats
H. B. Yildiz, J. Castillo, D. A. Guschin, L. K. Toppare, and W. Schuhmann, “Phenol biosensor based on electrochemically controlled integration of tyrosinase in a redox polymer,” MICROCHIMICA ACTA, pp. 27–34, 2007, Accessed: 00, 2020. [Online]. Available: